@ARTICLE{10726870,
  author={Detrez, Nicolas and Burhan, Sazgar and Rewerts, Katarina and Kren, Jessica and Buschschlüter, Steffen and Theisen-Kunde, Dirk and Bonsanto, Matteo Mario and Huber, Robert and Brinkmann, Ralf},
  journal={IEEE Transactions on Biomedical Engineering}, 
  title={Flow-controlled air-jet for in vivo quasi steady-state and dynamic elastography with MHz optical coherence tomography}, 
  year={2024},
  volume={},
  number={},
  pages={1-12},
  keywords={Force;Biomedical measurement;Pressure measurement;In vivo;Steady-state;Generators;Elastography;Valves;Force measurement;Optical coherence tomography;Air-Jet;Air-Puff;Optical Coherence Elastography;Stiffness;Tissue Mechanics;Young's Modulus},
  doi={10.1109/TBME.2024.3484676}}

@article{Draxinger:24,
author = {Wolfgang Draxinger and Nicolas Detrez and Paul Strenge and Veit Danicke and Dirk Theisen-Kunde and Lion Sch\"{u}tzeck and Sonja Spahr-Hess and Patrick Kuppler and Jessica Kren and Wolfgang Wieser and Matteo Mario Bonsanto and Ralf Brinkmann and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {Brain imaging; Imaging systems; In vivo imaging; Magnetic resonance imaging; Speckle imaging; Spectral domain optical coherence tomography},
number = {10},
pages = {5960--5979},
publisher = {Optica Publishing Group},
title = {Microscope integrated MHz optical coherence tomography system for neurosurgery: development and clinical in-vivo imaging},
volume = {15},
month = {Oct},
year = {2024},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-15-10-5960},
doi = {10.1364/BOE.530976},
abstract = {Neurosurgical interventions on the brain are impeded by the requirement to keep damages to healthy tissue at a minimum. A new contrast channel enhancing the visual separation of malign tissue should be created. A commercially available surgical microscope was modified with adaptation optics adapting the MHz speed optical coherence tomography (OCT) imaging system developed in our group. This required the design of a scanner optics and beam delivery system overcoming constraints posed by the mechanical and optical parameters of the microscope. High quality volumetric OCT C-scans with dense sample spacing can be acquired in-vivo as part of surgical procedures within seconds and are immediately available for post-processing.},
}

@article{RN5506,
   author = {Rahn, U.;Rahn, C. D.;Arora, S.;Ng, E.;Kozak, I.;Chhablani, J.;Miura, Y and Group, Subthreshold Laser Planning},
   title = {Variability of thermal subthreshold retinal laser treatment plans},
   journal = {Sci Rep},
   volume = {14},
   number = {1},
   pages = {22723},
   ISSN = {2045-2322},
   DOI = {10.1038/s41598-024-73763-4},
   year = {2024},
   type = {Journal Article}
}

@article{10.1167/tvst.13.9.28,
    author = {Kleyman, Viktoria and Eggert, Sophie and Schmidt, Christian and Schaller, Manuel and Worthmann, Karl and Brinkmann, Ralf and Müller, Matthias A.},
    title = "{Model Predictive Temperature Control for Retinal Laser Treatments}",
    journal = {Translational Vision Science & Technology},
    volume = {13},
    number = {9},
    pages = {28-28},
    year = {2024},
    month = {09},
    abstract = "{   Manual, individual adjustment of the laser power in retinal laser therapies is time-consuming, is inaccurate with respect to uniform effects, and can only prevent over- or undertreatment to a limited extent. Automatic closed-loop temperature control allows for similar temperatures at each irradiated spot despite varying absorption. This is of crucial importance for subdamaging hyperthermal treatments with no visible effects and the safety of photocoagulation with short irradiation times. The aim of this work is to perform extensive experiments on porcine eye explants to demonstrate the benefits of automatic control in retinal laser treatments.    To ensure a safe and reliable temperature rise, we utilize a model predictive controller. For model predictive control, the current state and the spot-dependent absorption coefficients are estimated by an extended Kalman filter (EKF). Therein, optoacoustic measurements are used to determine the temperature rise at the irradiated areas in real time. We use fluorescence vitality stains to measure the lesion size and validate the proposed control strategy.    By comparing the lesion size with temperature values for cell death, we found that the EKF accurately estimates the peak temperature. Furthermore, the proposed closed-loop control scheme works reliably with regard to similar lesion sizes despite varying absorption with a smaller spread in lesion size compared to open-loop control.    Our closed-loop control approach enables a safe subdamaging treatment and lowers the risk for over- and undertreatment for mild coagulations in retinal laser therapies.    We demonstrate that modern control strategies have the potential to improve retinal laser treatments for several diseases.  }",
    issn = {2164-2591},
    doi = {10.1167/tvst.13.9.28},
    url = {https://doi.org/10.1167/tvst.13.9.28},
    eprint = {https://arvojournals.org/arvo/content\_public/journal/tvst/938688/i2164-2591-13-9-28\_1727347017.43447.pdf},
}

@article{https://doi.org/10.1002/smll.202401472,
author = {Karpf, Sebastian and Glöckner Burmeister, Nina and Dubreil, Laurence and Ghosh, Shayantani and Hollandi, Reka and Pichon, Julien and Leroux, Isabelle and Henkel, Alessandra and Lutz, Valerie and Jurkevičius, Jonas and Latshaw, Alexandra and Kilin, Vasyl and Kutscher, Tonio and Wiggert, Moritz and Saavedra-Villanueva, Oscar and Vogel, Alfred and Huber, Robert A. and Horvath, Peter and Rouger, Karl and Bonacina, Luigi},
title = {Harmonic Imaging of Stem Cells in Whole Blood at GHz Pixel Rate},
journal = {Small},
volume = {n/a},
number = {n/a},
pages = {2401472},
keywords = {fiber lasers, harmonic imaging, multiphoton microscopy, nanoparticles, regenerative medicine, SHG, SLIDE},
doi = {https://doi.org/10.1002/smll.202401472},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.202401472},
eprint = {https://onlinelibrary.wiley.com/doi/pdf/10.1002/smll.202401472},
abstract = {Abstract The pre-clinical validation of cell therapies requires monitoring the biodistribution of transplanted cells in tissues of host organisms. Real-time detection of these cells in the circulatory system and identification of their aggregation state is a crucial piece of information, but necessitates deep penetration and fast imaging with high selectivity, subcellular resolution, and high throughput. In this study, multiphoton-based in-flow detection of human stem cells in whole, unfiltered blood is demonstrated in a microfluidic channel. The approach relies on a multiphoton microscope with diffractive scanning in the direction perpendicular to the flow via a rapidly wavelength-swept laser. Stem cells are labeled with metal oxide harmonic nanoparticles. Thanks to their strong and quasi-instantaneous second harmonic generation (SHG), an imaging rate in excess of 10 000 frames per second is achieved with pixel dwell times of 1 ns, a duration shorter than typical fluorescence lifetimes yet compatible with SHG. Through automated cell identification and segmentation, morphological features of each individual detected event are extracted and cell aggregates are distinguished from isolated cells. This combination of high-speed multiphoton microscopy and high-sensitivity SHG nanoparticle labeling in turbid media promises the detection of rare cells in the bloodstream for assessing novel cell-based therapies.}
}

@article{Lotz:24,
author = {Simon Lotz and Madita G\"{o}b and Sven B\"{o}ttger and Linh Ha-Wissel and Jennifer Hundt and Floris Ernst and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {Angiography; Biomedical imaging; In vivo imaging; Machine vision; Point clouds; Spectral domain optical coherence tomography},
number = {6},
pages = {3993--4009},
publisher = {Optica Publishing Group},
title = {Large area robotically assisted optical coherence tomography (LARA-OCT)},
volume = {15},
month = {Jun},
year = {2024},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-15-6-3993},
doi = {10.1364/BOE.525524},
abstract = {We demonstrate large-area robotically assisted optical coherence tomography (LARA-OCT), utilizing a seven-degree-of-freedom robotic arm in conjunction with a 3.3\&\#x2005;MHz swept-source OCT to raster scan samples of arbitrary shape. By combining multiple fields of view (FOV), LARA-OCT can probe a much larger area than conventional OCT. Also, nonplanar and curved surfaces like skin on arms and legs can be probed. The lenses in the LARA-OCT scanner with their normal FOV can have fewer aberrations and less complex optics compared to a single wide field design. This may be especially critical for high resolution scans. We directly use our fast MHz-OCT for tracking and stitching, making additional machine vision systems like cameras, positioning, tracking or navigation devices obsolete. This also eliminates the need for complex coordinate system registration between OCT and the machine vision system. We implemented a real time probe-to-surface control that maintains the probe alignment orthogonal to the sample by only using surface information from the OCT images. We present OCT data sets with volume sizes of 140\&\#x2009;\&\#x00D7;\&\#x2009;170\&\#x2009;\&\#x00D7;\&\#x2009;20 mm3, captured in 2.5 minutes.},
}

@article{https://doi.org/10.1111/jdv.20097,
author = {Ha-Wissel, L. and Graßhoff, H. and Göb, M. and Mustafa, B. and Huber, R. and Zirpel, H. and Yasak, H. and Thaçi, D. and Hundt, J. E.},
title = {Optical coherence tomography-based imaging biomarkers for disease activity monitoring in plaque psoriasis},
journal = {Journal of the European Academy of Dermatology and Venereology},
volume = {n/a},
number = {n/a},
pages = {},
doi = {https://doi.org/10.1111/jdv.20097},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/jdv.20097},
eprint = {https://onlinelibrary.wiley.com/doi/pdf/10.1111/jdv.20097}
}

@ARTICLE{10568460,
  author={Riha, Rene and Jimenez, Alejandro Martinez and Venugopal, Gopika and Klufts, Marie and Huber, Robert and Podoleanu, Adrian},
  journal={IEEE Photonics Journal}, 
  title={Dispersion-Tuned Mode-Locked Laser for Swept Source OCT At 850 Nm Using a cFBG and the Pulse Modulation Technique}, 
  year={2024},
  volume={},
  number={},
  pages={1-6},
  keywords={Modulation;Dispersion;Semiconductor optical amplifiers;Optical interferometry;Optical pulses;Frequency modulation;Optical fiber amplifiers;OCT;dispersion tuning;cFBG;intensity modulator},
  doi={10.1109/JPHOT.2024.3417829}}

@article{10.1167/tvst.13.4.26,
    author = {von der Burchard, Claus and Kren, Christopher and Fleger, Jan-Erik and Theisen-Kunde, Dirk and Danicke, Veit and Abbas, Hossam S. and Kleyman, Viktoria and Roider, Johann and Brinkmann, Ralf},
    title = "{Real-Time Temperature-Controlled Retinal Laser Irradiation in Rabbits}",
    journal = {Translational Vision Science & Technology},
    volume = {13},
    number = {4},
    pages = {26-26},
    year = {2024},
    month = {04},
    abstract = "{   Subdamaging thermal retinal laser therapy has the potential to induce regenerative stimuli in retinal diseases, but validated dosimetry is missing. Real-time optoacoustic temperature determination and control could close this gap. This study investigates a first in vivo application.    Two iterations of a control module that were optically coupled in between a continuous-wave commercial laser source and a commercial slit lamp were evaluated on chinchilla rabbits. The module allows extraction of the temperature rise in real time and can control the power of the therapy laser such that a predefined temperature rise at the retina is quickly achieved and held constant. Irradiations with aim temperatures from 45°C to 69°C were performed on a diameter of 200 µm and a heating time of 100 ms.    We analyzed 424 temperature-guided irradiations in nine eyes of five rabbits. The mean difference between the measured and aim temperature was −0.04°C ± 0.98°C. The following ED50 values for visibility thresholds could be determined: 58.6°C for funduscopic visibility, 57.7°C for fluorescein angiography, and 57.0°C for OCT. In all measurements, the correlation of tissue effect was higher to the temperature than to the average heating laser power used.    The system was able to reliably perform temperature-guided irradiations, which allowed for better tissue effect control than simple power control. This approach could enhance the accuracy, safety, and reproducibility of thermal stimulating laser therapy.    This study is a bridge between preclinical ex vivo experiments and a pilot clinical study.  }",
    issn = {2164-2591},
    doi = {10.1167/tvst.13.4.26},
    url = {https://doi.org/10.1167/tvst.13.4.26},
    eprint = {https://arvojournals.org/arvo/content\_public/journal/tvst/938671/i2164-2591-13-4-26\_1713518675.19154.pdf},
}

@article{10.1093/ecco-jcc/jjae034,
    author = {Ellrichmann, Mark and Schulte, Berenice and Conrad, Claudio C and Schoch, Stephan and Bethge, Johannes and Seeger, Marcus and Huber, Robert and Goeb, Madita and Arlt, Alexander and Nikolaus, Susanna and Röcken, Christoph and Schreiber, Stefan},
    title = "{Contrast enhanced endoscopic ultrasound detects early therapy response following anti-TNF-therapy in patients with ulcerative colitis}",
    journal = {Journal of Crohn's and Colitis},
    pages = {jjae034},
    year = {2024},
    month = {03},
    abstract = "{Though colonoscopy plays a crucial role in assessing active ulcerative colitis (aUC), its scope is limited to the mucosal surface. Endoscopic ultrasound (EUS) coupled with contrast-enhancement (dCEUS) can precisely quantify bowel wall thickness and microvascular circulation, potentially enabling the quantitative evaluation of inflammation.We conducted a prospective, longitudinal study to assess therapy response using dCEUS in aUC patients undergoing treatment with adalimumab (ADA) or infliximab (IFX).30 ADA- and 15 IFX-treated aUC patients were examined at baseline and at 2, 6, 14 weeks of therapy and 48 weeks of follow-up. Bowel wall thickness (BWT) was measured by EUS in the rectum. Vascularity was quantified by dCEUS using Rise Time (RT) and Time To Peak (TTP). Therapy response was defined after 14 weeks using the Mayo Score.Patients with aUC displayed a mean BWT of 3.9±0.9 mm. In case of response to ADA/IFX a significant reduction in BWT was observed after 2 weeks (p=0.04), whereas non-responders displayed no significant changes. The TTP was notably accelerated at baseline and significantly normalised by week 2 in responders (p=0.001), while non-responders exhibited no significant alterations (p=0.9). At week 2, the endoscopic Mayo score did not exhibit any changes, thus failing to predict treatment responses.dCEUS enables the early detection of therapy response in patients with aUC, which serves as a predictive marker for long term clinical success. Therefore, dCEUS serves as a diagnostic tool for assessing the probability of future therapy success.}",
    issn = {1873-9946},
    doi = {10.1093/ecco-jcc/jjae034},
    url = {https://doi.org/10.1093/ecco-jcc/jjae034},
    eprint = {https://academic.oup.com/ecco-jcc/advance-article-pdf/doi/10.1093/ecco-jcc/jjae034/56911128/jjae034.pdf},
}

@article{RN5474,
   author = {Schulte, Berenice;Göb, Madita;Singh, Awanish Pratap;Lotz, Simon;Draxinger, Wolfgang;Heimke, Marvin;pieper, Mario;Heinze, Tillmann;Wedel, Thilo;Rahlves, Maik;Huber, Robert and Ellrichmann, Mark},
   title = {High-resolution rectoscopy using MHz optical coherence tomography: a step towards real time 3D endoscopy},
   journal = {Scientific Reports},
   volume = {14},
   number = {1},
   pages = {4672},
   ISSN = {2045-2322},
   DOI = {10.1038/s41598-024-55338-5},
   url = {https://doi.org/10.1038/s41598-024-55338-5},
   year = {2024},
   type = {Journal Article}
}

@article{RN5472,
   author = {Kren, Jessica;Skambath, Isabelle;Kuppler, Patrick;Buschschlüter, Steffen;Detrez, Nicolas;Burhan, Sazgar;Huber, Robert;Brinkmann, Ralf and Bonsanto, Matteo Mario},
   title = {Mechanical characteristics of glioblastoma and peritumoral tumor-free human brain tissue},
   journal = {Acta Neurochirurgica},
   volume = {166},
   number = {1},
   pages = {102},
   ISSN = {0942-0940},
   DOI = {10.1007/s00701-024-06009-x},
   url = {https://doi.org/10.1007/s00701-024-06009-x},
   year = {2024},
   type = {Journal Article}
}

@article{10.1159/000535930,
    author = {Lange, Clemens A. and Ohlmeier, Charlotte and Kiskämper, Anna and von Schwarzkopf, Christoph and Hufnagel, Hinrich and Gruber, Markus and Schworm, Benedikt and Brocks, Ulrike and Reinking, Franziska and Schreiner, Lisa and Miura, Yoko and Grundel, Milena and Lohmann, Tibor and Clemens, Christoph R. and Gamulescu, Maria-Andreea and Eter, Nicole and Grisanti, Salvatore and Priglinger, Siegfried and Spitzer, Martin S. and Walter, Peter and Agostini, Hansjürgen A. and Stahl, Andreas and Pauleikhoff, Laurenz J.B. and for the Retina.net CSC-Registry-Study Group},
    title = "{Clinical Landscape of Central Serous Chorioretinopathy in Germany: Retina.net CSC Registry Report Number 1}",
    journal = {Ophthalmologica},
    pages = {1-12},
    year = {2024},
    month = {02},
    abstract = "{Introduction: The German Registry of central serous chorioretinopathy (CSC) collects data on CSC patients in a nationwide multicenter approach to analyze epidemiology, risk factors, clinical presentations, as well as diagnosis and treatment patterns. Methods: In this multicenter cohort study, patients with CSC were enrolled in nine tertiary referral centers in Germany between January 2022 and June 2023. After consenting to the study, demographic data, risk factors, reported symptoms, best-corrected visual acuity (BCVA), funduscopic findings, disease severity, and diagnostic and treatment decisions were recorded and analyzed. Results: A total of 539 eyes of 411 CSC patients were enrolled in this study including 308 males (75\\%) and 103 females (25\\%). Patients were predominantly of Caucasian origin and had a mean age of 55.5 years (IQR 41.0–70.0). 28\\% of eyes were classified as acute (\\<4 months duration) CSC, 28\\% as chronic (\\>4 months duration) CSC, 21\\% as inactive CSC, 11\\% as chronic atrophic CSC, and 12\\% as CSC with secondary CNV. 128 patients (31\\%) demonstrated bilateral CSC. The most common risk factors reported were psychological stress (52\\%), smoking (38\\%), arterial hypertension (38\\%), and a history of or current use of steroids (30\\%). Most frequently encountered symptoms included decreased visual acuity (76\\%), metamorphopsia (49\\%), relative scotoma (47\\%), blurred vision (19\\%), and dyschromatopsia (9\\%). The mean logMAR BCVA on initial examination was 0.2 (≈20/30, IQR 0.2–0.4) but showed significant variation with a tendency of lower BCVA in chronic cases. At the baseline visit, 74\\% of the overall cohort received no treatment, while 19\\% underwent local treatment and only 2\\% underwent systemic treatment. Of the local therapies, anti-VEGF injections were the most frequently performed procedure (33\\%, mainly for secondary CNV), followed by micropulse laser (28\\%), focal nonpulsed laser (23\\%), verteporfin photodynamic therapy (14\\%), and nonsteroidal anti-inflammatory eye drops (2\\%). Among intravitreal anti-VEGF agents, aflibercept was used most frequently, followed by bevacizumab and ranibizumab. Conclusion: This registry represents one of the largest cohorts of European patients with CSC to date. Patient age and the proportion of women were higher than expected and bilateral active disease was lower than anticipated, highlighting that neither age nor gender should be overemphasized when diagnosing CSC. Therapeutic interventions are heterogeneous and include verteporfin photodynamic therapy, micropulse laser, and anti-VEGF injections in case of secondary CNV. }",
    issn = {0030-3755},
    doi = {10.1159/000535930},
    url = {https://doi.org/10.1159/000535930},
    eprint = {https://karger.com/oph/article-pdf/doi/10.1159/000535930/4178133/000535930.pdf},
}

@article{10.1167/tvst.13.1.24,
    author = {Sonntag, Svenja Rebecca and Klein, Britta and Brinkmann, Ralf and Grisanti, Salvatore and Miura, Yoko},
    title = "{Fluorescence Lifetime Imaging Ophthalmoscopy of Mouse Models of Age-related Macular Degeneration}",
    journal = {Translational Vision Science & Technology},
    volume = {13},
    number = {1},
    pages = {24-24},
    year = {2024},
    month = {01},
    abstract = "{   To investigate fluorescence lifetime of mouse models of age-related macular degeneration (AMD) by fluorescence lifetime imaging ophthalmoscopy (FLIO).    Two AMD mouse models, apolipoprotein E knockout (ApoE−/−) mice and NF-E2-related factor-2 knockout (Nrf2−/−) mice, and their wild-type mice underwent monthly ophthalmic examinations including FLIO from 3 months of age. After euthanasia at the age of 6 or 11 months, blood plasma was collected to determine total antioxidant capacity and eyes were enucleated for Oil red O (ORO) lipid staining of chorioretinal tissue.    In FLIO, the mean fluorescence lifetime (τm) of wild type shortened with age in both spectral channels. In short spectral channel, τm shortening was observed in both AMD models as well, but its rate was more pronounced in ApoE−/− mice and significantly different from the other strains as months of age progressed. In contrast, in long spectral channel, both model strains showed completely opposite trends, with τm becoming shorter in ApoE−/− and longer in Nrf2−/− mice than the others. Oil red O staining at Bruch's membrane was significantly stronger in ApoE−/− mice at 11 months than the other strains. Plasma total antioxidant capacity was highest in ApoE−/− mice at both 6 and 11 months.    The two AMD mouse models exhibited largely different fundus fluorescence lifetime, which might be related to the different systemic metabolic state. FLIO might be able to indicate different metabolic states of eyes at risk for AMD.    This animal study may provide new insights into the relationship between early AMD-associated metabolic changes and FLIO findings.  }",
    issn = {2164-2591},
    doi = {10.1167/tvst.13.1.24},
    url = {https://doi.org/10.1167/tvst.13.1.24},
    eprint = {https://arvojournals.org/arvo/content\_public/journal/tvst/938660/i2164-2591-13-1-24\_1706520239.75643.pdf},
}

@Article{Schulte2024,
author={Schulte, B.; Burhan, S.; Singh, A. P.; Draxinger, W.; Lotz, S.; Heimke, M.; Heinze, T.; Wedel, T.; Rahlves, M.; Huber, R.; Ellrichmann, M.},
title={Hochaufl{\"o}sende Rektoskopie mittels dual-mode MHz optischer Koh{\"a}renztomographie -- ein Schritt zur real time 3D Endoskopie},
journal={Z Gastroenterol},
year={2024},
month={Sep},
day={26},
publisher={Georg Thieme Verlag KG},
volume={62},
number={09},
pages={KV 355},
issn={0044-2771},
doi={10.1055/s-0044-1790019},
url={http://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0044-1790019},
url={https://doi.org/10.1055/s-0044-1790019},
language={DE}
}

@article{doi:10.1021/acs.molpharmaceut.3c00749,
author = {Enzian, Paula and Kleineberg, Nina and Kirchert, Elisabeth and Schell, Christian and Rahmanzadeh, Ramtin},
title = {Light-Induced Liposomal Drug Delivery with an Amphiphilic Porphyrin and Its Chlorin and Bacteriochlorin Analogues},
journal = {Molecular Pharmaceutics},
volume = {0},
number = {0},
pages = {null},
year = {0},
doi = {10.1021/acs.molpharmaceut.3c00749},
    note ={PMID: 38189667},

URL = { 
    
        https://doi.org/10.1021/acs.molpharmaceut.3c00749
    
    

},
eprint = { 
    
        https://doi.org/10.1021/acs.molpharmaceut.3c00749
    
    

}

}

@Article{diagnostics14040431,
AUTHOR = {Thiemann, Natalie and Sonntag, Svenja Rebecca and Kreikenbohm, Marie and Böhmerle, Giulia and Stagge, Jessica and Grisanti, Salvatore and Martinetz, Thomas and Miura, Yoko},
TITLE = {Artificial Intelligence in Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) Data Analysis—Toward Retinal Metabolic Diagnostics},
JOURNAL = {Diagnostics},
VOLUME = {14},
YEAR = {2024},
NUMBER = {4},
ARTICLE-NUMBER = {431},
URL = {https://www.mdpi.com/2075-4418/14/4/431},
ISSN = {2075-4418},
ABSTRACT = {The purpose of this study was to investigate the possibility of implementing an artificial intelligence (AI) approach for the analysis of fluorescence lifetime imaging ophthalmoscopy (FLIO) data even with small data. FLIO data, including the fluorescence intensity and mean fluorescence lifetime (τm) of two spectral channels, as well as OCT-A data from 26 non-smokers and 28 smokers without systemic and ocular diseases were used. The analysis was performed with support vector machines (SVMs), a well-known AI method for small datasets, and compared with the results of convolutional neural networks (CNNs) and autoencoder networks. The SVM was the only tested AI method, which was able to distinguish τm between non-smokers and heavy smokers. The accuracy was about 80%. OCT-A data did not show significant differences. The feasibility and usefulness of the AI in analyzing FLIO and OCT-A data without any apparent retinal diseases were demonstrated. Although further studies with larger datasets are necessary to validate the results, the results greatly suggest that AI could be useful in analyzing FLIO-data even from healthy subjects without retinal disease and even with small datasets. AI-assisted FLIO is expected to greatly advance early retinal diagnosis.},
DOI = {10.3390/diagnostics14040431}
}

@ARTICLE{10.3389/fopht.2024.1340692,
  
AUTHOR={Pfäffle, Clara and Puyo, Léo and Spahr, Hendrik and Hillmann, Dierck and Miura, Yoko and Hüttmann, Gereon},   
	 
TITLE={Unraveling the Functional Signals of Rods and Cones in the Human Retina: Separation and Analysis},      
	
JOURNAL={Frontiers in Ophthalmology},      
	
VOLUME={4},           
	
YEAR={2024},      
	  
URL={https://www.frontiersin.org/articles/10.3389/fopht.2024.1340692},       
	
DOI={10.3389/fopht.2024.1340692},      
	
ISSN={2674-0826}   
   
}

@article{Singh:24,
author = {Awanish Pratap Singh and Madita G\"{o}b and Martin Ahrens and Tim Eixmann and Berenice Schulte and Hinnerk Schulz-Hildebrandt and Gereon H\"{u}ttmann and Mark Ellrichmann and Robert Huber and Maik Rahlves},
journal = {Opt. Express},
keywords = {Biomedical imaging; Endoscopic imaging; Imaging systems; Optical coherence tomography; Real time imaging; Vertical cavity surface emitting lasers},
number = {4},
pages = {5809--5825},
publisher = {Optica Publishing Group},
title = {Virtual Hall sensor triggered multi-MHz endoscopic OCT imaging for stable real-time visualization},
volume = {32},
month = {Feb},
year = {2024},
url = {https://opg.optica.org/oe/abstract.cfm?URI=oe-32-4-5809},
doi = {10.1364/OE.514636},
abstract = {Circumferential scanning in endoscopic imaging is crucial across various disciplines, and optical coherence tomography (OCT) is often the preferred choice due to its high-speed, high-resolution, and micron-scale imaging capabilities. Moreover, real-time and high-speed 3D endoscopy is a pivotal technology for medical screening and precise surgical guidance, among other applications. However, challenges such as image jitter and non-uniform rotational distortion (NURD) are persistent obstacles that hinder real-time visualization during high-speed OCT procedures. To address this issue, we developed an innovative, low-cost endoscope that employs a brushless DC motor for scanning, and a sensorless technique for triggering and synchronizing OCT imaging with the scanning motor. This sensorless approach uses the motor\&\#x2019;s electrical feedback (back electromotive force, BEMF) as a virtual Hall sensor to initiate OCT image acquisition and synchronize it with a Fourier Domain Mode-Locked (FDML)-based Megahertz OCT system. Notably, the implementation of BEMF-triggered OCT has led to a substantial reduction in image jitter and NURD (\<4 mrad), thereby opening up a new window for real-time visualization capabilities. This approach suggests potential benefits across various applications, aiming to provide a more accurate, deployable, and cost-effective solution. Subsequent studies can explore the adaptability of this system to specific clinical scenarios and its performance under practical endoscopic conditions.},
}

@Article{s24072105,
AUTHOR = {Boyko, Andrey and Lange, Birgit and Eckert, Sebastian and Mayorov, Fedor and Brinkmann, Ralf},
TITLE = {Signal Enhancement of a Differential Photoacoustic Cell by Connecting the Microphones via Capillaries},
JOURNAL = {Sensors},
VOLUME = {24},
YEAR = {2024},
NUMBER = {7},
ARTICLE-NUMBER = {2105},
URL = {https://www.mdpi.com/1424-8220/24/7/2105},
ISSN = {1424-8220},
ABSTRACT = {Differential photoacoustic spectroscopy (DPAS) cells are usually excited on the first longitudinal ring mode, with a microphone situated in the middle of each of the two resonator tubes. However, it is known from other photoacoustic spectroscopy cell designs that connecting the microphones via a capillary can lead to signal enhancement. By means of finite element method (FEM) simulations, we compared such a photoacoustic spectroscopy (PAS) cell with a capillary to a DPAS cell with a capillary attached to each of the two resonators and showed that the behavior of both systems is qualitatively the same: In both the PAS and the DPAS cell, in-phase and anti-phase oscillations of the coupled system (resonator–capillary) can be excited. In the DPAS cell, capillaries of suitable length also increase the pressure signal at the microphones according to the FEM simulations. For different capillary diameters (1.2 mm/1.7 mm/2.2 mm), the respective optimal capillary length (36–37.5 mm) and signal amplification was determined (94%, 70%, 53%). According to the results of these FEM simulations, a significant increase in sensitivity can, therefore, also be achieved in DPAS cells by expanding them with thin tubes leading to the microphones.},
DOI = {10.3390/s24072105}
}

@article{Dolling:24,
author = {Maron Dolling and Lara Buhl and Reginald Birngruber and Gereon H\"{u}ttmann and Hinnerk Schulz-Hildebrandt},
journal = {Appl. Opt.},
keywords = {Distortion; Imaging systems; Optical aberration; Optical components; Spectral domain optical coherence tomography; Systems design},
number = {10},
pages = {2694--2703},
publisher = {Optica Publishing Group},
title = {Algorithm and software for field distortion correction in a commercial SD-OCT for corneal curvature assessment},
volume = {63},
month = {Apr},
year = {2024},
url = {https://opg.optica.org/ao/abstract.cfm?URI=ao-63-10-2694},
doi = {10.1364/AO.505107},
abstract = {Accurate assessment of corneal curvatures using frequency domain optical coherence tomography (OCT) with galvanometer scanners remains challenging due to the well-known scan field distortion. This paper presents an algorithm and software for correcting the distortion using only two simple measurements in which a readily available standard sphere is positioned in different depths in front of the OCT scanner. This offers a highly accessible and easily reproducible method for the field distortion correction (FDC). The correction was validated by measuring different spherical phantoms and conducting corneal curvature measurements of ex vivo porcine corneas using a commercial spectral-domain OCT system and a clinically approved swept-source OCT as a reference instrument. Thus, the error in radius measurements of spherical phantoms was reduced by \>90\% and astigmatism by \>80\% using FDC. In explanted porcine eyes, the error in astigmatism measurements with the Telesto was reduced by 75\% for power and 70\% for angle. The best fitting sphere radius was determined up to a deviation of 0.4\% from the Anterion. This paper describes a correction algorithm for OCT immanent distortion that is applicable to any scanning OCT setup and enables precise corneal curvature measurements. The MATLAB software for the FDC is publicly available on GitHub.},
}

@article{Fu:24,
author = {Lei Fu and Jing Wang and Siqi Wang and Zhenxi Zhang and Alfred Vogel and Xiao-xuan Liang and Cuiping Yao},
journal = {Opt. Express},
keywords = {Laser beams; Laser light; Laser materials; Laser surgery; Numerical simulation; Phase shift},
number = {6},
pages = {9747--9766},
publisher = {Optica Publishing Group},
title = {Secondary cavitation bubble dynamics during laser-induced bubble formation in a small container},
volume = {32},
month = {Mar},
year = {2024},
url = {https://opg.optica.org/oe/abstract.cfm?URI=oe-32-6-9747},
doi = {10.1364/OE.516264},
abstract = {We investigated secondary cavitation bubble dynamics during laser-induced bubble formation in a small container with a partially confined free surface and elastic thin walls. We employed high-speed photography to record the dynamics of sub-mm-sized laser-induced bubbles and small secondary bubble clouds. Simultaneous light scattering and acoustic measurements were used to detect the oscillation times of laser-induced bubbles. We observed that the appearance of secondary bubbles coincides with a prolonged collapse phase and with re-oscillations of the laser-induced bubble. We observed an asymmetric distribution of secondary bubbles with a preference for the upstream side of the focus, an absence of secondary bubbles in the immediate vicinity of the laser focus, and a migration of laser-induced bubble toward secondary bubbles at large pulse energies. We found that secondary bubbles are created through heating of impurities to form initial nanobubble nuclei, which are further expanded by rarefaction waves. The rarefaction waves originate from the vibration of the elastic thin walls, which are excited either directly by laser-induced bubble or by bubble-excited liquid-mass oscillations. The oscillation period of thin walls and liquid-mass were Twall\&\#x2009;\&\#x003D;\&\#x2009;116 \&\#x00B5;s and Tlm \&\#x2248; 160 \&\#x00B5;s, respectively. While the amplitude of the wall vibrations increases monotonically with the size of laser-induced bubbles, the amplitude of liquid-mass oscillation undulates with increasing bubble size. This can be attributed to a phase shift between the laser-induced bubble oscillation and the liquid-mass oscillator. Mutual interactions between the laser-induced bubble and secondary bubbles reveal a fast-changing pressure gradient in the liquid. Our study provides a better understanding of laser-induced bubble dynamics in a partially confined environment, which is of practical importance for microfluidics and intraluminal laser surgery.},
}

@article{https://doi.org/10.1002/lsm.23833,
author = {von der Burchard, Claus and Miura, Yoko and Stanzel, Boris and Chhablani, Jay and Roider, Johann and Framme, Carsten and Brinkmann, Ralf and Tode, Jan},
title = {Regenerative Retinal Laser and Light Therapies (RELITE): Proposal of a New Nomenclature, Categorization, and Trial Reporting Standard},
journal = {Lasers in Surgery and Medicine},
volume = {56},
number = {8},
pages = {693-708},
keywords = {nomenclature, retinal laser therapy, subvisible laser therapy},
doi = {https://doi.org/10.1002/lsm.23833},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/lsm.23833},
eprint = {https://onlinelibrary.wiley.com/doi/pdf/10.1002/lsm.23833},
abstract = {ABSTRACT Objectives Numerous laser and light therapies have been developed to induce regenerative processes in the choroid/retinal pigment epithelium (RPE)/photoreceptor complex, leaving the neuroretina undamaged. These therapies are applied to the macula for the treatment of various diseases, most prominently diabetic maculopathy, retinal vein occlusion, central serous chorioretinopathy, and age-related macular degeneration. However, the abundance of technologies, treatment patterns, and dosimetry protocols has made understanding these therapies and comparing different approaches increasingly complex and challenging. To address this, we propose a new nomenclature system with a clear categorization that will allow for better understanding and comparability between different laser and light modalities. We propose this nomenclature system as an open standard that may be adapted in future toward new technical developments or medical advancements. Methods A systematic literature review of reported macular laser and light therapies was conducted. A categorization into a standardized system was proposed and discussed among experts and professionals in the field. This paper does not aim to assess, compare, or evaluate the efficacy of different laser or dosimetry techniques or treatment patterns. Results The literature search yielded 194 papers describing laser techniques, 50 studies describing dosimetry, 272 studies with relevant clinical trials, and 82 reviews. Following the common therapeutic aim, we propose “regenerative retinal laser and light therapies (RELITE)” as the general header. We subdivided RELITE into four main categories that refer to the intended physical and biochemical effects of temperature increase (photothermal therapy, PTT), RPE regeneration (photomicrodisruption therapy, PMT), photochemical processes (photochemical therapy, PCT), and photobiomodulation (photobiomodulation therapy, PBT). Further, we categorized the different dosimetry approaches and treatment regimens. We propose the following nomenclature system that integrates the most important parameters to enable understanding and comparability: Pattern—Dosimetry—Exposure Time/Frequency, Duty Cycle/Irradiation Diameter/Wavelength—Subcategory—Category. Conclusion Regenerative retinal laser and light therapies are widely used for different diseases and may become valuable in the future. A precise nomenclature system and strict reporting standards are needed to allow for a better understanding, reproduceable and comparable clinical trials, and overall acceptance. We defined categories for a systematic therapeutic goal-based nomenclature to facilitate future research in this field.},
year = {2024}
}

@article{Burhan:24,
author = {Sazgar Burhan and Nicolas Detrez and Katharina Rewerts and Paul Strenge and Steffen Buschschl\"{u}ter and Jessica Kren and Christian Hagel and Matteo Mario Bonsanto and Ralf Brinkmann and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {High speed imaging; Imaging systems; In vivo imaging; Magnetic resonance imaging; Phase noise; Phase shift},
number = {2},
pages = {1038--1058},
publisher = {Optica Publishing Group},
title = {Phase unwrapping for MHz optical coherence elastography and application to brain tumor tissue},
volume = {15},
month = {Feb},
year = {2024},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-15-2-1038},
doi = {10.1364/BOE.510020},
abstract = {During neuro-oncologic surgery, phase-sensitive optical coherence elastography (OCE) can be valuable for distinguishing between healthy and diseased tissue. However, the phase unwrapping process required to retrieve the original phase signal is a challenging and critical task. To address this issue, we demonstrate a one-dimensional unwrapping algorithm that recovers the phase signal from a 3.2\&\#x2005;MHz OCE system. With a processing time of approximately 0.11 s per frame on the GPU, multiple 2\&\#x03C0; wraps are detected and corrected. By utilizing this approach, exact and reproducible information on tissue deformation can be obtained with pixel accuracy over the entire acquisition time. Measurements of brain tumor-mimicking phantoms and human ex vivo brain tumor samples verified the algorithm\&\#x0027;s reliability. The tissue samples were subjected to a 200\&\#x2005;ms short air pulse. A correlation with histological findings confirmed the algorithm\&\#x0027;s dependability.},
}

@article { Microscopeintegratedopticalcoherencetomographyforinvivohumanbraintumordetectionwithartificialintelligence,
      author = "Patrick Kuppler and Paul Strenge and Birgit Lange and Sonja Spahr-Hess and Wolfgang Draxinger and Christian Hagel and Dirk Theisen-Kunde and Ralf Brinkmann and Robert Huber and Volker Tronnier and Matteo Mario Bonsanto",
      title = "Microscope-integrated optical coherence tomography for in vivo human brain tumor detection with artificial intelligence",
      journal = "Journal of Neurosurgery",
      year = "2024",
      publisher = "American Association of Neurological Surgeons",
      doi = "10.3171/2024.1.JNS231511",
      pages=      "1 - 9",
      url = "https://thejns.org/view/journals/j-neurosurg/aop/article-10.3171-2024.1.JNS231511/article-10.3171-2024.1.JNS231511.xml"
}

@article{RN5490,
   author = {Kajita, Keisuke;Nishida, Mitsuhiro;Kurimoto, Yasuo;Yokota, Satoshi;Sugita, Sunao;Semba, Toshika;Shirae, Satoshi;Hayashi, Naoko;Ozaki, Atsuta;Miura, Yoko;Maeda, Akiko;Mitamura, Yoshinori;Takahashi, Masayo and Mandai, Michiko},
   title = {Graft cell expansion from hiPSC-RPE strip after transplantation in primate eyes with or without RPE damage},
   journal = {Scientific Reports},
   volume = {14},
   number = {1},
   pages = {10044},
   ISSN = {2045-2322},
   DOI = {10.1038/s41598-024-60895-w},
   url = {https://doi.org/10.1038/s41598-024-60895-w},
   year = {2024},
   type = {Journal Article}
}

@article{RN5480,
   author = {Sonntag, Svenja Rebecca;Hamann, Maximilian;Seifert, Eric;Grisanti, Salvatore;Brinkmann, Ralf and Miura, Yoko},
   title = {Detection sensitivity of fluorescence lifetime imaging ophthalmoscopy for laser-induced selective damage of retinal pigment epithelium},
   journal = {Graefe's Archive for Clinical and Experimental Ophthalmology},
   ISSN = {1435-702X},
   DOI = {10.1007/s00417-024-06449-2},
   url = {https://doi.org/10.1007/s00417-024-06449-2},
   year = {2024},
   type = {Journal Article}
}

@article{Klufts:23,
author = {Marie Klufts and Alejandro Martinez Jimenez and Simon Lotz and Muhammad Asim Bashir and Tom Pfeiffer and Alexander Mlynek and Wolfgang Wieser and Alexander Chamorovskiy and Adrian Bradu and Adrian Podoleanu and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {Analog to digital converters; Laser beams; Laser imaging; Laser modes; Point spread function; Vertical cavity surface emitting lasers},
number = {12},
pages = {6493--6508},
publisher = {Optica Publishing Group},
title = {828 kHz retinal imaging with an 840\&\#x2005;nm Fourier domain mode locked laser},
volume = {14},
month = {Dec},
year = {2023},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-14-12-6493},
abstract = {This paper presents a Fourier domain mode locked (FDML) laser centered around 840 nm. It features a bidirectional sweep repetition rate of 828 kHz and a spectral bandwidth of 40 nm. An axial resolution of ∼9.9 µm in water and a 1.4 cm sensitivity roll-off are achieved. Utilizing a complex master-slave (CMS) recalibration method and due to a sufficiently high sensitivity of 84.6 dB, retinal layers of the human eye in-vivo can be resolved during optical coherence tomography (OCT) examination. The developed FDML laser enables acquisition rates of 3D-volumes with a size of 200 × 100 × 256 voxels in under 100 milliseconds. Detailed information on the FDML implementation, its challenging design tasks, and OCT images obtained with the laser are presented in this paper.},
}

@Article{pharmaceutics15082040,
AUTHOR = {Enzian, Paula and Rahmanzadeh, Ramtin},
TITLE = {Photochemical Internalization with Fimaporfin: Enhanced Bleomycin Treatment for Head and Neck Cancer},
JOURNAL = {Pharmaceutics},
VOLUME = {15},
YEAR = {2023},
NUMBER = {8},
ARTICLE-NUMBER = {2040},
URL = {https://www.mdpi.com/1999-4923/15/8/2040},
ISSN = {1999-4923},
ABSTRACT = {Head and neck squamous cell carcinoma (HNSCC) still represents the world’s sixth most common tumor entity, with increasing incidence. The reachability of light makes HNSCC suitable for light-based therapies such as Photochemical Internalization (PCI). The drug Bleomycin is cytotoxic and used as an anti-tumor medication. Since Bleomycin is endocytosed as a relatively large molecule, part of it is degraded in lysosomes before reaching its intracellular target. The goal of our study was to improve the intracellular availability of Bleomycin with PCI. We investigate the intracellular delivery of Bleomycin after PCI with the photosensitizer Fimaporfin. A systematic variation of Bleomycin and Fimaporfin concentrations and light irradiation led to the pronounced cell death of HNSCC cells. After optimization, the same level of tumor cell death of 75% was reached with a 20-fold lower Bleomycin concentration. This would allow treatment of HNSCC with high local tumor cell death and reduce the side effects of Bleomycin, e.g., lung fibrosis, at the same time. This demonstrates the increased efficacy of the anti-tumor medication Bleomycin in combination with PCI.},
DOI = {10.3390/pharmaceutics15082040}
}

@article{RN5446,
   author = {Sonntag, Svenja Rebecca;Kreikenbohm, Marie;Böhmerle, Giulia;Stagge, Jessica;Grisanti, Salvatore and Miura, Yoko},
   title = {Impact of cigarette smoking on fluorescence lifetime of ocular fundus},
   journal = {Scientific Reports},
   volume = {13},
   number = {1},
   pages = {11484},
   ISSN = {2045-2322},
   DOI = {10.1038/s41598-023-37484-4},
   url = {https://doi.org/10.1038/s41598-023-37484-4},
   year = {2023},
   type = {Journal Article}
}

@article{Lamminger:23,
author = {Philipp Lamminger and Hubertus Hakert and Simon Lotz and Jan Philip Kolb and Tonio Kutscher and Sebastian Karpf and Robert Huber},
journal = {Opt. Lett.},
keywords = {Green fluorescent protein; Laser beam combining; Laser crystals; Laser imaging; Optical amplifiers; Photonic crystal lasers},
number = {14},
pages = {3713--3716},
publisher = {Optica Publishing Group},
title = {Four-wave mixing seeded by a rapid wavelength-sweeping FDML laser for nonlinear imaging at 900 nm and 1300 nm},
volume = {48},
month = {Jul},
year = {2023},
url = {https://opg.optica.org/ol/abstract.cfm?URI=ol-48-14-3713},
doi = {10.1364/OL.488181},
abstract = {Four-wave mixing (FWM) enables the generation and amplification of light in spectral regions where suitable fiber gain media are unavailable. The 1300 nm and 900 nm regions are of especially high interest for time-encoded (TICO) stimulated Raman scattering microscopy and spectro-temporal laser imaging by diffracted excitation (SLIDE) two-photon microscopy. We present a new, to the best of our knowledge, FWM setup where we shift the power of a home-built fully fiber-based master oscillator power amplifier (MOPA) at 1064 nm to the 1300-nm region of a rapidly wavelength-sweeping Fourier domain mode-locked (FDML) laser in a photonic crystal fiber (PCF) creating pulses in the 900-nm region. The resulting 900-nm light can be wavelength swept over 54 nm and has up to 2.5 kW (0.2 {\textmu}J) peak power and a narrow instantaneous spectral linewidth of 70 pm. The arbitrary pulse patterns of the MOPA and the fast wavelength tuning of the FDML laser (419 kHz) allow it to rapidly tune the FWM light enabling new and faster TICO-Raman microscopy, SLIDE imaging, and other applications.},
}

@Article{photonics10060685,
AUTHOR = {Leichtle, Anke and Penxova, Zuzana and Kempin, Thorge and Leffers, David and Ahrens, Martin and König, Peter and Brinkmann, Ralf and Hüttmann, Gereon and Bruchhage, Karl-Ludwig and Schulz-Hildebrandt, Hinnerk},
TITLE = {Dynamic Microscopic Optical Coherence Tomography as a New Diagnostic Tool for Otitis Media},
JOURNAL = {Photonics},
VOLUME = {10},
YEAR = {2023},
NUMBER = {6},
ARTICLE-NUMBER = {685},
URL = {https://www.mdpi.com/2304-6732/10/6/685},
ISSN = {2304-6732},
ABSTRACT = {Hypothesis: Otitis media (OM) can be successfully visualized and diagnosed by dynamic microscopic optical coherence tomography (dmOCT). Background: OM is one of the most common infectious diseases and, according to the WHO, one of the leading health problems with high mortality in developing countries. Despite intensive research, the only definitive treatment of therapy-refractory OM for decades has been the surgical removal of inflamed tissue. Thereby, the intra-operative diagnosis is limited to the surgeon’s visual impression. Supportive imaging modalities have been little explored and have not found their way into clinical application. Finding imaging techniques capable of identifying inflamed tissue intraoperatively, therefore, is of significant clinical relevance. Methods: This work investigated a modified version of optical coherence tomography with a microscopic resolution (mOCT) regarding its ability to differentiate between healthy and inflamed tissue. Despite its high resolution, the differentiation of single cells with mOCT is often impossible. A new form of mOCT termed dynamic mOCT (dmOCT) achieves cellular contrast using micro-movements within cells based on their metabolism. It was used in this study to establish correlative measurements with histology. Results: Using dmOCT, images with microscopic resolution were acquired on ex vivo tissue samples of chronic otitis media and cholesteatoma. Imaging with dmOCT allowed the visualization of specific and characteristic cellular and subcellular structures in the cross-sectional images, which can be identified only to a limited extent in native mOCT. Conclusion: We demonstrated for the first time a new marker-free visualization in otitis media based on intracellular motion using dmOCT.},
DOI = {10.3390/photonics10060685}
}

@article{
doi:10.1073/pnas.2220132120,
author = {Michael Schmalz  and Xiao-Xuan Liang  and Ines Wieser  and Caroline Gruschel  and Lukas Muskalla  and Martin Thomas Stöckl  and Roland Nitschke  and Norbert Linz  and Alfred Leitenstorfer  and Alfred Vogel  and Elisa Ferrando-May },
title = {Dissection of DNA damage and repair pathways in live cells by femtosecond laser microirradiation and free-electron modeling},
journal = {Proceedings of the National Academy of Sciences},
volume = {120},
number = {25},
pages = {e2220132120},
year = {2023},
doi = {10.1073/pnas.2220132120},
URL = {https://www.pnas.org/doi/abs/10.1073/pnas.2220132120},
eprint = {https://www.pnas.org/doi/pdf/10.1073/pnas.2220132120},
abstract = {Understanding and predicting the outcome of the interaction of light with DNA has a significant impact on the study of DNA repair and radiotherapy. We report on a combination of femtosecond pulsed laser microirradiation at different wavelengths, quantitative imaging, and numerical modeling that yields a comprehensive picture of photon-mediated and free-electron-mediated DNA damage pathways in live cells. Laser irradiation was performed under highly standardized conditions at four wavelengths between 515 nm and 1,030 nm, enabling to study two-photon photochemical and free-electron-mediated DNA damage in situ. We quantitatively assessed cyclobutane pyrimidine dimer (CPD) and γH2AX-specific immunofluorescence signals to calibrate the damage threshold dose at these wavelengths and performed a comparative analysis of the recruitment of DNA repair factors xeroderma pigmentosum complementation group C (XPC) and Nijmegen breakage syndrome 1 (Nbs1). Our results show that two-photon-induced photochemical CPD generation dominates at 515 nm, while electron-mediated damage dominates at wavelengths ≥620 nm. The recruitment analysis revealed a cross talk between nucleotide excision and homologous recombination DNA repair pathways at 515 nm. Numerical simulations predicted electron densities and electron energy spectra, which govern the yield functions of a variety of direct electron-mediated DNA damage pathways and of indirect damage by •OH radicals resulting from laser and electron interactions with water. Combining these data with information on free electron–DNA interactions gained in artificial systems, we provide a conceptual framework for the interpretation of the wavelength dependence of laser-induced DNA damage that may guide the selection of irradiation parameters in studies and applications that require the selective induction of DNA lesions.}}

@article{ALTMANN2023101105,
title = {Porphyrin-based sensor films for monitoring food spoilage},
journal = {Food Packaging and Shelf Life},
volume = {38},
pages = {101105},
year = {2023},
issn = {2214-2894},
doi = {https://doi.org/10.1016/j.fpsl.2023.101105},
url = {https://www.sciencedirect.com/science/article/pii/S2214289423000820},
author = {Alexander Altmann and Michel Eden and Gereon Hüttmann and Christian Schell and Ramtin Rahmanzadeh},
keywords = {Biogenic amines, Sensors, Fish freshness, Food safety, Porphyrin, Smart packaging},
abstract = {To increase food safety and to minimize food waste, it is interesting for the food industry and consumers to be able to determine food spoilage continuously and non-destructively. When food of animal origin is degraded, amines are released as protein breakdown products, which could be used to monitor the freshness of meat and fish. In this work, we introduce a porphyrin-based sensor foil aimed at the detection of biogenic amines. The sensor-porphyrin is formulated on mesoporous silica. Reactivity towards moderate humidity was eliminated by dispersion of the functionalized silica in polyethylene (PE), followed by thermal extrusion resulting in PE foils. After exposure to amines, the sensor foil changes its color irreversibly from green to red. The color change is accompanied by a pronounced shift of the fluorescence spectrum, which was used as a sensitive method to detect the degradation of fish products in model experiments. Titanium dioxide particles in the foil increased the detected fluorescence emission. Experiments with fish filets showed the applicability of the sensor foils in a real-life application by indicating the degree of spoilage after several days, while the microbial growth was depicted by total viable count. We anticipate that our sensor can be an integral part of smart food packages, helping to track the freshness of food during transport or storage.}
}

@ARTICLE{10122996,

  author={Latus, Sarah and Grube, Sarah and Eixmann, Tim and Neidhardt, Maximilian and Gerlach, Stefan and Mieling, Robin and Hüttmann, Gereon and Lutz, Matthias and Schlaefer, Alexander},

  journal={IEEE Transactions on Biomedical Engineering}, 

  title={A Miniature Dual-Fiber Probe for Quantitative Optical Coherence Elastography}, 

  year={2023},

  volume={},

  number={},

  pages={1-9},

  doi={10.1109/TBME.2023.3275539}}

Kuppler P, Strenge P, Lange B, Spahr-Hess S, Draxinger W, Hagel C, Theisen-Kunde D, Brinkmann R, Huber R, Tronnier V and Bonsanto MM (2023) The neurosurgical benefit of contactless in vivo optical coherence tomography regarding residual tumor detection: A clinical study. Front. Oncol. 13:1151149. doi: 10.3389/fonc.2023.1151149

@article{RN5430,
   author = {Hutfilz, Alessa;Theisen-Kunde, Dirk;Bonsanto, Matteo Mario and Brinkmann, Ralf},
   title = {Pulsed thulium laser blood vessel haemostasis as an alternative to bipolar forceps during neurosurgical tumour resection},
   journal = {Lasers in Medical Science},
   volume = {38},
   number = {1},
   pages = {94},
   ISSN = {1435-604X},
   DOI = {10.1007/s10103-023-03747-9},
   url = {https://doi.org/10.1007/s10103-023-03747-9},
   year = {2023},
   type = {Journal Article}
}

@article{doi:10.1021/acs.jpcb.2c09115,
author = {Gottschall, Thomas and Meyer-Zedler, Tobias and Eibl, Matthias and Pfeiffer, Tom and Hakert, Hubertus and Schmitt, Michael and Huber, Robert and Tünnermann, Andreas and Limpert, Jens and Popp, Juergen},
title = {Ultrafast Spectral Tuning of a Fiber Laser for Time-Encoded Multiplex Coherent Raman Scattering Microscopy},
journal = {The Journal of Physical Chemistry B},
volume = {0},
number = {0},
pages = {null},
year = {0},
doi = {10.1021/acs.jpcb.2c09115},
    note ={PMID: 36917762},
URL = {https://doi.org/10.1021/acs.jpcb.2c09115},
eprint = { https://doi.org/10.1021/acs.jpcb.2c09115}
}

@article{RN5362,
   author = {Yamamoto, Manabu;Miura, Yoko;Hirayama, Kumiko;Kyo, Akika;Kohno, Takeya;Theisen-Kunde, Dirk;Brinkmann, Ralf and Honda, Shigeru},
   title = {Comparative Treatment Study on Macular Edema Secondary to Branch Retinal Vein Occlusion by Intravitreal Ranibizumab with and without Selective Retina Therapy},
   journal = {Life},
   volume = {13},
   number = {3},
   pages = {769},
   ISSN = {2075-1729},
   DOI = {10.3390/life13030769},
   url = {https://www.mdpi.com/2075-1729/13/3/769},
   year = {2023},
   type = {Journal Article}
}

@article{STRAUCH2023e210,
title = {Accelerating intraoperative tumor histology with sectioning-free multiphoton microscopy},
journal = {European Journal of Surgical Oncology},
volume = {49},
number = {2},
pages = {e210},
year = {2023},
issn = {0748-7983},
doi = {https://doi.org/10.1016/j.ejso.2022.11.575},
url = {https://www.sciencedirect.com/science/article/pii/S0748798322013245},
author = {Matthias Strauch and Jan Philip Kolb and Christian Rose and Nadine Merg and Jennifer Hundt and Christiane Kümpers and Sven Perner and Sebastian Karpf and Robert Huber}
}

@article{Puyo:23,
author = {L\'{e}o Puyo and Clara Pf\"{a}ffle and Hendrik Spahr and Jonas Franke and Daniel Bublitz and Dierck Hillmann and Gereon H\"{u}ttmann},
journal = {Opt. Express},
keywords = {Fourier optics; Full field optical coherence tomography; Image quality; Imaging techniques; Spatial frequency; Spatial light modulators},
number = {20},
pages = {33500--33517},
publisher = {Optica Publishing Group},
title = {Diffuse-illumination holographic optical coherence tomography},
volume = {31},
month = {Sep},
year = {2023},
url = {https://opg.optica.org/oe/abstract.cfm?URI=oe-31-20-33500},
doi = {10.1364/OE.498654},
abstract = {Holographic optical coherence tomography (OCT) is a powerful imaging technique, but its ability to reveal low-reflectivity features is limited. In this study, we performed holographic OCT by incoherently averaging volumes with changing diffuse illumination of numerical aperture (NA) equal to the detection NA. While the reduction of speckle from singly scattered light is only modest, we discovered that speckle from multiply scattered light can be arbitrarily reduced, resulting in substantial improvements in image quality. This technique also offers the advantage of suppressing noises arising from spatial coherence, and can be implemented with a partially spatially incoherent light source for further mitigation of multiple scattering. Finally, we show that although holographic reconstruction capabilities are increasingly lost with decreasing spatial coherence, they can be retained over an axial range sufficient to standard OCT applications.},
}

@article{FU2023106664,
title = {Laser induced spherical bubble dynamics in partially confined geometry with acoustic feedback from container walls},
journal = {Ultrasonics Sonochemistry},
volume = {101},
pages = {106664},
year = {2023},
issn = {1350-4177},
doi = {https://doi.org/10.1016/j.ultsonch.2023.106664},
url = {https://www.sciencedirect.com/science/article/pii/S1350417723003760},
author = {Lei Fu and Xiao-Xuan Liang and Sijia Wang and Siqi Wang and Ping Wang and Zhenxi Zhang and Jing Wang and Alfred Vogel and Cuiping Yao},
keywords = {Laser-induced cavitation, Partial confinement, Acoustic feedback, Elastic wall, Vibrations, Extended Rayleigh-Plesset model},
abstract = {We investigated laser-induced cavitation dynamics in a small container with elastic thin walls and free or partially confined surface both experimentally and by numerical investigations. The cuvette was only 8–25 times larger than the bubble in its center. The liquid surface was either free, or two thirds were confined by a piston-shaped pressure transducer. Different degrees of confinement were realized by filling the liquid up to the transducer surface or to the top of the cuvette. For reference, some experiments were performed in free liquid. We recorded the bubble dynamics simultaneously by high-speed photography, acoustic measurements, and detection of probe beam scattering. Simultaneous single-shot recording of radius-time curves and oscillation times enabled to perform detailed investigations of the bubble dynamics as a function of bubble size, acoustic feedback from the elastic walls, and degree of surface confinement. The bubble dynamics was numerically simulated using a Rayleigh-Plesset model extended by terms describing the acoustically mediated feedback from the bubble’s environment. Bubble oscillations were approximately spherical as long as no secondary cavitation by tensile stress occurred. Bubble expansion was always similar to the dynamics in free liquid, and the environment influenced mainly the collapse phase and subsequent oscillations. For large bubbles, strong confinement led to a slight reduction of maximum bubble size and to a pronounced reduction of the oscillation time, and both effects increased with bubble size. The joint action of breakdown-induced shock wave and bubble expansion excites cuvette wall vibrations, which produce alternating pressure waves that are focused onto the bubble. This results in a prolongation of the collapse phase and an enlargement of the second oscillation, or in time-delayed re-oscillations. The details of the bubble dynamics depend in a complex manner on the degree of surface confinement and on bubble size. Numerical simulations of the first bubble oscillation agreed well with experimental data. They suggest that the alternating rarefaction/compression waves from breakdown-induced wall vibrations cause a prolongation of the first oscillation. By contrast, liquid mass movement in the cuvette corners result in wall vibrations causing late re-oscillations. The strong and rich interaction between the bubble and its surroundings may be relevant for a variety of applications such as intraluminal laser surgery and laser-induced cavitation in microfluidics.}
}

@article{Meyer:23,
author = {Stefan Meyer and Tonio F. Kutscher and Philipp Lamminger and Florian Sommer and Sebastian Karpf},
journal = {Opt. Continuum},
keywords = {Femtosecond pulses; Fluorescence lifetime imaging; Phase modulation; Picosecond pulses; Single mode lasers; Ultrashort pulses},
number = {11},
pages = {2298--2307},
publisher = {Optica Publishing Group},
title = {Leveraging the periodic interference condition in electro-optic modulators for picosecond pulse generation},
volume = {2},
month = {Nov},
year = {2023},
url = {https://opg.optica.org/optcon/abstract.cfm?URI=optcon-2-11-2298},
doi = {10.1364/OPTCON.500969},
abstract = {Ultra-short optical pulses in the femtosecond and picosecond regime are typically generated using mode-locked lasers. However, in mode-locking, the pulse repetition rate is fundamentally linked to the cavity length of the laser, making it difficult to synchronize these laser pulses to other light sources. Here, we apply a pulse-on-demand approach to picosecond pulse generation with an electro-optic intensity modulator (EOM). The high, 40 GHz bandwidth of the EOM enables low picosecond pulses, however it shifts the problem of pulse generation to the electronic pulses, requiring high bandwidth electronics. In this study, we present an electro-optic operation, leveraging the periodic interference condition of intensity EOMs by operating it with rising edges at twice its V$\pi$ voltage. Utilizing this method, pulse durations as short as 10.9 ps were achieved by employing a 35 ps edge from an arbitrary waveform generator. The pulses were measured directly on a high-speed oscilloscope as well as indirectly through the spectral broadening of the generated optical pulses. We employ this approach to show arbitrary pulse length generation by applying step functions with only one V$\pi$ voltage, thus permitting direct pulse-on-demand generation of pulses with arbitrary pulse length, shape and repetition rate for applications in spectroscopy, sensing and nonlinear imaging.},
}

@article{Kutscher:23,
author = {Tonio F. Kutscher and Philipp Lamminger and Anton Gruber and Christina Leonhardt and Annika Hunold and Robert A. Huber and Sebastian Karpf},
journal = {Opt. Lett.},
keywords = {Erbium-doped fiber amplifiers; Laser imaging; Laser sources; Lidar; Multiphoton microscopy; Picosecond pulses},
number = {23},
pages = {6096--6099},
publisher = {Optica Publishing Group},
title = {Pulsed swept-source FDML-MOPA laser with kilowatt picosecond pulses around 1550 nm},
volume = {48},
month = {Dec},
year = {2023},
url = {https://opg.optica.org/ol/abstract.cfm?URI=ol-48-23-6096},
doi = {10.1364/OL.500943},
abstract = {Swept-source lasers are versatile light sources for spectroscopy, imaging, and microscopy. Swept-source-powered multiphoton microscopy can achieve high-speed, inertia-free point scanning with MHz line-scan rates. The recently introduced spectro-temporal laser imaging by diffractive excitation (SLIDE) technique employs swept-source lasers to achieve kilohertz imaging rates by using a swept-source laser in combination with a diffraction grating for point scanning. Multiphoton microscopy at a longer wavelength, especially in the shortwave infrared (SWIR) region, can have advantages in deep tissue penetration or applications in light detection and ranging (LiDAR). Here we present a swept-source laser around 1550 nm providing high-speed wavelength agility and high peak power pulses for nonlinear excitation. The swept-source laser is a Fourier-domain mode-locked (FDML) laser operating at 326 kHz sweep rate. For high peak powers, the continuous wave (cw) output is pulse modulated to short picosecond pulses and amplified using erbium-doped fiber amplifiers (EDFAs) to peak powers of several kilowatts. This FDML-master oscillator power amplifier (FDML-MOPA) setup uses reliable, low-cost fiber components. As proof-of-principle measurement, we show third-harmonic generation (THG) using harmonic nanoparticles at the 10 MHz pulse excitation rate. This new, to the best of our knowledge, laser source provides unique performance parameters for applications in nonlinear microscopy, spectroscopy, and ranging.},
}

@article{RN5426,
   author = {Aşırım, Özüm Emre;Huber, Robert and Jirauschek, Christian},
   title = {Influence of the linewidth enhancement factor on the signal pattern of Fourier domain mode-locked lasers},
   journal = {Applied Physics B},
   volume = {128},
   number = {12},
   pages = {218},
   ISSN = {1432-0649},
   DOI = {10.1007/s00340-022-07933-5},
   url = {https://doi.org/10.1007/s00340-022-07933-5},
   year = {2022},
   type = {Journal Article}
}

@article{JACOBI2022S288,
title = {620 Screening an inhibitor library for new drug candidates to promote wound healing},
journal = {Journal of Investigative Dermatology},
volume = {142},
number = {12, Supplement },
pages = {S288},
year = {2022},
note = {ESDR 2022 Meeting Abstract Supplement},
issn = {0022-202X},
doi = {https://doi.org/10.1016/j.jid.2022.09.637},
url = {https://www.sciencedirect.com/science/article/pii/S0022202X22025714},
author = {C. Jacobi and M. Göb and R. Huber and R.J. Ludwig and J.E. Hundt}
}

@article{RN5359,
   author = {Ha-Wissel, L.;Yasak, H.;Huber, R.;Zillikens, D.;Ludwig, R. J.;Thaçi, D. and Hundt, J. E.},
   title = {Case report: Optical coherence tomography for monitoring biologic therapy in psoriasis and atopic dermatitis},
   journal = {Front Med (Lausanne)},
   volume = {9},
   pages = {995883},
   ISSN = {2296-858X (Print)
2296-858x},
   DOI = {10.3389/fmed.2022.995883},
   year = {2022},
   type = {Journal Article}
}

@article{Strenge-2022,
   author = {Strenge, P.;Lange, B.;Grill,C.;Danicke,V.;Theisen-Kunde, D.;Hagel, C.;Spahr-Hess, S.;;Bonsanto, Matteo M.;Handels, H.; and Huber, R.;Brinkmann, R.},
   title = {Differentiation of different stages of brain tumor infiltration using optical coherence tomography: Comparison of two systems and histology},
   journal = {Frontiers in Oncology},
Keywords = {AG-Huber_FDML, AG-Huber_OCT, brain, tumor, glioblastoma multiforme, OCT, neural network, attenuation (absorption)
coefficient, optical coherence tomography},
   DOI = {https://doi.org/10.3389/fonc.2022.896060},
   url = {https://www.frontiersin.org/articles/10.3389/fonc.2022.896060/full},
   year = {2022},
   type = {Journal Article}
}

@article{Grill2022,
  doi = {10.1038/s42005-022-00960-w},
  year = {2022},
  publisher = {Springer Science and Business Media {LLC}},
  volume = {{5}},
  number = {{1}},
  author = {C. Grill, T. Bl\"{o}mker, M. Schmidt, D. Kastner, T. Pfeiffer, J.P. Kolb, W. Draxinger, S. Karpf, C. Jirauschek and R. Huber},
  title = {Towards phase-stabilized Fourier domain mode-locked frequency combs},
  journal = {{Communications Physics}},
keywords={AG-Huber_FDML, FDML, Fourier domain mode locking, phase, frequency comb, coherence, beating}
}

@article{Strenge2022,
   author = {Strenge, P;Lange, B;Grill, C;Draxinger, W;Danicke, V;Theisen-Kunde, D;Hagel, C;Spahr-Hess, S;Bonsanto, Matteo M.;Huber, R;Handels, H and Brinkmann, R},
   title = {Registration of histological brain images onto optical coherence tomography images based on shape information},
keywords = {brain, glioblastoma multiforme, shape, OCT, optical coherence tomography, AG-Huber_OCT,},
   journal = {Physics in Medicine & Biology},
   ISSN = {0031-9155},
   url = {http://iopscience.iop.org/article/10.1088/1361-6560/ac6d9d},
   year = {2022},
   type = {Journal Article}
}

@article{RN5328,
   author = {Pfäffle, C;Spahr, H;Gercke, K;Puyo, L;Höhl, S;Melenberg, D;Miura, Y;Hüttmann, G and Hillmann, D},
   title = {Phase-Sensitive Measurements of Depth-Dependent Signal Transduction in the Inner Plexiform Layer},
   journal = {Frontiers in Medicine},
   volume = {9},
   ISSN = {2296-858X},
   DOI = {10.3389/fmed.2022.885187},
keywords = {optoretinography, optical coherence tomography, phase-sensitive OCT, functional imaging, inner
plexiform layer, retina},
   url = {https://www.frontiersin.org/articles/10.3389/fmed.2022.885187},
   year = {2022},
   type = {Journal Article}
}

@article{Deen2022,
   author = {Deen, A D;Van Beusekom, H M. M.;Pfeiffer, T;Stam, M;Kleijn, D De;Wentzel, J;Huber, R;Van Der Steen, A F. W.;Soest, G Van and Wang, T},
   title = {Spectroscopic thermo-elastic optical coherence tomography for tissue characterization},
   journal = {BioOptExpr},
keywords = {AG-Huber, Endoscopic imaging, Image processing, Image quality, Imaging techniques, Optical imaging, Tissue characterization},
   volume = {13(3)},
   pages = {1430-1446},
   DOI = {10.1364/BOE.447911},
    year = {2022},
   type = {Journal Article}
}

@article{Gob:22,
author = {Madita G\"{o}b and Tom Pfeiffer and Wolfgang Draxinger and Simon Lotz and Jan Philip Kolb and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {High speed imaging; Image processing; Image quality; In vivo imaging; Range imaging; Vertical cavity surface emitting lasers},
number = {2},
pages = {713--727},
publisher = {Optica Publishing Group},
title = {Continuous spectral zooming for in vivo live 4D-OCT with MHz A-scan rates and long coherence},
volume = {13},
month = {Feb},
year = {2022},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-13-2-713},
doi = {10.1364/BOE.448353},
abstract = {We present continuous three-dimensional spectral zooming in live 4D-OCT using a home-built FDML based OCT system with 3.28 MHz A-scan rate. Improved coherence characteristics of the FDML laser allow for imaging ranges up to 10 cm. For the axial spectral zoom feature, we switch between high resolution and long imaging range by adjusting the sweep range of our laser. We present a new imaging setup allowing for synchronized adjustments of the imaging range and lateral field of view during live OCT imaging. For this, a novel inline recalibration algorithm was implemented that enables numerical k-linearization of the raw OCT fringes for every frame instead of every volume. This is realized by acquiring recalibration data within the dead time of the raster scan at the turning points of the fast axis scanner. We demonstrate in vivo OCT images of fingers and hands at different resolution modes and show real three-dimensional zooming during live 4D-OCT. A three-dimensional spectral zooming feature for live 4D-OCT is expected to be a useful tool for a wide range of biomedical, scientific and research applications, especially in OCT guided surgery.},
}

@article{Yashin-2022,
   author = {Yashin, K;Bonsanto, M M;Achkasova, K;Zolotova, A;Wael, Al-M;Kiseleva, E;Moiseev, A;Medyanik, I;Kravets, L;Huber, R;Brinkmann, R and Gladkova, N},
   title = {OCT-Guided Surgery for Gliomas: Current Concept and Future Perspectives},
   journal = {Diagnostics},
   volume = {12},
   number = {2},
   pages = {335},
   ISSN = {2075-4418},
keywords = {AG-Huber; optical coherence tomography; brain imaging; neurosurgical guidance; brain tumor; minimally invasive theranostics; intraoperative imaging},
   url = {https://www.mdpi.com/2075-4418/12/2/335},
   year = {2022},
   type = {Journal Article}
}

@article{Liang2022,
   author = {Liang, X-X;Linz, N;Freidank, S;Paltauf, G and Vogel, A},
   title = {Comprehensive analysis of spherical bubble oscillations and shock wave emission in laser-induced cavitation},
keywords = {bubble dynamics, cavitation, shock waves},
   journal = {Journal of Fluid Mechanics},
   volume = {940},
   pages = {A5},
   ISSN = {0022-1120},
   DOI = {10.1017/jfm.2022.202},
  
   year = {2022},
   type = {Journal Article}
}

@article{Lange2022,
   author = {Lange, B;Ozimek, T;Wießmeyer, J R;Kramer, M W.;Merseburger, A S. and Brinkmann, R},
   title = {Theoretical and experimental evaluation of the distance dependence of fiber-based fluorescence and reflection measurements for laser lithotripsy},
   journal = {Biomedical Physics & Engineering Express},
   volume = {8},
   number = {5},
abstract = {Objectives. In laser lithotripsy, a green aiming beam overlying the infrared (IR) treatment radiation gives rise to reflection and fluorescence signals that can be measured via the treatment fiber. While stone autofluorescence is used for target detection, the condition of the fiber can be assessed based on its Fresnel reflection. For good applicability, fluorescence detection of stones should work even when the stone and fiber are not in direct contact. Fiber breakage detection, on the other hand, can be falsified if surfaces located in front of the fiber reflect light from the aiming laser back into it. For both applications, therefore, a fundamental investigation of the dependence of the signal amplitude on the distance between fiber and surface is important. Methods. Calculations of the signal drop of fluorescence or diffuse and specular reflection with increasing fiber distance were performed using ray tracing based on a simple geometric model for different fiber core diameters. Reflection signals from a mirror, diffuse reflector, human calculi, and porcine renal tissue placed in water were measured at varying distances (0–5 mm). For human calculi, fluorescence signals were recorded simultaneously. Results. The calculations showed a linear signal decrease down to ∼60% of the maximum signal (fiber in contact). The distance z at which the signal drops to for example 50% depends linearly on the diameter of the fiber core. For fibers used in lithotripsy and positioned in water, z50% ranges from 0.55 mm (200 μm core diameter) to 2.73 mm, (1 mm core diameter). The calculations were in good agreement with the experimental results. Conclusions. The autofluorescence signals of stones can be measured in non-contact mode. Evaluating the Fresnel signal of the end face of the fiber to detect breakage is possible unless the fiber is situated less than some millimeters to reflecting surfaces.},
keywords = {urolithiasis, laser lithotripsy, fluorescence, reflectance},
   pages = {055023},
   ISSN = {2057-1976},
   DOI = {10.1088/2057-1976/ac82c7},
   
   year = {2022},
   type = {Journal Article}
}

@article{Miura2022,
   author = {Miura, Y;Inagaki, K;Hutfilz, A;Seifert, E;Schmarbeck, B;Murakami, A;Ohkoshi, K and Brinkmann, R},
   title = {Temperature Increase and Damage Extent at Retinal Pigment Epithelium Compared between Continuous Wave and Micropulse Laser Application},
   journal = {Life},
   volume = {12(9)},
  
   pages = {1313},
   ISSN = {2075-1729},
   url = {https://www.mdpi.com/2075-1729/12/9/1313},
   year = {2022},
   type = {Journal Article}
}

@article{Liang2022,
   author = {Liang, X-X and Vogel, A},
   title = {Probing neuronal functions with precise and targeted laser ablation in the living cortex: comment},
   journal = {Optica},
   volume = {9(8)},
   keywords = {Attenuation coefficient, Femtosecond lasers, Laser ablation, Laser irradiation, Numerical simulation, Thermal effects},
   pages = {868-871},
   DOI = {10.1364/OPTICA.454469},  
   year = {2022},
   type = {Journal Article}
}

@article{von-der-Burchardt2022,
   author = {von der Burchard, C;Sudkamp, H;Tode, J;Ehlken, C;Purtskhvanidze, K;Moltmann, M;Heimes, B;Koch, P;Münst, M;vom Endt, M;Kepp, T;Theisen-Kunde, D;König, I;Hüttmann, G and Roider, J},
   title = {Self-Examination Low-Cost Full-Field Optical Coherence Tomography (SELFF-OCT) for neovascular age-related macular degeneration: a cross-sectional diagnostic accuracy study},
   journal = {BMJ Open},
   volume = {12},
   number = {6},
   pages = {e055082},
   DOI = {10.1136/bmjopen-2021-055082},
   url = {http://bmjopen.bmj.com/content/12/6/e055082.abstract},
   year = {2022},
   type = {Journal Article}
}

@article{Schaller2022,
   author = {Schaller, M;Wilson, M;Kleyman, V;Mordmüller, M;Brinkmann, R;Müller, M. A. and Worthmann, K},
   title = {Parameter estimation and model reduction for model predictive control in retinal laser treatment},
   journal = {Control Engineering Practice},
   volume = {128},
   pages = {105320},
   ISSN = {0967-0661},
   DOI = {https://doi.org/10.1016/j.conengprac.2022.105320},

   year = {2022},
   type = {Journal Article}
}

@article{Schaller2022,
   author = {Schaller, M.;Kleyman, K.;Mordmüller, M.;Schmidt, C.;Wilson, M.;Brinkmann, R.;Müller, M.A. and Worthmann, K.},
   title = {Model predictive control for retinal laser treatment at 1 kHz},
   journal = {at - Automatisierungstechnik},
   volume = {70(11)},
   keywords = {model predictive control; real-time control;retinal photocoagulation},
   pages = {992-1002},
  
   url = {https://doi.org/10.1515/auto-2022-0030},
   year = {2022},
   type = {Journal Article}
}

@article{GOODWIN2022,
title = {Detection of subtle cartilage and bone tissue degeneration in the equine joint using polarisation-sensitive optical coherence tomography},
journal = {Osteoarthritis and Cartilage},
year = {2022},
issn = {1063-4584},
doi = {https://doi.org/10.1016/j.joca.2022.04.006},
author = {M. Goodwin, M. Klufts, J. Workman, A. Thambyah and F. Vanholsbeeck},
keywords = {Optical coherence tomography, Polarisation-sensitive optical coherence tomography, Osteoarthritis},
abstract = {Summary
Objective
To explore the ability of polarisation-sensitive optical coherence tomography (PS-OCT) to rapidly identify subtle signs of tissue degeneration in the equine joint.
Method
Polarisation-sensitive optical coherence tomography (PS-OCT) images were systematically acquired in four locations along the medial and lateral condyles of the third metacarpal bone in five dissected equine specimens. Intensity and retardation PS-OCT images, and anomalies observed therein, were then compared and validated with high resolution images of the tissue sections obtained using Differential Interference contrast (DIC) optical light microscopy.
Results
The PS-OCT system was capable of imaging the entire equine osteochondral unit, and allowed delineation of the three structurally differentiated zones of the joint, that is, the articular cartilage matrix, zone of calcified cartilage and underlying subchondral bone. Importantly, PS-OCT imaging was able to detect underlying matrix and bone changes not visible without dissection and/or microscopy.
Conclusion
PS-OCT has substantial potential to detect, non-invasively, sub-surface microstructural changes that are known to be associated with the early stages of joint tissue degeneration.}
}

@article{Freidank2022,
   author = {Freidank, S;Vogel, A and Linz, N},
   title = {Mechanisms of corneal intrastromal laser dissection for refractive surgery: ultra-high-speed photographic investigation at up to 50 million frames per second},
   journal = {BioOptExpr},
   volume = {13 (5)},
   
   pages = {3056-3079},
   DOI = {10.1364/BOE.455926},

   year = {2022},
   type = {Journal Article}
}

Optical coherence elastography (OCE), a functional extension of optical coherence tomography (OCT), visualizes tissue strain to deduce the tissue’s biomechanical properties. In this study, we demonstrate intravascular OCE using a 1.1 mm motorized catheter and a 1.6 MHz Fourier domain mode-locked OCT system. We induced an intraluminal pressure change by varying the infusion rate from the proximal end of the catheter. We analysed the pixel-matched phase change between two different frames to yield the radial strain. Imaging experiments were carried out in a phantom and in human coronary arteries in vitro. At an imaging speed of 3019 frames/s, we were able to capture the dynamic strain. Stiff inclusions in the phantom and calcification in atherosclerotic plaques are associated with low strain values and can be distinguished from the surrounding soft material, which exhibits elevated strain. For the first time, circumferential intravascular OCE images are provided side by side with conventional OCT images, simultaneously mapping both the tissue structure and stiffness.

@article{Kohlfarber2022,
   author = {Kohlfaerber, T;Pieper, M;Münter, M;Holzhausen, C;Ahrens, M;Idel, C;Bruchhage, K-L;Leichtle, A;König, P;Hüttmann, G and Schulz-Hildebrandt, H},
   title = {Dynamic microscopic optical coherence tomography to visualize the morphological and functional micro-anatomy of the airways},
   journal = {Biomedical Optics Express},
   volume = {13 (6)},
   pages = {3211-3223},
   DOI = {10.1364/BOE.456104},
  
   year = {2022},
   type = {Journal Article}
}

@article{Musial-2022,
   author = {Musial, G;Kohlfaerber, T;Ahrens, M;Schulz-Hildebrandt, H;Steven, P and Hüttmann, G},
   title = {Dynamic Contrast Microscopic Optical Coherence Tomography As a Novel Method for Assessing Corneal Epithelium During Exposure to Benzalkonium Chloride},
   journal = {Translational Vision Science & Technology},
keywords = {toxicity; optical coherence tomography; benzalkonium chloride},
   volume = {11(5)},

   pages = {28-28},
   ISSN = {2164-2591},
   DOI = {10.1167/tvst.11.5.28},
   url = {https://doi.org/10.1167/tvst.11.5.28},
   year = {2022},
   type = {Journal Article}
}

@article{Hakert2021,
   author = {H. Hakert, M. Eibl, M. Tillich, R.Pries, G. Hüttmann, R. Brinkmann, B. Wollenberg, K-L. Bruchhage, S. Karpf and R. Huber},
   title = {Time-encoded stimulated Raman scattering microscopy of tumorous human pharynx tissue in the fingerprint region from 1500–1800  cm-1},
   journal = {Optics Letters},
   volume = {46(14)},
   number = {14},
   pages = {3456-3459},
keywords = {AG-Huber_NL, Clinical applications, Master oscillator power amplifiers, Optical coherence tomography, Raman scattering, Stimulated Raman scattering, Stimulated scattering},
   DOI = {https://doi.org/10.1364/OL.424726},
   year = {2021},
   type = {Journal Article}
}

@article{PenateMedina2021,
   author = {Peñate Medina, T;Kolb, J P;Hüttmann, G;Huber, R;Peñate Medina, O;Ha, L;Ulloa, P;Larsen, N;Ferrari, A;Rafecas, M;Ellrichmann, M;Pravdivtseva, M S.;Anikeeva, M;Humbert, J;Both, M;Hundt, J E. and Hövener, J-B},
   title = {Imaging Inflammation - From Whole Body Imaging to Cellular Resolution},
   journal = {Frontiers in immunology},
keywords = {AG-Huber, MRI, PET, SPECT, optical imaging, Optical coherence tomography (OCT), precision medicine, Two-Photon microscopy (TPM), hyperpolarization},
   volume = {12},
   pages = {692222-692222},
   ISSN = {1664-3224},
   DOI = {10.3389/fimmu.2021.692222},
   url = {https://pubmed.ncbi.nlm.nih.gov/34248987
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264453/},
   year = {2021},
   type = {Journal Article}
}

@article{Gottschall2021,
   author = {T. Gottschall, T. Meyer-Zedler, M. Schmitt, R. Huber, J. Popp, A. Tünnermann and J. Limpert},
   title = {Ultra-compact tunable fiber laser for coherent anti-Stokes Raman imaging},
   journal = {JRS},
  keywords = { AG-Huber_NL, coherent anti-Stokes Raman scattering microscopy, four-wave mixing, nonlinear
microscopy, ultrafast laser},
   ISSN = {0377-0486},   
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/jrs.6171},
   year = {2021},
   type = {Journal Article}
}

@article{Lotz2021,
   author = {S. Lotz, C. Grill, M. Göb, W. Draxinger, J.P. Kolb and R. Huber},
   title = {Cavity length control for Fourier domain mode locked (FDML) lasers with µm precision},
   journal = {Biomedical Optics Express},
   volume = {12(5)},
   keywords={AG-Huber_FDML},
   pages = {2604-2616},
   url = {https://doi.org/10.1364/BOE.422898},
   year = {2021},
   type = {Journal Article}
}

@Article{Schmidt2021,
  author   = {M. Schmidt, C. Grill, S. Lotz, T. Pfeiffer, R. Hubert and C. Jirauschek},
  journal  = {Applied Physics B},
  title    = {Intensity pattern types in broadband Fourier domain mode-locked (FDML) lasers operating beyond the ultra-stable regime},
  year     = {2021},
  issn     = {1432-0649},
  number   = {5},
  pages    = {60},
  volume   = {127},
keywords={AG-Huber_FDML},
  abstract = {We report on the formation of various intensity pattern types in detuned Fourier domain mode-locked (FDML) lasers and identify the corresponding operating conditions. Such patterns are a result of the complex laser dynamics and serve as an ideal tool for the study of the underlying physical processes as well as for model verification. By numerical simulation we deduce that the formation of patterns is related to the spectral position of the instantaneous laser lineshape with respect to the transmission window of the swept bandpass filter. The spectral properties of the lineshape are determined by a long-term accumulation of phase-offsets, resulting in rapid high-amplitude intensity fluctuations in the time domain due to the narrow intra-cavity bandpass filter and the fast response time of the semiconductor optical amplifier gain medium. Furthermore, we present the distribution of the duration of dips in the intensity trace by running the laser in the regime in which dominantly dips form, and give insight into their evolution over a large number of roundtrips.},
  doi      = {10.1007/s00340-021-07600-1},
  refid    = {Schmidt2021},
}

@article{Mordmüller-2021,
   author = {Mordmüller, M;Kleyman, V;Schaller, M;Wilson, M;Theisen-Kunde, D;Worthmann, K;Müller, M.A and Brinkmann, R},
   title = {Towards temperature controlled retinal laser treatment with a single laser at 10 kHz repetition rate},
   journal = {Advanced Optical Technologies},
Keywords = {extended Kalman filter; laser-coagulation; model predictive control; ophthalmology; photo-acoustics},
  
   url = {https://doi.org/10.1515/aot-2021-0041},
   year = {2021},
   type = {Journal Article}
}

@article{Coker2021,
   author = {Coker, Z. N.;Liang, Xiao-Xuan;Kiester, A. S.;Noojin, G. D.;Bixler, J. N.;Ibey, B. L.;Vogel, A. and Yakovlev, V. V.},
   title = {Synergistic effect of picosecond optical and nanosecond electrical pulses on dielectric breakdown in aqueous solutions},
   journal = {Photonics Research},
   volume = {9 (3)},
   pages = {416-423},
   DOI = {10.1364/PRJ.411980},
 
   year = {2021},
   type = {Journal Article}
}

@article{Kleyman2021,
   author = {Kleyman, V;Schaller, M;Wilson, M;Mordmüller, M;Brinkmann, R;Worthmann, K and Müller, M.A.},
   title = {State and parameter estimation for model-based retinal laser treatment⁎⁎The collaborative project ”Temperature controlled retinal laser treatment” is funded by the German Research Foundation (DFG) under the project number 430154635 (MU 3929/3-1, WO 2056/7-1, BR 1349/6-1). MS was also funded by the DFG (grant WO 2056/2-1, project number 289034702). KW gratefully acknowledges funding by the German Research Foundation (DFG; grant WO 2056/6-1, project number 406141926)},
   journal = {IFAC-PapersOnLine},
   volume = {54(6)},
 
   pages = {244-250},
   ISSN = {2405-8963},
   DOI = {https://doi.org/10.1016/j.ifacol.2021.08.552},
   url = {https://www.sciencedirect.com/science/article/pii/S2405896321013276},
   year = {2021},
   type = {Journal Article}
}

@article{Richert2021,
   title        = {Selective retina therapy and thermal stimulation of the retina: different regenerative properties - implications for AMD therapy},
   author       = {Richert, E;Papenkort, J;von der Burchard, C;Klettner, A;Arnold, P;Lucius, R;Brinkmann, R;Framme, C;Roider, J and Tode, J},
   year         = 2021,
   journal      = {BMC Ophthalmology},
   volume       = {21(1)},
   pages        = 412,
   issn         = {1471-2415},
   url          = {https://doi.org/10.1186/s12886-021-02188-8},
   keywords     = {Selective retina therapy (SRT), Thermal stimulation of the retina (TSR),  Age- related macular  degeneration (AMD), Regeneration, Rejuvenation},
   type         = {Journal Article}
}

@article{Büttner2021,
   author = {Büttner, M.;Luger, B.;Abou Moulig, W.;Junker, B.;Framme, C.;Jacobsen, C.;Knoll, K. and Pielen, A.; SRT Study Group(Brinkmann, R.; Miura, Y.)},
   title = {Selective retina therapy (SRT) in patients with therapy refractory persistent acute central serous chorioretinopathy (CSC): 3 months functional and morphological results},
   journal = {Graefes Arch Clin Exp Ophthalmol},
   volume = {259},
   number = {6},
   pages = {1401-1410},
   ISSN = {0721-832X (Print)
0721-832x},
   DOI = {10.1007/s00417-020-04999-9},
abstract = { PURPOSE: Central serous chorioretinopathy (CSC) is a disease presenting with detachment of the neurosensory retina and characteristic focal leakage on fluorescein angiography. The spontaneous remission rate is 84% within 6 months. In this study, the efficacy of selective retina therapy (SRT) was examined in patients with therapy refractory persistent acute CSC defined by symptoms for at least 6 months and persistent subretinal fluid (SRF) despite eplerenone therapy. MATERIAL AND METHODS: This is a prospective, monocentric observational study in 17 eyes (16 patients, mean age 42 years, 2 female). SRT was performed with the approved R:GEN laser (Lutronic, South Korea), a micropulsed 527-nm Nd:YLF laser device, with a train of 30 pulses of 1.7 μs at 100-Hz repetition rate at the point of focal leakage determined by fluorescein angiography (FA) at baseline (BSL). Visits on BSL, week 4 (wk4), and week 12 (wk12) included best corrected visual acuity (BCVA, logMar), central retinal thickness (CRT) on spectral domain optical coherence tomography (SD-OCT), and FA. Statistical analysis was performed by pair-by-pair comparisons of multiple observations in each case with Bonferroni correction for multiple testing. (IBM SPSS Statistics 25®). RESULTS: Mean CRT at BSL was 387.69 ± 110.4 μm. CRT significantly decreased by 106.31 μm in wk4 (95%-KI: 21.42-191.2; p = 0.01), by 133.63 μm in wk12 (95%-KI: 50.22-217.03; p = 0.001) and by 133.81 μm (95%-KI: 48.88-218.75; p = 0.001) compared to BSL. Treatment success defined as complete resolution of SRF occurred at wk4 in 7/17 eyes (35.3%) and at wk12 in 10/17 eyes (58.8%). Re-SRT was performed in 7/17 eyes (41.2%) after an average of 107.14 ± 96.59 days. Treatment success after Re-SRT was observed in 4/6 eyes (66.6%, 12 weeks after Re-SRT). Mean BCVA did not change significantly from BSL to any later timepoint after adjusting for multiple testing. Notably, eyes with treatment success showed better BCVA at all timepoints and gained more letters compared to failures. CONCLUSION: Single or repetitive SRT may be an effective and safe treatment in 2 of 3 patients suffering from acute persistent CSC after 6 months of symptoms or more. We observed complete resolution of SRF in around 60% of eyes 12 weeks after first SRT treatment and also 12 weeks after Re-SRT treatment in eyes with persistent or recurrent SRF. Results on the long-term course after SRT are still pending.},
keywords = { Central serous chorioretinopathy; Fluorescein angiography; Micropulse laser; OCT; Persistent acute disease; Selective retina treatment; Subretinal fluid. },
   year = {2021},
   type = {Journal Article}
}

@article{Münter2021,
   author = {M. Münter, M. Pieper, T. Kohlfaerber, E. Bodenstorfer, M. Ahrens, C. Winter, R. Huber, P. König, G. Hüttmann and H. Schulz-Hildebrandt},
   title = {Microscopic optical coherence tomography (mOCT) at 600 kHz for 4D volumetric imaging and dynamic contrast},
   journal = {BiomedOptE},
   volume = {12(10)},
   Keywords = {CMOS cameras,Full field optical coherence tomography,High numerical aperture optics, Image processing,In vivo imaging,Medical imaging,Ag-Huber},
   pages = {6024-6039},
   DOI = {10.1364/BOE.425001},
  
   year = {2021},
   type = {Journal Article}
}

@article{Seifert2021,
   author = {Seifert, E;Sonntag, S R;Kleingarn, P;Theisen-Kunde, D;Grisanti, S;Birngruber, R;Miura, Y and Brinkmann, R},
   title = {Investigations on Retinal Pigment Epithelial Damage at Laser Irradiation in the Lower Microsecond Time Regime},
   journal = {Investigative Ophthalmology & Visual Science},
   volume = {62(3)},
  
   pages = {32-32},
   ISSN = {1552-5783},
   DOI = {10.1167/iovs.62.3.32},
   url = {https://doi.org/10.1167/iovs.62.3.32},
   year = {2021},
   type = {Journal Article}
}

@article{Miura-3,
   author = {Miura, Y},
   title = {Fluorescence Lifetime Imaging Ophthalmoscopy—FLIO},
   journal = {Nippon Laser Igakkaishi},
   volume = {advpub},
   DOI = {10.2530/jslsm.jslsm-42_0008},
   year = {2021},
   type = {Journal Article}
}

@article{Jackle2021,
abstract = {Background: In endovascular aneuysm repair (EVAR) procedures, medical instruments are currently navigated with a two-dimensional imaging based guidance requiring X-rays and contrast agent. Methods: Novel approaches for obtaining the three-dimensional instrument positions are introduced. Firstly, a method based on fibre optical shape sensing, one electromagnetic sensor and a preoperative computed tomography (CT) scan is described. Secondly, an approach based on image processing using one 2D fluoroscopic image and a preoperative CT scan is introduced. Results: For the tracking based method, average errors from 1.81 to 3.13 mm and maximum errors from 3.21 to 5.46 mm were measured. For the image-based approach, average errors from 3.07 to 6.02 mm and maximum errors from 8.05 to 15.75 mm were measured. Conclusion: The tracking based method is promising for usage in EVAR procedures. For the image-based approach are applications in smaller vessels more suitable, since its errors increase with the vessel diameter.},
author = {J{\"{a}}ckle, Sonja and Lange, Annkristin and Garcia-Vazquez, Veronica and Eixmann, Tim and Matysiak, Florian and Sieren, Malte Maria and Horn, Marco and Schulz-Hildebrandt, Hinnerk and H{\"{u}}ttmann, Gereon and Ernst, Floris and Heldmann, Stefan and P{\"{a}}tz, Torben and Preusser, Tobias},
doi = {10.1002/rcs.2327},
file = {:Users/schulz-hildebrandt/Documents/Mendeley Desktop/J{\"{a}}ckle et al/International Journal of Medical Robotics and Computer Assisted Surgery/J{\"{a}}ckle et al. - 2021 - Instrument localisation for endovascular aneurysm repair Comparison of two methods based on tracking systems or.pdf:pdf},
issn = {1478596X},
journal = {International Journal of Medical Robotics and Computer Assisted Surgery},
keywords = {2D/3D registration,3D localisation,computer-assisted surgery,electromagnetic tracking system,endovascular procedures,fibre optical shape sensing},
number = {6},
pages = {e2327},
year = {2021},
title = {{Instrument localisation for endovascular aneurysm repair: Comparison of two methods based on tracking systems or using imaging}},
volume = {17}
}

@article{Prasuhn2021,
   author = {Prasuhn, M;Miura, Y;Tura, A.;Rommel, Felix;Kakkassery, V;Sonntag, S;Grisanti, S and Ranjbar, M},
   title = {Influence of Retinal Microsecond Pulse Laser Treatment in Central Serous Chorioretinopathy: A Short-Term Optical Coherence Tomography Angiography Study},
   journal = {J ClinMed},
   volume = {10(11)},
   
   pages = {2418},
   ISSN = {2077-0383},
   url = {https://www.mdpi.com/2077-0383/10/11/2418},
   year = {2021},
   type = {Journal Article}
}

@article{Miura2021-2,
   author = {Sonntag, S R;Seifert, E;Hamann, M;Lewke, B;Theisen-Kunde, D;Grisanti, S;Brinkmann, R and Miura, Y},
   title = {Fluorescence Lifetime Changes Induced by Laser Irradiation: A Preclinical Study towards the Evaluation of Retinal Metabolic States},
   journal = {Life},
   volume = {11(6)},
  keywords = {retinal laser treatment; metabolic change; fluorescence lifetime imaging ophthalmoscopy},
   pages = {555},
   ISSN = {2075-1729},
   url = {https://www.mdpi.com/2075-1729/11/6/555},
   year = {2021},
   type = {Journal Article}
}

@article{Kyo-2021,
   author = {Kyo, A.;Yamamoto, M.;Hirayama, K.;Kohno, T.;Theisen-Kunde, D.;Brinkmann, R.;Miura, Y. and Honda, S.},
   title = {Factors affecting resolution of subretinal fluid after selective retina therapy for central serous chorioretinopathy},
   journal = {Sci Rep},
   volume = {11(1)},
  
   pages = {8973},
   ISSN = {2045-2322},
   DOI = {10.1038/s41598-021-88372-8},
   year = {2021},
   type = {Journal Article}
}

@article{Burri2021,
   author = {Burri, C;Hutfilz, A;Grimm, L;Salzmann, S;Arnold, P;Považay, B;Meier, C;Ebneter, A;Theisen-Kunde, D and Brinkmann, R},
   title = {Dynamic OCT Signal Loss for Determining RPE Radiant Exposure Damage Thresholds in Microsecond Laser Microsurgery},
   journal = {Applied Sciences},
   volume = {11(12)},
   
   pages = {5535},
   ISSN = {2076-3417},
   DOI = { https://doi.org/10.3390/app11125535},
   url = {https://www.mdpi.com/2076-3417/11/12/5535},
   year = {2021},
keywords = {selective retina therapy; viability assay; photocoagulation; microbubble formation;
thermomechanical damage; fringe washout; coherence-loss},
   type = {Journal Article}
}

@article{Cereda-2021,
   author = {Cereda, M G;Parrulli, S;Douven, Y. G. M.;Faridpooya, K;van Romunde, S;Hüttmann, G;Eixmann, T;Schulz-Hildebrandt, H;Kronreif, G;Beelen, M and de Smet, M D.},
   title = {Clinical Evaluation of an Instrument-Integrated OCT-Based Distance Sensor for Robotic Vitreoretinal Surgery},
   journal = {Ophthalmology Science},
   volume = {1(4)},
  
   pages = {100085},
   ISSN = {2666-9145},
   DOI = {https://doi.org/10.1016/j.xops.2021.100085},
  
   year = {2021},
   type = {Journal Article}
}

@article{Seifert2021,
   author = {Seifert, E;Tode, J;Pielen, A;Theisen-Kunde, D;Framme, C;Roider, J;Miura, Y;Birngruber, R and Brinkmann, R},
   title = {Algorithms for optoacoustically controlled selective retina therapy (SRT)},
   journal = {Photoacoustics},
Keywords = {SRT; Lasers in medicine; Ophthalmology; RPE; Selectivity; Algorithm; Retina therapy; Optoacoustics; Feedback},
   volume = {25},
   pages = {100316},
   ISSN = {2213-5979},
   url = {https://www.sciencedirect.com/science/article/pii/S2213597921000756},
   year = {2021},
   type = {Journal Article}
}

@article{Schenk-2021,
   author = {Schenk, M S;Wartak, A;Buehler, V;Zhao, J;Tearney, G J;Birngruber, R and Kassumeh, S},
   title = {Advances in Imaging of Subbasal Corneal Nerves With Micro–Optical Coherence Tomography},
   journal = {Tvst},
keywords = {corneal nerves; micro–optical coherence tomography; subbasal plexus},
   volume = {10 (13)},
   pages = {22-22},
   ISSN = {2164-2591},
   DOI = {10.1167/tvst.10.13.22},

   year = {2021},
   type = {Journal Article}
}

@article{Jaeckle2021c,
author = {J{\"{a}}ckle, Sonja and Eixmann, Tim and Matysiak, Florian and Sieren, Malte Maria and Horn, Marco and Schulz-Hildebrandt, Hinnerk and H{\"{u}}ttmann, Gereon and P{\"{a}}tz, Torben},
doi = {doi:10.1515/cdbme-2021-1004},
journal = {Current Directions in Biomedical Engineering},
year = {2021},
number = {1},
pages = {17--20},
title = {{3D Stent Graft Guidance based on Tracking Systems for Endovascular Aneurysm Repair:}},
url = {https://doi.org/10.1515/cdbme-2021-1004},
volume = {7},
}

@article{von-derBurchard2020,
   author = {von der Burchard, C;Moltmann, M.;Tode, J;Ehlken, C;Sudkamp, H;Theisen-Kunde, D;König, I;Hüttmann, G and Roider, J},
   title = {Self-examination low-cost full-field OCT (SELFF-OCT) for patients with various macular diseases},
   journal = {Graefe's Archive for Clinical and Experimental Ophthalmology},
   ISSN = {1435-702X},
   
   url = {https://doi.org/10.1007/s00417-020-05035-6},
   year = {2020},
   type = {Journal Article}
}

@article{Pfeiffer:20,
author = {T. Pfeiffer, M. G\"{o}b, W. Draxinger, S. Karpf, J.P. Kolb and R. Huber},
journal = {Biomed. Opt. Express},
keywords = {AG-Huber_OCT; High speed imaging; Image quality; Optical coherence tomography; Swept lasers; Swept sources; Systems design},
number = {11},
pages = {6799--6811},
publisher = {OSA},
title = {Flexible A-scan rate MHz-OCT: efficient computational downscaling by coherent averaging},
volume = {11},
month = {Nov},
year = {2020},
doi = {10.1364/BOE.402477},
abstract = {In order to realize adjustable A-scan rates of fast optical coherence tomography (OCT) systems, we investigate averaging of OCT image data acquired with a MHz-OCT system based on a Fourier Domain Mode Locked (FDML) laser. Increased system sensitivity and image quality can be achieved with the same system at the cost of lower imaging speed. Effectively, the A-scan rate can be reduced in software by a freely selectable factor. We demonstrate a detailed technical layout of the strategies necessary to achieve efficient coherent averaging. Since there are many new challenges specific to coherent averaging in swept source MHz-OCT, we analyze them point by point and describe the appropriate solutions. We prove that coherent averaging is possible at MHz OCT-speed without special interferometer designs or digital phase stabilization. We find, that in our system up to \&\#x223C;100x coherent averaging is possible while achieving a sensitivity increase close to the ideal values. This corresponds to a speed reduction from 3.3 MHz to 33 kHz and a sensitivity gain of 20 dB. We show an imaging comparison between coherent and magnitude averaging of a human finger knuckle joint in vivo with 121\&\#x00A0;dB sensitivity for the coherent case. Further, the benefits of computational downscaling in low sensitivity MHz-OCT systems are analyzed.},
}

@article{RN5351,
   author = {Richert, E;von der Burchard, C;Klettner, A;Arnold, P;Lucius, R;Brinkmann, R;Roider, J and Tode, J},
   title = {Modulation of inflammatory processes by thermal stimulating and RPE regenerative laser therapies in age related macular degeneration mouse models},
   journal = {Cytokine: X},
   volume = {2},
   number = {3},
   pages = {100031},
   ISSN = {2590-1532},
   DOI = {https://doi.org/10.1016/j.cytox.2020.100031},
   url = {https://www.sciencedirect.com/science/article/pii/S2590153220300112},
   year = {2020},
   type = {Journal Article}
}

@article{schmidt2020self,
  title={Self-stabilization mechanism in ultra-stable Fourier domain mode-locked (FDML) lasers},
  author={M. {Schmidt}, T. {Pfeiffer}, C. {Grill}, R. {Huber} and C. {Jirauschek}},
  journal={OSA Continuum},
  volume={3},
  number={6},
  pages={1589--1607},
  year={2020},
keywords={AG-Huber_FDML},
url={https://doi.org/10.1364/OSAC.389972}, 
  publisher=  {Optical Society of America}
}

@article{Cecchetti2020,
  doi = {10.1007/s10554-020-01796-7},
  url = {https://doi.org/10.1007/s10554-020-01796-7},
  year = {2020},
  month = feb,
  publisher = {Springer Science and Business Media {LLC}},
  volume = {36},
  number = {6},
  pages = {1021--1029},
  author = {Leonardo Cecchetti and Tianshi Wang and Ayla Hoogendoorn and Karen T. Witberg and Jurgen M. R. Ligthart and Joost Daemen and Heleen M. M. van Beusekom and Tom Pfeiffer and Robert A. Huber and Jolanda J. Wentzel and Antonius F. W. van der Steen and Gijs van Soest},
  title = {In-vitro and in-vivo imaging of coronary artery stents with Heartbeat {OCT}},
  journal = {The International Journal of Cardiovascular Imaging}
}

@article{Vogel-2020,
   author = {Kilin, V;Campargue, G;Fureraj, I;Sakong, S;Sabri, T;Riporto, F;Vieren, A;Mugnier, Y;Mas, C;Staedler, D;Collins, J M;Bonacina, L;Vogel, A;Capobianco, J A. and Wolf, J-P},
   title = {Wavelength-Selective Nonlinear Imaging and Photo-Induced Cell Damage by Dielectric Harmonic Nanoparticles},
   journal = {ACS Nano},
   volume = {14(4)},
   
   pages = {4087-4095},
   ISSN = {1936-0851},
   
   url = {https://doi.org/10.1021/acsnano.9b08813},
   year = {2020},
   type = {Journal Article}
}

@article{Birngruber2020,
   author = {Elhardt, C;Wertheimer, C M.;Wartak, A;Zhao, J;Leung, H M;Kassumeh, S A.;Yin, B;Tearney, G J. and Birngruber, R},
   title = {Stromal Nerve Imaging and Tracking Using Micro-Optical Coherence Tomography},
   journal = {Translational Vision Science & Technology},
   volume = {9(5)},
  
   pages = {6-6},
   ISSN = {2164-2591},
   Keywords = {optical coherence tomography; micro-OCT; imaging;corneal nerves; diabetes},
   url = {https://doi.org/10.1167/tvst.9.5.6},
   year = {2020},
   type = {Journal Article}
}

@article{Karpf-2020,
   author = {S. Karpf, C.T. Riche, D. Di Carlo, A. Goel, W.A. Zeiger, A. Suresh, C. Portera-Cailliau B. and Jalali},
   title = {Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates},
   journal = {Nature Communications},
   volume = {11(1)},
  keywords={},
   pages = {2062},
   ISSN = {2041-1723},
  
   url = {https://doi.org/10.1038/s41467-020-15618-w},
   year = {2020},
   type = {Journal Article}
}

@article{Wartak2020,
   author = {Wartak, A;Schenk, M S.;Bühler, V;Kassumeh, S A.;Birngruber, R and Tearney, G J.},
   title = {Micro-optical coherence tomography for high-resolution morphologic imaging of cellular and nerval corneal micro-structures},
keywords = {High numerical aperture optics,Image metrics,Image processing,Image quality,Optical coherence tomography,Optical imaging},
   journal = {Biomedical Optics Express},
   volume = {11(10)},
  
DOI = { https://doi.org/10.1364/BOE.402971},
   pages = {5920-5933},
   
   year = { 2020},
   type = {Journal Article}
}

@article{yamamoto2020,
   author = {Yamamoto, M;Miura, Y;Kyo, A;Hirayama, K;Kohno, T;Theisen-Kunde, D;Brinkmann, R and Honda, S},
   title = {Selective retina therapy for subretinal fluid associated with choroidal nevus},
   journal = {Amer J Ophthalm Case Rep},
   volume = {19},
   pages = {100794},
   ISSN = {2451-9936},
keywords = {Laser therapy, Choroidal tumor, Retinal pigment epithelium, Retinal disorder},
   DOI = {https://doi.org/10.1016/j.ajoc.2020.100794},
   
   year = {2020},
   type = {Journal Article}
}

@article{Hirayama2020,
   author = {Hirayama, K.;Yamamoto, M.;Kohno, T.;Kyo, A.;Theisen-Kunde, D.;Brinkmann, R.;Miura, Y. and Honda, S.},
   title = {Selective retina therapy (SRT) for macular serous retinal detachment associated with tilted disc syndrome},
   journal = {Graefes Arch Clin Exp Ophthalmol},
   ISSN = {0721-832x},
 volume = {259},
   pages = {387-393},
   DOI = {10.1007/s00417-020-04931-1},
   year = {2020},
   type = {Journal Article}
}

@article{Müller2020,
   author = {Müller M;Rahmanzadeh R;Tran T;Kappelhoff J;Akam EA;Caravan P;Jüstel T;Held KD;Anderson R and M, and Purschke},
   title = {Particle size of X-ray pumped UVC emitting nanoparticles defines intracellular localization and biological activity against cancer cells},
   journal = {Particle and Particle Systems Characterization },
   year = {2020},
   type = {Journal Article},
url = { https://onlinelibrary.wiley.com/toc/15214117/0/0}
}

@article{Richert2020,
   author = {Richert, E;Papenkort, J;Klettner, A;Tode, J;Koinzer, S;Brinkmann, R;Fink, C;Roeder, T;Lucius, R. and Roider, J},
   title = {Response of Retinal Pigment Epithelium (RPE)‐Choroid Explants to Thermal Stimulation Therapy of the RPE (TSR)},
   journal = {Lasers in Surgery and Medicine},
Keywords = {age‐related macular degeneration; thermal stimulation therapy of the retinal pigment epithelium;
matrix metalloproteases; pigment epithelium derived factor; retinal pigment epithelium; vascular endothelial
growth factor; transforming growth factor‐β},
   DOI = {DOI 10.1002/lsm.23288},
   year = {2020},
   type = {Journal Article}
}

@article{Wertheimer2020,
author = {Wertheimer, C M; Brandt, K; Kaminsky, S; Elhardt, C; Kassumeh, S A; Pham, L; Schulz-Hildebrandt, H; Priglinger, S; Anderson, R R and Birngruber, R},

url = {https://www.healio.com/ophthalmology/journals/jrs/2020-6-36-6/%7B8e6aaf40-922c-4998-9c7b-39b47135ec61%7D/refractive-changes-after-corneal-stromal-filler-injection-for-the-correction-of-hyperopia},
journal = {J Refractive Surg},
number = {6(36)},
pages = {406--413},
title = {Refractive Changes After Corneal Stromal Filler Injection for the Correction of Hyperopia},

date = {2020-06-14},
year = {2020},
keywords ={mOCT, HSH}
}

@article{Miura2020-2,
   author = {Yamamoto, M;Miura, Y;Hirayama, K;;Kohno, T;Kabata, D;Theisen-Kunde, D;Brinkmann, R and Honda, S;},
   title = {Predictive factors of outcome of selective retina therapy for diabetic macular edema},
   journal = {International Ophthalmology},
   ISSN = {1573-2630},
   
   url = {https://doi.org/10.1007/s10792-020-01288-6},
   year = {2020},
   type = {Journal Article}
}

@article{jackle2020,
title = {Three-dimensional guidance including shape sensing of a stentgraft system for endovascular aneurysm repair},
author = {Jäckle,S; Garcia-Vazquez,V; Eixmann, T; Matysiak, F; von Haxthausen,F; Sieren; M m; Schulz-Hildebrandt, H;  H\"{u}ttmann, G; Ernst, F; Kleemann, M and P\"{a}tz, T},
url = {https://doi.org/10.1007/s11548-020-02167-2},

isbn = {1861-6429},
year = {2020},
date = {2020-04-06},
journal = {Int J  Comp Assis Radiology and Surgery},
abstract = {During endovascular aneurysm repair (EVAR) procedures, medical instruments are guided with two-dimensional (2D) fluoroscopy and conventional digital subtraction angiography. However, this requires X-ray exposure and contrast agent is used, and the depth information is missing. To overcome these drawbacks, a three-dimensional (3D) guidance approach based on tracking systems is introduced and evaluated.},
keywords = {HSH},
pubstate = {published},
tppubtype = {article}
}

@article{Enzian2020,
   author = {Enzian, P.;Schell, C.;Link, A.;Malich, C.;Pries, R.;Wollenberg, B and Rahmanzadeh, R},
   title = {Optically controlled drug release from light-sensitive liposomes with the new photosensitizer 5,10-DiOH},
   journal = {Molecular Pharmaceutics},
   ISSN = {1543-8384},
   DOI = {10.1021/acs.molpharmaceut.9b01173},
   
   year = {2020},
   type = {Journal Article}
}

@article{Freidank2020,
   author = {Freidank, S;Vogel, A and Linz, N},
   title = {Optical Vortex Beam for Gentle and Ultraprecise Intrastromal Corneal Dissection in Refractive Surgery},
   journal = {TVST},
   volume = {9(10)},
   
   pages = {22-22},
   ISSN = {2164-2591},
 
   url = {https://doi.org/10.1167/tvst.9.10.22},
   year = {2020},
   type = {Journal Article}
}

@article{Palczewska-2020,
   title        = {Noninvasive two-photon optical biopsy of retinal fluorophores},
   author       = {Palczewska, G;Boguslawski, J;Stremplewski, P;Kornaszewski, L;Zhang, J;Dong, Z;Liang, Xiao-Xuan;Gratton, E;Vogel, A;Wojtkowski, M and Palczewski, K},
   year         = 2020,
   journal      = {Proceedings of the National Academy of Sciences},
   pages        = 202007527,
   doi          = {10.1073/pnas.2007527117},
   type         = {Journal Article}
}

@article{Kleymann2020,
   author = {Kleymann, V;Gernandt, H;Worthmann, K;Hossam, S.A;Brinkmann, R and Müller, A.M},
   title = {Modeling parameter for temperature controlled retinal laser therapies },
   journal = {DeGruyter-at-Automatisierungstechnik},
   volume = {68(11)},
keywords = {retinal photocoagulation, parametric model order
reduction, identification},
   pages = {953-966},
  URL = {https://www.degruyter.com/view/journals/auto/68/11/article-p953.xml},
   year = {2020},
   type = {Journal Article}
}

@article{pieper2020intravital,
title = {Intravital microscopic optical coherence tomography imaging to assess mucus mobilizing interventions for muco-obstructive lung disease in mice},
author = {Pieper,P; Schulz-Hildebrandt, H; Mall,Marcus A; H\"{u}ttmann, G and K\"{o}nig, P},
doi = {10.1152/ajplung.00287.2019},
year = {2020},
date = {2020-02-26},
journal = {American Journal of Physiology-Lung Cellular and Molecular Physiology},
volume = {318},
number = {3},
pages = {518-524},
publisher = {American Physiological Society Rockville, MD},
abstract = {Airway mucus obstruction is a hallmark of chronic lung diseases such as cystic fibrosis, asthma and COPD, and the development of more effective mucus mobilizing therapies remains an important unmet need for patients with these muco-obstructive lung diseases. However, methods for sensitive visualization and quantitative assessment of immediate effects of therapeutic interventions on mucus clearance in vivo are lacking. In this study, we determined if newly developed high-speed microscopic optical coherence tomography (mOCT) is sensitive to detect and compare in vivo effects of inhaled isotonic saline, hypertonic saline and bicarbonate on mucus mobilization and clearance in Scnn1b-transgenic mice with muco-obstructive lung disease. In vivomOCT imaging showed that inhaled isotonic saline-induced rapid mobilization of mucus that was mainly transported as chunks from the lower airways of Scnn1b-transgenic mice. Hypertonic saline mobilized a significantly greater amount of mucus that showed a more uniform distribution compared to isotonic saline. Addition of bicarbonate to isotonic saline had no effect on mucus mobilization, but also led to a more uniform mucus layer compared to treatment with isotonic saline alone. mOCT can detect differences in response to mucus mobilizing interventions in vivo, and may thus support the development of more effective therapies for patients with muco-obstructive lung diseases.},
keywords = {mOCT,OCT},
pubstate = {published},
tppubtype = {article}
}

@article{Fischer2020,
   author = {Fischer, T;Klinger, A;von Smolinski, D;Orzekowsky-Schroeder, R;Nitzsche, F;Bölke, T;Vogel, A;Hüttmann, G and Gebert, A},
   title = {High-resolution imaging of living gut mucosa: lymphocyte clusters beneath intestinal M cells are highly dynamic structures},
   journal = {Cell and Tissue Research},
   pages = {1-8},
   ISSN = {1432-0878},
   url = {https://doi.org/10.1007/s00441-020-03167-z},
   year = {2020},
   type = {Journal Article}
}

@article{Brinkmann2020,
   author = {Richert, E;Bartsch, S;Hillenkamp, J;Treumer, F;Tode, J;von der Burchard, C;Brinkmann, R;Klettner, A K and Roider, J},
   title = {Einfluss der Selektiven Retinatherapie (SRT) auf inflammatorische Zellmediatoren des subretinalen Raums},
   journal = {Klin Monatsbl Augenheilkd},
   volume = {237(02)},
   
   pages = {192-201},
   ISSN = {0023-2165},
   DOI = {10.1055/a-0838-5633},
   year = {2020},
   type = {Journal Article}
}

@article{Münter2020,
   author = {Münter, M;Endt, M v;Pieper, M;Casper, M;Ahrens, M;Kohlfaerber, T;Rahmanzadeh, R;König, P;Hüttmann, G and Schulz-Hildebrandt, H},
   title = {Dynamic contrast in scanning microscopic OCT},
   journal = {Optic Letters},
url = {https://arxiv.org/abs/2003.00006},
   year = {2020},
   type = {Journal Article}
}

@article{Holzhey2020,
   author = {Holzhey, A;Sonntag, S;Rendenbach, J;Ernesti, J S;Kakkassery, V;Grisanti, S;Reinholz, F;Freidank, S;Vogel, A and Ranjbar, M},
   title = {Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss},
   journal = {Journal of visualized experiments: JoVE},
   number = {158},
   ISSN = {1940-087X},
DOI = {10.3791/60542 },
   year = {2020},
   type = {Journal Article}
}

@article{Matkivsky2020,
   author = {Matkivsky, V;Moiseev, A;Shilyagin, P;Rodionov, A;Spahr, H;Pfäffle, C;Hüttmann, G;Hillmann, D and Gelikonov, G},
   title = {Determination and correction of aberrations in full field optical coherence tomography using phase gradient autofocus by maximizing the likelihood function},
   journal = {Journal of Biophotonics},
   volume = {13(10)},
   pages = {e202000112},
   ISSN = {1864-063X},
   DOI = {10.1002/jbio.202000112},
   year = {2020},
   type = {Journal Article}
}

@article{Kassumeh2020,
   author = {Kassumeh, S;Luther, J K.;Wertheimer, C M.;Brandt, K;Schenk, M S.;Priglinger, S G.;Wartak, A;Apiou-Sbirlea, G.;Anderson, R. R. and Birngruber, R},
   title = {Corneal Stromal Filler Injection as a Novel Approach to Correct Presbyopia—An Ex Vivo Pilot Study},
   journal = {TVST},
   volume = {9(7)},
  keywords = { presbyopia correction; corneal filler; refractive surgery; femtosecond laser; hyaluronic acid; bifocality},

   pages = {30-30},
   ISSN = {2164-2591},
   
   url = {https://doi.org/10.1167/tvst.9.7.30},
   year = {2020},
   type = {Journal Article}
}

@article{Schulz-Hildebrandt-2020,
   author = {Rakhymzhan, A;Reuter, L;Raspe, R;Bremer, D;Günther, R;Leben, R;Heidelin, J;Andresen, V;Cheremukhin, S;Schulz-Hildebrandt, H;Bixel, M G.;Adams, R H.;Radbruch, H;Hüttmann, G;Hauser, A E. and Niesner, R A.},
   title = {Coregistered Spectral Optical Coherence Tomography and Two-Photon Microscopy for Multimodal Near-Instantaneous Deep-Tissue Imaging},
  year = {2020},
   journal = {Cytometry Part A},
   volume = {97},
   number = {5},
   pages={515-527},
   ISSN = {1552-4922},
   DOI = {10.1002/cyto.a.24012},
   type = {Journal Article}
}

@article{brinkmann2020-2,
   author = {Luecking, M;Brinkmann, R;Ramos, Sc;Stork, W and Heussner, N},
   title = {Capabilities and limitations of a new thermal finite volume model for the evaluation of laser-induced thermo-mechanical retinal damage},
   journal = {CompBioMed},
   volume = {122},
   pages = {103835},
   ISSN = {0010-4825},
   DOI = {https://doi.org/10.1016/j.compbiomed.2020.103835},
 
   year = {2020},
   type = {Journal Article}
}

@article{Rahmanzadeh-2020,
   author = {Yao, C;Rudnitzki, F;He, Y;Zhang, Z;Hüttmann, G and Rahmanzadeh, R},
   title = {Cancer cell-specific protein delivery by optoporation with laser-irradiated gold nanorods},
   journal = {JBio},
   
   ISSN = {1864-063X},
   DOI = {10.1002/jbio.202000017},
  Year = {2020},
   type = {Journal Article}
}

@article{schneider2020,
   author = {Schneider, H.;Ahrens, M.;Strumpski, M.;Ruger, C.;Hafer, M.;Huettmann, G.;Theisen-Kunde, D.;Schulz-Hildebrandt, H. and Haak, R.},
   title = {An Intraoral OCT Probe to Enhanced Detection of Approximal Carious Lesions and Assessment of Restorations},
   journal = {J Clin Med},
   volume = {9(10)},
   keywords = {OCT; intraoral probe; carious lesions; caries diagnosis; dental restorations},
   ISSN = {2077-0383 (Print)
2077-0383 (Linking)},
   DOI = {10.3390/jcm9103257},
   
   year = {2020},
   type = {Journal Article}
}

@article{Koop2020,
   author = {Koop, N},
   title = {Beständig durch Mikrodefekte},
   journal = {Pharma+Food},
   url = { https://www.pharma-food.de/bestaendig-durch-mikrodefekte/},
   year = {2020},
   type = {Journal Article}
}

@proceedings{Horn2019b,
title = {First Steps into Catheter Guidance Including Shape Sensing for Endovascular Aneurysm Repair Procedures},
author = {Marco Horn and Sonja J\"{a}ckle and Felix von Haxthausen and Tim Eixmann and Hinnerk Schulz-Hildebrandt and Gereon H\"{u}ttmann and Juljan Bouchagiar and Florian Matysiak and Mark Kaschwich and Markus Kleemann and Floris Ernst and Ver\'{o}nica Garc\'{i}a-V\'{a}zquez and Torben P\"{a}tz},
doi = {10.1016/J.EJVS.2019.09.091},
issn = {1078-5884},
year  = {2019},
date = {2019-12-13},
journal = {European Journal of Vascular and Endovascular Surgery},
volume = {58},
number = {6},
pages = {e610--e611},
publisher = {W.B. Saunders},
keywords = {Sensing, Fiber},
pubstate = {published},
tppubtype = {proceedings}
}

@Proc{Enzian2019,
   author = {Enzian, P.;Link, A.;Schell, C.;Malich, C. and Rahmanzadeh, R.},
   title = {Light-induced permeabilization of liposomes},
   publisher = {Proc.SPIE},
   volume = {11070},
   series = {17th International Photodynamic Association World Congress},
   
   url = {https://doi.org/10.1117/12.2526071},
   year = {2019},
   type = {Book}
}

@article{RN5217,
   author = {Spahr, Hendrik;Pfäffle, Clara;Burhan, Sazan;Kutzner, Lisa;Hilge, Felix;Hüttmann, Gereon and Hillmann, Dierck},
   title = {Phase-sensitive interferometry of decorrelated speckle patterns},
   journal = {Scientific Reports},
   volume = {9},
   number = {1},
   pages = {11748},
   ISSN = {2045-2322},
   DOI = {10.1038/s41598-019-47979-8},
   url = {https://doi.org/10.1038/s41598-019-47979-8},
   year = {2019},
   type = {Journal Article}
}

@article{Lopez-Marin:19,
author = {Antonio L\'{o}pez-Mar\'{i}n and Geert Springeling and Robert Beurskens and Heleen van Beusekom and Antonius F. W. van der Steen and Arjun D. Koch and Brett E. Bouma and Robert Huber and Gijs van Soest and Tianshi Wang},
journal = {Opt. Lett.},
keywords = {Image reconstruction; Light beams; Magnetic fields; Optical coherence tomography; Optical imaging; Reflector design},
number = {15},
pages = {3641--3644},
publisher = {Optica Publishing Group},
title = {Motorized capsule for shadow-free OCT imaging and synchronous beam control},
volume = {44},
month = {Aug},
year = {2019},
url = {https://opg.optica.org/ol/abstract.cfm?URI=ol-44-15-3641},
doi = {10.1364/OL.44.003641},
abstract = {We demonstrate a tethered motorized capsule for unobstructed optical coherence tomography (OCT) imaging of the esophagus. By using a distal reflector design, we avoided the common shadow artifact induced by the motor wires. A synchronous driving technique features three types of beam-scanning modes of the capsule, i.e., circumferential beam scanning, localized beam scanning, and accurate beam positioning. We characterized these three modes and carried out ex vivo imaging experiments using the capsule. The results show that the capsule can potentially be a useful tool for diagnostic OCT imaging and OCT-guided biopsy and therapy of the esophagus.},
}

@article{Kolb2019,
   author = {Kolb, J P;Draxinger, W;Klee, J;Pfeiffer, T;Eibl, M;Klein, T;Wieser, W and Huber, R},
   title = {Live video rate volumetric OCT imaging of the retina with multi-MHz A-scan rates},
   journal = {J pone},
 keywords = {AG-Huber_OCT},
   url = {https://doi.org/10.1371/journal.pone.0213144},
   pages = {e0213144},
   ISSN = {1932-6203},
   
   year = {2019},
   type = {Journal Article}
}

@article{RN5085,
   author = {Spahr, H;Pfäffle, C;Hüttmann, G and Hillmann, D},
   title = {Artifacts in speckle tracking and multi-aperture
Doppler OCT imaging of lateral motion},
   journal = {Optics letters},
   volume = {44(5)},
   pages = {1315-1318},
   DOI = {https://doi.org/10.1364/OL.44001315},
   url = {https://doi.org/10.1364/OL.44001315},
   year = {2019},
   type = {Journal Article}
}

@article{Liang2019,
   author = {Liang, X-X; Zhang, Z and Vogel, A},
   title = {Multi-rate-equation modeling of the energy spectrum of laser-induced conduction band electrons in water},
   journal = {Opt Expr},
   
   
   pages = {4672-4693},
   DOI = {10.1364/OE.27.004672},
   
   year = {2019},
   type = {Journal Article}
}

@article{South:19,
author = { South,F A; Liu, Y-Z; Huang, P-C and Kohlfärber, T and Boppart, S A},
journal = { Opt Lett 44(5)},
pages = {1186--1189},
keywords = {Adaptive optics; Image metrics; Image quality; Refractive index; Speckle noise; Wavefront aberrations},
number = {5},
 
publisher = {OSA},
title = {Local wavefront mapping in tissue using computational adaptive optics OCT},


year = {2019},
doi = {10.1364/OL.44.001186},
abstract = {The identification and correction of wavefront aberrations is often necessary to achieve high-resolution optical images of biological tissues, as imperfections in the optical system and the tissue itself distort the imaging beam. Measuring the localized wavefront aberration provides information on where the beam is distorted and how severely. We have recently developed a method to estimate the single-pass wavefront aberrations from complex optical coherence tomography (OCT) data. Using this method, localized wavefront measurement and correction using computational OCT was performed in ex vivo tissues. The computationally measured wavefront varied throughout the imaged OCT volumes and, therefore, a local wavefront correction outperformed a global wavefront correction. The local wavefront measurement was also used to generate tissue aberration maps. Such aberration maps could potentially be used as a new form of tissue contrast.},
}

@article{Vogel2019-2,
    title        = {Materials synthesis in a bubble},
    author       = {Barcikowski, Stephan;Plech, Anton;Suslick, Kenneth S. and Vogel, Alfred},
    year         = 2019,
    journal      = {MRS Bulletin},
    volume       = {44 (5)},
    pages        = {382--391},
    doi          = {10.1557/mrs.2019.107},
    issn         = {0883-7694},
    type         = {Journal Article}
}

@article{Schmidt2019,
   author = {Schmidt, Mark;Pfeiffer, Tom;Grill, Christin;Huber, Robert and Jirauschek, Christian},
   title = {Modeling of the Ultra-Stable Operating Regime in Fourier Domain Mode Locked (FDML) Lasers},
   pages = {1-1},
   DOI = {10.1109/CLEOE-EQEC.2019.8873213},
   year = {2019},
   type = {Book},
booktitle = { 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference}
}

@article{Rahmanzadeh-2019,
   author = {Rahmanzadeh, R;Rudnitzki, F and Hüttmann, G},
   title = {Two ways to inactivate the Ki-67 protein—Fragmentation by nanoparticles, crosslinking with fluorescent dyes},
   journal = {Journal of Biophotonics},
   Year = {2019},
   pages = {e201800460},
   ISSN = {1864-063X},
   DOI = {10.1002/jbio.201800460},
   url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201800460},
   type = {Journal Article}
}

@article{Brinkmann2020,
   author = {Strittmatter, F;Eisel, M; Brinkmann, R; Cordes, J;Lange, B and Sroka, R},
   title = {Laser-induced lithotripsy: a review, insight into laboratory work, and lessons learned},
   journal = {Translational Biophotonics},
   volume = {n/a},
   number = {n/a},
   pages = {e201900029},
   ISSN = {2627-1850},
   DOI = {10.1002/tbio.201900029},
   url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/tbio.201900029},
   year = {2019},
   type = {Journal Article}
}

@article{Karpf2019,
   author = {Jiang, Y;Karpf, S and Jalali, B},
   title = {Time-stretch LiDAR as a spectrally scanned time-of-flight ranging camera},
   journal = {Nature Photonics},
   keywords={},
   ISSN = {1749-4893},
   URL = {https://www.researchgate.net/publication/337692338_Time-stretch_LiDAR_as_a_spectrally_scanned_time-of-flight_ranging_camera},
   year = {2019},
   type = {Journal Article}
}
 

@article{Spahr-2019,
   title        = {Phase-sensitive interferometry of decorrelated speckle patterns},
   author       = {Spahr, Hendrik;Pfäffle, Clara;Burhan, Sazan;Kutzner, Lisa;Hilge, Felix;Hüttmann, Gereon and Hillmann, Dierck},
   year         = 2019,
   journal      = {Scientific Reports},
   volume       = {9(1)},
   pages        = 11748,
   issn         = {2045-2322},
   url          = {https://doi.org/10.1038/s41598-019-47979-8},
   type         = {Journal Article}
}

@article{Miura2019/4,
   author = {Miura, Y;Seifert, E;Rehra, J;Kern, K;Theisen-Kunde, D;Denton, M and Brinkmann, R},
   title = {Real-time optoacoustic temperature determination on cell cultures during heat exposure: a feasibility study},
   journal = {Int J Hyperth},
   pages = {1-7},
   ISSN = {0265-6736},
  
   url = {https://doi.org/10.1080/02656736.2019.1590653},
   year = {2019},
   type = {Journal Article}
}

@article{Hüttmann2019,
   author = {Kepp, T;Droigk, C;Casper, M;Evers, M;Hüttmann, G;Salma, N;Manstein, D;Heinrich, M P. and Handels, H},
   title = {Segmentation of mouse skin layers in optical coherence tomography image data using deep convolutional neural networks},
   journal = {Biomed Opt Expr 10(7)},
  
   pages = {3484-3496},
   DOI = {10.1364/BOE.10.003484},
   url = {https://doi.org/10.1364/BOE.10.003484},
   year = {2019},
   type = {Journal Article}
}

@article{Pfäffle2019,
   author = {Pfäffle, C;Spahr, H;Kutzner, L;Burhan, S;Hilge, F;Miura, Y;Hüttmann, G and Hillmann, D},
   title = {Simultaneous functional imaging of neuronal and photoreceptor layers in living human retina},
   journal = {Optics Letters 44(23)},
 
   pages = {5671-5674},
   DOI = {10.1364/OL.44.005671},
   
   year = {2019},
   type = {Journal Article}
}

@article{Wang2019-2,
   author = {Wang, Tianshi;Pfeiffer, Tom;Daemen, Joost;Mastik, Frits;Wieser, Wolfgang;van der Steen, AFW;Huber, Robert and van Soest, Gijs},
   title = {Simultaneous morphological and flow imaging enabled by megahertz intravascular Doppler optical coherence tomography},
   journal = {IEEE Transactions on Medical Imaging},
   volume = {39},
   number = {5},
   pages = {1535-1544},
   ISSN = {0278-0062},
   year = {2019},
   type = {Journal Article}
}

@article{Tode2019,
   author = {Tode, J;von der Burchard, C;Richert, E;Klettner, A;Brinkman, R and Roider, J},
   title = {Thermische Stimulation der Retina: von der translationalen Forschung zur experimentellen technischen Umsetzung},
   journal = {Spitzenforschung in der Ophthalmologie},
   volume = {117.DOG Kongress},
   pages = {172-175},
   ISSN = {1861-4620},
   url = {https://www.dog.org/?cat=276},
   year = {2019},
   type = {Journal Article}
}

@article{
citation_key = { Casper19},
author = { Casper, M;  Schulz-Hildebrandt, H; Evers, M; Birngruber,R; Manstein, D and Hüttmann, G},
title = {Optimization-based vessel segmentation pipeline for robust quantification of capillary networks in skin with optical coherence tomography angiography},

journal = {J Biomed Opt},

pages = {046005 - 1 - 11},
year = {2019},

URL = {https://doi.org/10.1117/1.JBO.24.4.046005},
eprint = {}
}

@article{Bliedtner2019,
   author = {Bliedtner, K;Seifert, E and Brinkmann, R},
   title = {Towards Automatically Controlled Dosing for Selective Laser Trabeculoplasty},
   journal = {TVST 8(6)},
  
   ISSN = {2164-2591},
   DOI = {10.1167/tvst.8.6.24},
   year = {2019},
   type = {Journal Article}
}

@article{Kohlfärber2019,
   author = {Kohlfaerber, T;Ding, S;Rahmanzadeh, R;Jüngst, T;Groll, J;Schulz-Hildebrandt, H and Hüttmann, G},
   title = {Investigation of cell dynamics in 3D cell spheroids and cell interaction with 3D printed scaffolds by mOCT},
   journal = {Transactions on Additive Manufacturing Meets Medicine 1(1)},
   
   DOI = {10.18416/AMMM.2019.1909S03P19},
   year = {2019},
   type = {Journal Article}
}

@Article{Ourak2019,
author="Ourak, M.
and Smits, J.
and Esteveny, L.
and Borghesan, G.
and Gijbels, A.
and Schoevaerdts, L.
and Douven, Y.
and Scholtes, J.
and Lankenau, E.
and Eixmann, T.
and Schulz-Hildebrandt, H.
and H{\"u}ttmann, G.
and Kozlovszky, M.
and Kronreif, G.
and Willekens, K.
and Stalmans, P.
and Faridpooya, K.
and Cereda, M.
and Giani, A.
and Staurenghi, G.
and Reynaerts, D.
and Vander Poorten, E. B.",
title="Combined OCT distance and FBG force sensing cannulation needle for retinal vein cannulation: in vivo animal validation",
journal="International Journal of Computer Assisted Radiology and Surgery",
year="2019",
month="Feb",
day="01",
volume="14",
number="2",
pages="301--309",
abstract="Retinal vein cannulation is an experimental procedure during which a clot-dissolving drug is injected into an obstructed retinal vein. However, due to the fragility and minute size of retinal veins, such procedure is considered too risky to perform manually. With the aid of surgical robots, key limiting factors such as: unwanted eye rotations, hand tremor and instrument immobilization can be tackled. However, local instrument anatomy distance and force estimation remain unresolved issues. A reliable, real-time local interaction estimation between instrument tip and the retina could be a solution. This paper reports on the development of a combined force and distance sensing cannulation needle, and its experimental validation during in vivo animal trials.",
issn="1861-6429",
doi="10.1007/s11548-018-1829-0",
url="https://doi.org/10.1007/s11548-018-1829-0",
citation_key = {Ourak2019}
}

@article{von-der-Burchardt2019,
   author = {von der Burchard, C;Sudkamp, H;Tode, J;Ehlken, C;Moltmann, M;Münst, M;Theisen-Kunde, D;Koch, P;Hüttman, G and Roider, J},
   title = {High-Tech-Ophthalmologie zu Hause: Das Home-Care-OCT für AMD Patienten},
   journal = {Spitzenforschung in der Ophthalmologie- },
   pages = {30-32},
   ISSN = {1861-4620},
   url = {https://www.dog.org/?cat=276},
   year = {2019},
   type = {Journal Article}
}

@article{Spahr2019,
   author = {Spahr, H;Pfäffle, C;Huettmann, G and Hillmann, D},
   title = {Artifacts in speckle tracking and multi-aperture
Doppler OCT imaging of lateral motion},
   journal = {Optics letters},
   volume = {44(5)},
   pages = {1315-1318},
   DOI = {https://doi.org/10.1364/OL.44001315},
   url = {https://doi.org/10.1364/OL.44001315},
   year = {(2019)},
   type = {Journal Article}
}

@article{Spahr2019,
   author = {Spahr, H; Pfäffle, C; Huettmann, G and Hillmann, D},
   title = { Artifacts in speckle tracking and multi-aperture
Doppler OCT imaging of lateral motion},
   journal = { Opt Lett},
   pages = {1315-1318},
URL = {https://doi.org/10.1364/OL.44.001315},
   year = {2019},
   type = {Journal Article}
}

@article{Miura2019-2,
   author = {Rudolf, M; Curcio, C A; Schlözer-Schrehardt, U; Sefat, A M M; Tura, A; Aherrahrou, Z; Brinkmann, M; Grisanti, S;  Miura, Y and Ranjbar, M},
   title = {Apolipoprotein A-I mimetic peptide L-4F removes Bruch's membrane lipids in aged nonhuman primates},
   journal = {Invest Ophthalmol Vis Sci},
   pages = {461-472},  
   url = {https://www.ncbi.nlm.nih.gov/pubmed/30707219},
   year = {2019},
   type = {Journal Article}
}

@article{Casper2019b,
title = {Capillary Refill - The Key to Assessing Dermal Capillary Capacity and Pathology in Optical Coherence Tomography Angiography},
author = {Casper, M. J. and Glahn, J. and Evers, M. and Schulz-Hildebrandt, H. and Kositratna, G. and Birngruber, R. and H\"{u}ttmann, G. and Manstein, D.},
doi = {10.1002/lsm.23188},
year = {2019},
date = {2019-11-22},
journal = {Lasers in Surgery and Medicine},
abstract = {Background/Objectives Standard optical coherence tomography angiography (OCTA) has been limited to imaging blood vessels actively undergoing perfusion, providing a temporary picture of surface microvasculature. Capillary perfusion in the skin is dynamic and changes in response to the surrounding tissue's respiratory, nutritional, and thermoregulatory needs. Hence, OCTA often represents a given perfusion state without depicting the actual extent of the vascular network. Here we present a method for obtaining a more accurate anatomic representation of the surface capillary network in human skin using OCTA, along with proposing a new parameter, the Relative Capillary Capacity (RCC), a quantifiable proxy for assessing capillary dilation potential and permeability. Methods OCTA images were captured at baseline and after compression of the skin. Baseline images display ambient capillary perfusion, while images taken upon capillary refill display the network of existing capillaries at full capacity. An optimization-based automated vessel segmentation method was used to automatically analyze and compare OCTA image sequences obtained from two volunteers. RCC was then compared with visual impressions of capillary viability. Results Our OCTA imaging sequence provides a method for mapping cutaneous capillary networks independent of ambient perfusion. Differences between baseline and refill images clearly demonstrate the shortcomings of standard OCTA imaging and produce the RCC biometric as a quantifiable proxy for assessing capillary dilation potential and permeability. Conclusion Future dermatological OCTA diagnostic studies should implement the Capillary Refill Methods over standard imaging techniques and further explore the relevance of RCC to differential diagnosis and dermatopathology. 
\textbf{Lasers Surg. Med. © The Authors. Lasers in Surgery and Medicine published by Wiley Periodicals, Inc.}},
keywords = {mOCT},
pubstate = {published},
tppubtype = {article}
}

@article{Miura2019/3,
   
   author = {Miura, Y;Lewke, B;Hutfilz, A and Brinkmann, R},
   title = {Change in fluorescence lifetime of retinal pigment epithelium under oxidative stress},
   journal = {Nippon Ganka Gakkai Zasshi },
  
   pages = {105-114},
   url = {http://journal.nichigan.or.jp/Disp?style=abst&vol=123&year=2019&mag=0&number=2&start=105},
   year = {2019},
   type = {Journal Article}
}

@article{Miura2019,
   author = {Hirayama, K;Manabu Yamamoto, M; Takeya Kohno, T; Miura, Y; Brinkmann, R;  Shiraki,K;Theisen-Kunde, D; and Honda, S;},
   title = {Change in the Thickness of Retinal Layers after Selective Retina
Therapy (SRT) in Patients with Central Serous Chorioretinopathy},
   journal = {Osaka City Med.},
   volume = {65},
   pages = {55-63},
   url = {http://dlisv03.media.osaka-cu.ac.jp/il/meta_pub/G0000438repository_00306096-65-1-55},
   year = {2019},
   type = {Journal Article}
}

@article{Rahmanzadeh-2019-2,
   author = {Espinoza, S;Müller, M;Jenneboer, H;Peulen, L;Bradley, T;Purschke, M;Haase, M;Rahmanzadeh, R;Jüstel, T and Zwart, s},
   title = {Characterization of Micro- and Nanoscale LuPO4:Pr3+,Nd3+ with Strong UV-C Emission to Reduce X-Ray Doses in Radiation Therapy},
   journal = {Particle and Particle Systems Characterization},
   URL = {https://www.researchgate.net/publication/335364547_Characterization_of_Micro-_and_Nanoscale_LuPO4Pr3Nd3_with_Strong_UV-C_Emission_to_Reduce_X-Ray_Doses_in_Radiation_Therapy},
   year = {2019},
   type = {Journal Article}
}

@article{Rahmanzadeh2019,
   author = {Wang, S;Liu, H;Xin, J;Rahmanzadeh, R;Wang, J;Yao, C and Zhang, Z},
   title = {Chlorin-based photoactivable Galectin-3-inhibitor nanoliposome for enhanced photodynamic therapy and NK cell-related immunity in Melanoma},
   journal = {ACS Applied Materials & Interfaces},
   ISSN = {1944-8244},
   DOI = {10.1021/acsami.9b09560},  
   year = {2019},
   type = {Journal Article}
}

@article{Hillmann2019,
   author = {Hillmann, D.;Pfaffle, C.;Spahr, H.;Burhan, S.;Kutzner, L.;Hilge, F. and Hüttmann, G.},
   title = {Computational adaptive optics for optical coherence tomography using multiple randomized subaperture correlations},
   journal = {Opt Lett 44 (15)},
 
   pages = {3905-3908},
   ISSN = {1539-4794 (Electronic)
0146-9592 (Linking)},
   DOI = {10.1364/OL.44.003905},
   url = {https://www.ncbi.nlm.nih.gov/pubmed/31368998},
   year = {2019},
   type = {Journal Article}
}

@article{Freidank2019,
   author = {Freidank, S;Vogel, A;Anderson, R. R.;Birngruber, R and Linz, N},
   title = {Correction of hyperopia by intrastromal cutting and liquid filler injection},
   journal = {J Biomed Opt},
  
   number = {5%J Journal of Biomedical Optics},
   pages = {1-7, 7},
   
   url = {https://doi.org/10.1117/1.JBO.24.5.058001},
   year = {2019},
   type = {Journal Article}
}

@article{Lange2019,
   author = {Lange, B;Cordes, J. and Brinkmann, R},
   title = {Exploiting the aiming beam to increase the safety of laser lithotripsy: Experimental evaluation of light reflection and fluorescence},
   journal = {Lasers in Surgery and Medicine},
   
   ISSN = {0196-8092},
   DOI = {10.1002/lsm.23154},
   
   year = {2019},
   type = {Journal Article}
}

@article{Lange 2019,
   author = {Lange, B;Cordes, J. and Brinkmann, R},
   title = {Exploiting the aiming beam to increase the safety of laser lithotripsy: Experimental evaluation of light reflection and fluorescence},
   journal = {Lasers in Surgery and Medicine},
   ISSN = {0196-8092},   
   url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/lsm.23154},
   year = {2019},
   type = {Journal Article}
}

@article{Jäckle2019,
abstract = {Endovascular aortic repair procedures are currently conducted with 2D fluoroscopy imaging. Tracking systems based on fiber Bragg gratings are an emerging technology for the navigation of minimally invasive instruments which can reduce the X-ray exposure and the used contrast agent. Shape sensing of flexible structures is challenging and includes many calculations steps which are prone to different errors. To reduce this errors, we present an optimized shape sensing model.},
author = {J{\"{a}}ckle, S; Eixmann, T; Schulz-Hildebrandt, H; H{\"{u}}ttmann, Gereon and P{\"{a}}tz, T},

file = {:Users/schulz-hildebrandt/Documents/Mendeley Desktop/J{\"{a}}ckle et al/International Journal of Computer Assisted Radiology and Surgery/J{\"{a}}ckle et al.{\_}2019{\_}Fiber optical shape sensing of flexible instruments for endovascular navigation.pdf:pdf},
issn = {1861-6429},
journal = {International Journal of Computer Assisted Radiology and Surgery},
month = {sep},
year = {2019},
title = {{Fiber optical shape sensing of flexible instruments for endovascular navigation}},
url = {https://doi.org/10.1007/s11548-019-02059-0},
keywords = {Endoskope, meos},

}

@article{Hutfilz2019,
   author = {Hutfilz, A;Sonntag, S;Lewke, B;Theisen-Kunde, D;Grisanti, S;Brinkmann, R and Miura, Y},
   title = {Fluorescence Lifetime Imaging Ophthalmoscopy of the Retinal Pigment Epithelium During Wound Healing After Laser Irradiation},
   journal = {Translational Vision Science & Technology},
   volume = {8(5)},
 
   ISSN = {2164-2591},
   DOI = {10.1167/tvst.8.5.12},
   year = {2019},
   type = {Journal Article}
}

@article{Karpf2019,
   author = {Karpf, S and Jalali, B },
   title = {Fourier-domain mode-locked laser combined with a master-oscillator power amplifier architecture},
   journal = {J Opt Lett},
   URL = {https://doi.org/10.1364/OL.44.001952},
   pages = {1952-1955},
   ISSN = {1539-4794},
   year = {2019},
 keywords = {},
   type = {Journal Article}
}

@article{karpf2019-2,
   author = {Karpf, S and Jalali, B},
   title = {Frequency-doubled FDML-MOPA laser in the visible},
   journal = {Opt Lett 44(24)},
   keywords = {},
   pages = {5913-5916},
   DOI = {10.1364/OL.44.005913},
   
   year = {2019},
   type = {Journal Article}
}

@article{Vogel-2019-1,
   author = {Fischer, T;Klinger, A;Smolinski, D von;Orzekowsky-Schroeder, R;Nitzsche, F;Vogel, A;Hüttmann, G and Gebert, A},
   title = {High-resolution imaging of the living gut mucosa: lymphocyte clusters beneath intestinal M cells are highly dynamic structures},
   journal = {Cell and Tissue Research},
   ISSN = {0302-766X (Print) 
1432-0878 (Online)},
   year = {2019},
   type = {Journal Article}
}

@article{Maertz2018,
   author = {Maertz, J; Kolb, J P; Klein, T; Mohler, K J; Eibl, M; Wieser, W; Huber, R; Priglinger, S and Wolf, A},
   title = {Combined in-depth, 3D, en face imaging of the optic disc, optic disc pits and optic disc pit maculopathy using swept-source megahertz OCT at 1050 nm},
   journal = {Graefe's Archive for Clinical and Experimental Ophthalmology},
   number = {2},
   pages = {289-298},
   DOI = {10.1007/s00417-017-3857-9},
   url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032262413&doi=10.1007%2fs00417-017-3857-9&partnerID=40&md5=a46c315f12cf5e633ea0f7e644116eb3},
   year = {2018},
   Keywords= {En face imaging, Optical coherence tomography, Swept-source OCT, Megahertz OCT, 3D rendering, Optic disc, Optic disc pit, Optic disc pit maculopathy, AG-Huber_OCT},
   type = {Journal Article}
}

@article{seifert2018,
   author = {Seifert, E; Tode, J; Pielen, A; Theisen-Kunde, D; Framme, C; Roider, J; Miura, Y; Birngruber, R and Brinkmann, R},
   title = {Selective retina therapy: toward an optically controlled automatic dosing},
   journal = {J Biomed Opt},
   
   pages = {1-12},
   ISSN = {1560-2281 (Electronic)
1083-3668 (Linking)},
   DOI = {10.1117/1.JBO.23.11.115002},   
keywords = {algorithm, lasers in medicine, ophthalmology, retinal pigment epithelium, selective retina therapy, selectivity},
   year = {2018},
   type = {Journal Article}
}

@article{Eibl2018,
  doi = {10.1364/boe.9.006273},
  url = {https://doi.org/10.1364/boe.9.006273},
  year = {2018},
  month = nov,
  publisher = {The Optical Society},
  volume = {9},
  number = {12},
  pages = {6273},
  author = {Matthias Eibl and Daniel Weng and Hubertus Hakert and Jan Philip Kolb and Tom Pfeiffer and Jennifer E. Hundt and Robert Huber and Sebastian Karpf},
  title = {Wavelength agile multi-photon microscopy with a fiber amplified diode laser},
  journal = {Biomedical Optics Express}
}

@article{Schulz-Hildebrandt:18,
author = {Hinnerk Schulz-Hildebrandt and Tom Pfeiffer and Tim Eixmann and Sabrina Lohmann and Martin Ahrens and Joshua Rehra and Wolfgang Draxinger and Peter K\"{o}nig and Robert Huber and Gereon H\"{u}ttmann},
journal = {Opt. Lett.},
keywords = {Fiber optics imaging; Endoscopic imaging; Medical and biological imaging; Optical coherence tomography; Fourier domain mode locking; Image quality; Optical coherence tomography; Single mode fibers; Step index fibers; Three dimensional imaging},
number = {18},
pages = {4386--4389},
publisher = {Optica Publishing Group},
title = {High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography},
volume = {43},
month = {Sep},
year = {2018},
url = {https://opg.optica.org/ol/abstract.cfm?URI=ol-43-18-4386},
doi = {10.1364/OL.43.004386},
abstract = {We present a forward-viewing fiber scanning endoscope (FSE) for high-speed volumetric optical coherence tomography (OCT). The reduction in size of the probe was achieved by substituting the focusing optics by an all-fiber-based imaging system which consists of a combination of scanning single-mode fibers, a glass spacer, made from a step-index multi-mode fiber, and a gradient-index fiber. A lateral resolution of 11 $\mu$m was achieved at a working distance of 1.2 mm. The newly designed piezo-based FSE has an outer diameter of 1.6 mm and a rigid length of 13.5 mm. By moving the whole imaging optic in spirals for scanning the sample, the beam quality remains constant over the entire field of view with a diameter of 0.8 mm. The scanning frequency was adjusted to 1.22 kHz for use with a 3.28 MHz Fourier domain mode locked OCT system. Densely sampled volumes have been imaged at a rate of 6 volumes per second.},
}

@article{Pfeiffer:18,
author = {Tom Pfeiffer and Markus Petermann and Wolfgang Draxinger and Christian Jirauschek and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {Fiber optics imaging; Lasers, fiber; Optical coherence tomography; Laser stabilization ; Lasers, frequency modulated ; Analog to digital converters; Dark solitons; Image quality; Laser modes; Mode locking; Optical coherence tomography},
number = {9},
pages = {4130--4148},
publisher = {Optica Publishing Group},
title = {Ultra low noise Fourier domain mode locked laser for high quality megahertz optical coherence tomography},
volume = {9},
month = {Sep},
year = {2018},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-9-9-4130},
doi = {10.1364/BOE.9.004130},
abstract = {We investigate the origin of high frequency noise in Fourier domain mode locked (FDML) lasers and present an extremely well dispersion compensated setup which virtually eliminates intensity noise and dramatically improves coherence properties. We show optical coherence tomography (OCT) imaging at 3.2 MHz A-scan rate and demonstrate the positive impact of the described improvements on the image quality. Especially in highly scattering samples, at specular reflections and for strong signals at large depth, the noise in optical coherence tomography images is significantly reduced. We also describe a simple model that suggests a passive physical stabilizing mechanism that leads to an automatic compensation of remaining cavity dispersion in FDML lasers.},
}

@article{Sudkamp2018a,
   author = {Sudkamp, H; Hillmann, D; Koch, P;vom Endt, M; Spahr, H; Münst, M; Pfäffle, C; Birngruber, R and Hüttmann, G},
   title = {Simple approach for aberration-corrected OCT imaging of the human retina},
   journal = {Opt Lett},
   
   pages = {4224},
   ISSN = {0146-9592
1539-4794},
   DOI = {10.1364/ol.43.004224},
   year = {2018},
   type = {Journal Article},
   keyword = {Retome}
}

@article{Schmidt:20,
author = {Mark Schmidt and Tom Pfeiffer and Christin Grill and Robert Huber and Christian Jirauschek},
journal = {OSA Continuum},
keywords = {Doppler effect; Laser modes; Laser sources; Nonlinear effects; Stimulated Raman scattering; Vertical cavity surface emitting lasers},
number = {6},
pages = {1589--1607},
publisher = {Optica Publishing Group},
title = {Self-stabilization mechanism in ultra-stable Fourier domain mode-locked (FDML) lasers},
volume = {3},
month = {Jun},
year = {2020},
url = {https://opg.optica.org/osac/abstract.cfm?URI=osac-3-6-1589},
doi = {10.1364/OSAC.389972},
abstract = {Understanding the dynamics of Fourier domain mode-locked (FDML) lasers is crucial for determining physical coherence limits, and for finding new superior methods for experimental realization. In addition, the rich interplay of linear and nonlinear effects in a laser ring system is of great theoretical interest. Here we investigate the dynamics of a highly dispersion-compensated setup, where over a bandwidth of more than 100\&\#x2009;nm, a highly coherent output with nearly shot-noise-limited intensity fluctuations was experimentally demonstrated. This output is called the sweet-spot. We show by numerical simulation that a finite amount of residual dispersion in the fiber delay cavity of FDML lasers can be compensated by the group delay dispersion in the swept bandpass filter, such that the intensity trace exhibits no dips or high-frequency distortions, which are the main source of noise in the laser. In the same way, a small detuning from the ideal sweep filter frequency can be tolerated. Furthermore, we find that the filter\&\#x2019;s group delay dispersion improves the coherence properties of the laser, and acts as a self-stabilizing element in the cavity. Our theoretical model is validated against experimental data, showing that all relevant physical effects for the sweet-spot operating regime are included.},
}

@article{Kolb:18,
author = {Jan Philip Kolb and Tom Pfeiffer and Matthias Eibl and Hubertus Hakert and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {Medical optics instrumentation; Lasers, fiber; Medical and biological imaging; Ophthalmic optics and devices ; Optical coherence tomography; Adaptive optics; Image quality; In vivo imaging; Mode locking; Ophthalmic imaging; Three dimensional imaging},
number = {1},
pages = {120--130},
publisher = {Optica Publishing Group},
title = {High-resolution retinal swept source optical coherence tomography with an ultra-wideband Fourier-domain mode-locked laser at MHz A-scan rates},
volume = {9},
month = {Jan},
year = {2018},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-9-1-120},
doi = {10.1364/BOE.9.000120},
abstract = {We present a new 1060 nm Fourier domain mode locked laser (FDML laser) with a record 143 nm sweep bandwidth at 2\&\#x2219;\&\#x202F;417 kHz\&\#x202F; $=$ \&\#x202F;834 kHz and 120 nm at 1.67 MHz, respectively. We show that not only the bandwidth alone, but also the shape of the spectrum is critical for the resulting axial resolution, because of the specific wavelength-dependent absorption of the vitreous. The theoretical limit of our setup lies at 5.9 \&\#x00B5;m axial resolution. In vivo MHz-OCT imaging of human retina is performed and the image quality is compared to the previous results acquired with 70 nm sweep range, as well as to existing spectral domain OCT data with 2.1 \&\#x00B5;m axial resolution from literature. We identify benefits of the higher resolution, for example the improved visualization of small blood vessels in the retina besides several others.},
}

@article{Tode2018,
   author = {Tode, J; Richert, E; Koinzer, S; Klettner, A; von der Burchard, C; Brinkmann, R; Lucius, R and Roider, J},
   title = {Thermal Stimulation of the Retina Reduces Bruch's Membrane Thickness in Age Related Macular Degeneration Mouse Models},
   journal = {Transl Vis Sci Technol},
  
   pages = {2},
   ISSN = {2164-2591 (Print)
2164-2591 (Linking)}, 
   url = {https://www.ncbi.nlm.nih.gov/pubmed/29736323},
   year = {2018},
   type = {Journal Article}
}

@article{Spahr:18,
author = { Spahr, H; Pfäffle, C; Koch,C; Sudkamp, H; Hüttmann, G und Hillmann, D},
journal = {Opt Expr},
keywords = {Funktion, Fullfield},

pages = {18803--18816},
publisher = {OSA},
title = {Interferometric detection of 3D motion using computational subapertures in optical coherence tomography},


year = {2018},
url = {https://doi.org/10.1364/OE.26.018803},

abstract = {Doppler optical coherence tomography (OCT) quantifies axial motion with high precision, whereas lateral motion cannot be detected by a mere evaluation of phase changes. This problem was solved by the introduction of three-beam Doppler OCT, which, however, entails a high experimental effort. Here, we present the numerical analogue to this experimental approach. Phase-stable complex-valued OCT datasets, recorded with full-field swept-source OCT, are filtered in the Fourier domain to limit imaging to different computational subapertures. These are used to calculate all three components of the motion vector with interferometric precision. As known from conventional Doppler OCT for axial motion only, the achievable accuracy exceeds the actual imaging resolution by orders of magnitude in all three dimensions. The feasibility of this method is first demonstrated by quantifying micro-rotation of a scattering sample. Subsequently, a potential application is explored by recording the 3D motion vector field of tissue during laser photocoagulation in ex-vivo porcine retina.},
}

@article{Strauch2018,
   author = {Strauch, M;Somers, P A. A. M.;Bociort, F and Urbach, H. P},
   title = {Creation of aspheric interfaces on an electrowetting liquid lens using surface oscillations},
   journal = {AIP Advances},
   
   ISSN = {2158-3226},
   DOI = {10.1063/1.5063994},
   year = {2018},
   type = {Journal Article}
}

@article{Evers2018,
   author = {Evers, M ;Salma, N; Osseiran, S; Casper, M; Birngruber, R; Evans, C L and Manstein, D},
   title = {Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM},
   journal = {Scientific Reports},
   
   DOI = {10.1038/s41598-018-27093-x},
   
   year = {2018},
   type = {Journal Article}
}

@article{Brinkmann2018,
   author = {Han, J W; Choi, J; Kim, Y S, Kim, J; Brinkmann, R; Lyu, J and Park, T K},
   title = {Comparison of the neuroinflammatory responses to selective retina therapy and continuous-wave laser photocoagulation in mouse eyes},
   journal = {Graefe's Archive for Clinical and Experimental Ophthalmology},
   
   pages = {341-353},
 
URL= {https://doi.org/10.1007/s00417-017-3883-7},
   year = {2018},
   type = {Journal Article}
}

@article{Rahmanzadeh2018,
   author = {Rudnitzki, F; Feineis, S; Rahmanzadeh, R; Endl, E; Lutz, J; Groll, J and Huettmann, G},
   title = {siRNA release from gold nanoparticles by nanosecond pulsed
laser irradiation and analysis of the involved temperature
increase},
   journal = {Journal of BIOPHOTONICS},
  doi = {10.1002/jbio.201700329},
   

keywords = {cavitation|cell manipulation|controlled release|gold nanoparticle bio-conjugates|laser nanoeffects},
abstract = {Nanosecond pulsed laser irradiation can trigger a release of nucleic acids from gold nanoparticles, but the involved nanoeffects are not fully understood yet. Here we investigate the release of coumarin labeled siRNA from 15 to 30 nm gold particles after nanosecond pulsed laser irradiation. Temperatures in the particle and near the surface were calculated for the different radiant exposures. Upon irradiation with laser pulses of 4 nanosecond duration release started for both particle sizes at a calculated temperature increase of approximately 500 K. Maximum coumarin release was observed for 15 nm particles after irradiation with radiant exposure of 80 mJ cm−2 and with 32 mJ cm−2 for 30 nm particles. This corresponds to a temperature increase of 815 and 900 K, respectively. Our results show that the molecular release by nanosecond pulsed irradiation is based on a different mechanism compared to continuous or femtosecond irradiation. Local temperatures are considerably higher and it is expected that bubble formation plays a crucial role in release and damage to cellular structures. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim},
   year = {2018},
   type = {Journal Article}
}

@article{borghesan2018single,
title = {Single Scan OCT-based Retina Detection for Robot-assisted Retinal Vein Cannulation},
author = {Borghesan,G; Ourak,M; Lankenau, E; H\"{u}ttmann,G; Schulz-Hildebrandt,H; Willekens,K; Stalmans, K; Reynaerts, D and Vander Poorten,E},
editor = {World Scientific},
url = {https://www.worldscientific.com/doi/abs/10.1142/S2424905X18400056},
doi = {10.1142/S2424905X18400056},
year = {2018},
date = {2018-02-12},
journal = {J Med Robot Res},

pages = {184005},
abstract = {Vitreoretinal surgery concerns a set of particularly demanding minimal invasive micro-surgical interventions at the retina. Micro-surgeons are targeting sub-millimeter-sized structures here. Tiny vessels or wafer-thin membranes are to be cannulated or need to be peeled off. The greatest care is to be displayed not to damage these fragile structures or to inadvertently injure the underlying retina. Damage to the latter is mostly irreparable and might cause permanent loss of vision. Despite the availability over excellent stereo microscopes, wide-angle lenses and powerful light source visualization remains a problem. Especially, the limited depth perception is still perceived as a major bottle-neck whereas efforts have been conducted to integrate sensing capability in today’s state-of-the-art instruments, so far, little effort has been paid to process the obtained sensor data and turns this into a reliable source of information upon which robot assistive guidance schemes could be endowed upon. This paper proposes a number of processing techniques tailored to Optical Coherence Tomography (OCT) measurements. The first results of the proposed algorithms show that it is feasible to extract good and reliable distance estimates from this otherwise rather noisy signal and from a fairly limited dataset. The used data are the so-called A-scans. These are OCT measurements consisting of a single-line image that could be captured by an instrument-mounted fiber through which the OCT signal passes back-and-forth. However, in this work, we perform a pilot study whereby the employed A-scans are extracted from B-scans that are captured by a microscope-mounted OCT scanner, rather than obtained from a probe. The performance of a first embodiment of the algorithm that is based on an Unscented Kalman Filter (UKF) is compared to the performance of a second embodiment that relies on a Particle Filter (PF), focusing on the issues in filter initialization and the tracking quality. Finally, results of UKF and PF executions on a validation dataset are presented. 
Read More: https://www.worldscientific.com/doi/abs/10.1142/S2424905X18400056},
keywords = {Endoskope},
pubstate = {published},
tppubtype = {article}
}

@article{Miura2018,
   author = {Kern, K; Mertineit, C L; Brinkmann, R and Miura, Y},
   title = {Expression of heat shock protein 70 and cell death kinetics after different thermal impacts on cultured retinal pigment epithelial cells},
   journal = {Exp Eye Res},
  
   pages = {117-126},
   ISSN = {1096-0007 (Electronic)
0014-4835 (Linking)},
   DOI = {10.1016/j.exer.2018.02.013},
   year = {2018},
   type = {Journal Article}
}

@article{Brinkmann2018,
   author = {Richert, E; Koinzer, S; Tode, J; Schlott, K; Brinkmann, R; Hillenkamp, J; Klettner, A and Roider, J},
   title = {Release of Different Cell Mediators During Retinal Pigment Epithelium Regeneration Following Selective Retina Therapy},
   journal = {Investigative Ophthalmology & Visual Science},
   
   pages = {1323-1331},
   ISSN = {1552-5783},
  
   url = {http://dx.doi.org/10.1167/iovs.17-23163},
   year = {2018},
   type = {Journal Article}
}

@article{schulz2018novel,
title = {Novel endoscope with increased depth of field for imaging human nasal tissue by microscopic optical coherence tomography},
author = {Schulz-Hildebrandt, H; Pieper, M; Stehmar,C; Ahrens, M; Idel, C; Wollenberg,B; K\"{o}nig,P and Gereon H\"{u}ttmann},
editor = {Optic Socie Amer},
url = {https://www.osapublishing.org/abstract.cfm?URI=boe-9-2-636
https://www.osapublishing.org/boe/viewmedia.cfm?uri=boe-9-2-636&seq=0},
doi = {10.1364/BOE.9.000636},
isbn = {10.1364/BOE.9.000636},
year = {2018},
date = {2018-01-16},
journal = {Biomedical Optics Express},

pages = {636-647},
abstract = {Intravital microscopy (IVM) offers the opportunity to visualize static and dynamic changes of tissue on a cellular level. It is a valuable tool in research and may considerably improve clinical diagnosis. In contrast to confocal and non-linear microscopy, optical coherence tomography (OCT) with microscopic resolution (mOCT) provides intrinsically cross-sectional imaging. Changing focus position is not needed, which simplifies especially endoscopic imaging. For in-vivo imaging, here we are presenting endo-microscopic OCT (emOCT). A graded-index-lens (GRIN) based 2.75 mm outer diameter rigid endoscope is providing 1.5 \textendash 2 μm nearly isotropic resolution over an extended field of depth. Spherical and chromatic aberrations are used to elongate the focus length. Simulation of the OCT image formation, suggests overall a better image quality in this range compared to a focused Gaussian beam. Total imaging depth at a reduced sensitivity and lateral resolution is more than 200 μm. Using a frame rate of 80 Hz cross-sectional images of concha nasalis were demonstrated in humans, which could resolve cilial motion, cellular structures of the epithelium, vessels and blood cells. Mucus transport velocity was determined successfully. The endoscope may be used for diagnosis and treatment control of different lung diseases like cystic fibrosis or primary ciliary dyskinesia, which manifest already at the nasal mucosa.},
keywords = {Endoskope, OCM},
pubstate = {published},
tppubtype = {article}
}

@article{Baade2017,
   author = {Baade, A; von der Burchard, C; Lawin, M; Koinzer, S; Schmarbeck, B; Schlott, K; Miura, Y; Roider, J; Birngruber, R and Brinkmann, R},
   title = {Power-controlled temperature guided retinal laser therapy},
   journal = {J Biomed Opt},
   
   pages = {1-11},
   ISSN = {1083-3668},
   DOI = {10.1117/1.jbo.22.11.118001},
   year = {2017},
   type = {Journal Article}
}

@article{Eibl:17,
author = {Matthias Eibl and Sebastian Karpf and Hubertus Hakert and Torben Bl\"{o}mker and Jan Philip Kolb and Christian Jirauschek and Robert Huber},
journal = {Opt. Lett.},
keywords = {Lasers, fiber; Lasers, Raman; Nonlinear optics, four-wave mixing; Scattering, stimulated Raman; Lasers, ytterbium ; Fiber lasers; Master oscillator power amplifiers; Nanosecond pulses; Raman scattering; Stimulated Brillouin scattering; Wavelength conversion},
number = {21},
pages = {4406--4409},
publisher = {Optica Publishing Group},
title = {Pulse-to-pulse wavelength switching of a nanosecond fiber laser by four-wave mixing seeded stimulated Raman amplification},
volume = {42},
month = {Nov},
year = {2017},
url = {https://opg.optica.org/ol/abstract.cfm?URI=ol-42-21-4406},
doi = {10.1364/OL.42.004406},
abstract = {We report on a multi-color fiber laser based on four-wave mixing (FWM) and stimulated Raman scattering (SRS), delivering rapidly wavelength switchable narrowband output at 1064, 1122, and 1186\&\#x00A0;nm. High-power pulses from a nanosecond pulsed fiber master oscillator power amplifier at 1064\&\#x00A0;nm are combined with 1122\&\#x00A0;nm of seed light for Raman amplification at the first Stokes order in a standard single-mode fiber. With increasing power, we observe a narrowband spectral component at 1186\&\#x00A0;nm, without any additional seed or resonator at this wavelength. We analyze this occurrence of a narrowband second Stokes order both experimentally and theoretically and suggest it is a result of FWM seeding of the SRS amplification in the fiber. We demonstrate that the wavelength shifting can be controlled electronically within microseconds for very rapid and even pulse-to-pulse wavelength changes. This wavelength conversion method can extend the spectral coverage of single-wavelength fiber lasers for biomedical imaging.},
}

@article{Kretschmer2017,
   author = {Kretschmer, S; Pieper, M; Klinger, A; Hüttmann, G. and König, P.},
   title = {Imaging of Wound Closure of Small Epithelial Lesions in the Mouse Trachea},
   journal = {Am J Pathol},
   ISSN = {0002-9440},
   DOI = {10.1016/j.ajpath.2017.07.006},
 
   year = {2017},
pages = {2451-2460},
   type = {Journal Article}
}

@article{Hillmann2017,
   author = {Hillmann, D; Spahr, H; Sudkamp, H; Hain, C; Hinkel, L; Franke, G and Hüttmann, G},
   title = {Off-axis reference beam for full-field swept-source OCT and holoscopy},
   journal = {Opt Expr},
   
   pages = {27770-27784},
   DOI = {10.1364/OE.25.027770},
   year = {2017},
   type = {Journal Article}
}

@article{Miura2017,
   author = { Ataka,S;Ogawa,S; Miura,Y; Kohno,T and Shiraki, K},
   title = {A Comparison of Intraoperative Metrics between the Infiniti with and the Centurion without Intelligent Phacoemulsification Systems},
   journal = {Journal of Eye & Cataract Surgery},
   volume = {3},
   ISSN = {2571-8300},
Abstract = {Purpose: To compare the efficiency and safety of two
phacoemulsification systems, the Infiniti with intelligent
phacoemulsification (IP) and the Centurion without IP, in
terms of intraoperative metrics of phacoemulsification and
the occurrences of adverse events and intraoperative tip
obstruction.
Design: Comparative case series.
Methods: A consecutive series of 340 eyes (151 eyes with
the Infiniti, 189 eyes with the Centurion) was evaluated. A
2.4 mm sclerocorneal incision was used with the Infiniti, and
a 2.0 mm incision was used with the Centurion.
Phacoemulsification of the nucleus was performed using
the phaco chop technique, with the IP function of the
Infiniti turned on and the IP mode of the Centurion turned
off. From among the parameters displayed on the panels of
both devices, ultrasound (US) time, cumulative dissipated
energy (CDE), aspiration time, and estimated balanced salt
solution (BSS) aspiration volume were investigated and
compared between the devices. Results: A comparison of all
subjects found significant differences between the two
devices in mean CDE (p=0.02) and mean aspiration time
(p=0.003), but not in mean US time (p=0.43) or mean
estimated BSS aspiration volume (p=0.07). For grade 3
nuclei, all parameters of mean US time (p=0.0044), mean
CDE (p ≤ 0.001), mean aspiration time (p<0.001), and mean
estimated BSS aspiration volume (p=0.001) showed
significant differences favoring the Centurion.
Conclusions: Compared to the Infiniti with IP mode, cataract
surgery with the Centurion without IP mode is likely to be
performed with less phacoemulsification energy and higher
efficiency.},
   DOI = {10.21767/2471-8300.100034},
   year = {2017},
   type = {Journal Article}
}

@article{Maertz2017,
   author = {Maertz, J. and Mohler, K. J. and Kolb, J. P. and Klein, T. and Neubauer, A. and Kampik, A. and Priglinger, S. and Wieser, W. and Huber, R. and Wolf, A.},
   title = {INTRAPAPILLARY PROLIFERATION IN OPTIC DISK PITS: Clinical Findings and Time-Related Changes},
   journal = {Retina},
   volume = {37},
   number = {5},
   pages = {906-914},
   DOI = {10.1097/iae.0000000000001260},
   year = {2017},
keywords = {AG-Huber_OCT},
   type = {Journal Article}
}

@article{Karpf2017,
   author = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
   title = {Shot-Noise Limited Time-Encoded Raman Spectroscopy},
   journal = {Journal of Spectroscopy},
   volume = {2017},
   pages = {1-6},
   url = {https://doi.org/10.1155/2017/9253475},
   year = {2017},
keywords = {AG-Huber_NL},
   type = {Journal Article}
}

@article{Pfäffle2017,
   author = {Pfäffle, Clara and Spahr, Hendrik and Hillmann, Dierck and Sudkamp, Helge and Franke, Gesa and Koch, Peter and Hüttmann, Gereon},
   title = {Reduction of frame rate in full-field swept-source optical coherence tomography by numerical motion correction [Invited]},
   journal = {Biomedical Optics Express},
   volume = {8},
   number = {3},
   pages = {1499-1511},
   keywords = {Image reconstruction-restoration
Optical coherence tomography},
   url = {http://www.osapublishing.org/boe/abstract.cfm?URI=boe-8-3-1499},
   year = {2017},
   type = {Journal Article}
}

@article{Klein:17,
author = {Thomas Klein and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {Imaging systems; Optical coherence tomography; Lasers and laser optics; Lasers, tunable; Optical coherence tomography; Full field optical coherence tomography; High speed imaging; Image quality; Imaging systems; Light wavelength; X ray imaging},
number = {2},
pages = {828--859},
publisher = {Optica Publishing Group},
title = {High-speed OCT light sources and systems \[Invited\]},
volume = {8},
month = {Feb},
year = {2017},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-8-2-828},
doi = {10.1364/BOE.8.000828},
abstract = {Imaging speed is one of the most important parameters that define the performance of optical coherence tomography (OCT) systems. During the last two decades, OCT speed has increased by over three orders of magnitude. New developments in wavelength-swept lasers have repeatedly been crucial for this development. In this review, we discuss the historical evolution and current state of the art of high-speed OCT systems, with focus on wavelength swept light sources and swept source OCT systems.},
}

@article{Strauch2017,
   author = {Strauch, M;Shao, Y;Bociort, F and Urbach, H. P},
   title = {Study of surface modes on a vibrating electrowetting liquid lens},
journal = {Applied physics Letters},

  
   pages = {171106},
   DOI = {10.1063/1.4999562},
keywords = {lenses,surface waves(fluid),vibrations,wetting},
abstract = {The increased usage of liquid lenses motivates us to investigate surface waves on the liquid's surface. During fast focal switching, the surface waves decrease the imaging quality. We propose a model that describes the surface modes appearing on a liquid lens and predicts the resonance frequencies. The effects of those surface modes on a laser beam are simulated using Fresnel propagation, and the model is verified experimentally.
We acknowledge the use of an educational licence of CODE V and LightTools. This work was funded through the Spectr@phone project (IPD 12017) of the IOP Photonic Devices program of RVO.},

   url = {https://aip.scitation.org/doi/abs/10.1063/1.4999562},
   year = {2017},
   type = {Journal Article}
}

@article{Lange2017,
   author = {Lange, Birgit and Jocham, Dieter and Brinkmann, Ralf and Cordes, Jens},
   title = {Stone/tissue differentiation for Holmium laser lithotripsy using autofluorescence: Clinical proof of concept study},
   journal = {Lasers in Surgery and Medicine},
   volume = {49},
   number = {4},
   pages = {361-365},
   ISSN = {1096-9101},
   DOI = {10.1002/lsm.22611},
   year = {2017},
   type = {Journal Article}
}

@article{Eibl:17,
author = {Matthias Eibl and Sebastian Karpf and Daniel Weng and Hubertus Hakert and Tom Pfeiffer and Jan Philip Kolb and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {Fiber optics imaging; Nonlinear optics, fibers; Lasers, fiber; Lifetime-based sensing; Fluorescence microscopy; Nonlinear microscopy; Fourier domain mode locking; Image quality; Imaging techniques; Laser sources; Pulsed fiber lasers; Three dimensional sensing},
number = {7},
pages = {3132--3142},
publisher = {Optica Publishing Group},
title = {Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate},
volume = {8},
month = {Jul},
year = {2017},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-8-7-3132},
doi = {10.1364/BOE.8.003132},
abstract = {Two-photon-excited fluorescence lifetime imaging microscopy (FLIM) is a chemically specific 3-D sensing modality providing valuable information about the microstructure, composition and function of a sample. However, a more widespread application of this technique is hindered by the need for a sophisticated ultra-short pulse laser source and by speed limitations of current FLIM detection systems. To overcome these limitations, we combined a robust sub-nanosecond fiber laser as the excitation source with high analog bandwidth detection. Due to the long pulse length in our configuration, more fluorescence photons are generated per pulse, which allows us to derive the lifetime with a single excitation pulse only. In this paper, we show high quality FLIM images acquired at a pixel rate of 1 MHz. This approach is a promising candidate for an easy-to-use and benchtop FLIM system to make this technique available to a wider research community.},
}

@article{Brinkmann2017,
   author = {Tode, J;Richert, E;von der Burchard, C;Koinzer, S;Klettner, A;Brinkmann, R and Roider, J},
   title = {Schonende retinale Lasertherapien als Behandlungsoption der trockenen AMD },
   journal = {Spitzenforschung in der Ophthalmologie},
   pages = {170-173},
   ISSN = {1861-4620},
   url = {https://www.dog.org/wp-content/uploads/2009/12/DOG_Sonderband_WEB-min.pdf#page=1&zoom=auto,-57,877},
   year = {2017},
   type = {Journal Article}
}

@article{Buj2017,
   author = {Buj, C; Münter, M; Schmarbeck, B; Horstmann, J; Hüttmann, G and Brinkmann, R},
   title = {Noncontact holographic detection for photoacoustic tomography},
   journal = {J Biomed Opt},
   
   pages = {1-14},
   DOI = {10.1117/1.jbo.22.10.106007},
   year = {2017},
   type = {Journal Article}
}

@article{Horstmann2017,
title = {Label-Free In Vivo Imaging of Corneal Lymphatic Vessels Using Microscopic Optical Coherence Tomography},
author = {Horstmann, J; Schulz-Hildebrandt, H; Bock, F; Siebelmann, S; Lankenau, E; H\"{u}ttmann, G; Steven, P and  Cursiefen, C},
editor = {The Association Research for in Vision and Ophthalmology},
doi = {10.1167/iovs.17-22286},
isbn = {1552-5783},
year = {2017},
date = {2017-12-25},
journal = { Investig Ophthal & Vis Scie},

pages = {5880-5886},
abstract = {Purpose: Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study investigates whether corneal lymphatic vessels can be imaged using microscopic optical coherence tomography (mOCT). 

Methods: Corneal neovascularization was induced by intrastromal placement of 11.0 nylon sutures in one eye of BALB/c mice. After 2 weeks, cross-sectional images and volumes of the corneas with a 0.5 mm lateral and axial field of view were acquired using a custom-built mOCT system enabling a resolution of 1 μm at a B-scan rate of 165/s. Three of the six animals received an additional intrastromal injection of India ink 24 hours before the measurement to stain the corneal lymphatic system in vivo. Immunohistochemistry using CD31 and LYVE-1 was used to validate the mOCT findings. 

Results: Using mOCT, lymphatic vessels were visible as dark vessel-like structures with the lumen lacking a hyperreflective wall and mostly lacking cells. However, individual, slowly moving particles, which most likely are immune cells, occasionally could be observed inside the lumen. In lymphatic vessels of ink-stained corneas, hyperreflection and shadowing underneath was observed. Ink-filled lymphatic vessels were colocalized in consecutive corneal flat mounts of the same specimen. 

Conclusions: Corneal lymphatic vessels can be imaged using mOCT. This novel approach opens new options for noninvasive clinical imaging of corneal lymphatic vessels for diagnostic and therapeutic indications.},
keywords = {OCM},
pubstate = {published},
tppubtype = {article}
}

@article{Yao2017,
   author = {Yao, C; Rudnitzki, F; Hüttmann, G; Zhang, Zand Rahmanzadeh, R},
   title = {Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation},
journal = {International Journal of Nanomedicine},
  
   pages = {5659-5672},
   DOI = {10.2147/IJN.S140620},
   year = {2017},
   type = {Journal Article}
}

  @article{Jirauschek2017,
   author = {Jirauschek, Christian and Huber, Robert},
   title = {Efficient simulation of the swept-waveform polarization dynamics in fiber spools and Fourier domain mode-locked (FDML) lasers},
   journal = {Journal of the Optical Society of America B},
   volume = {34},
   number = {6},
   pages = {1135-1146},
   DOI = {10.1364/JOSAB.34.001135},
   url = {http://josab.osa.org/abstract.cfm?URI=josab-34-6-1135},
   year = {2017},
   type = {Journal Article}
}

   abstract = {We present a theoretical model and its efficient numerical implementation for the simulation of wavelength-swept waveform propagation in fiber systems such as Fourier domain mode-locked (FDML) lasers, fully accounting for the polarization dynamics in fiber spools and further polarization-dependent optical components in the setup. This approach enables us to perform long-time simulations of the FDML laser dynamics over more than 100,000 cavity round trips, as required for some FDML configurations to ensure convergence to the steady-state operating regime. The model is validated against experimental results for single propagation through a fiber spool and for stationary FDML operation. The polarization dynamics due to the fiber spool, inducing polarization-mode dispersion, bending birefringence as well as cross-phase modulation, and other optical components such as the Faraday-rotating mirror used for polarization compensation is thoroughly investigated.},
   keywords = {Laser theory
Lasers, tunable
Optical coherence tomography
Birefringence
Polarization
Pulses
AG-Huber_FDML},
   url = {https://www.osapublishing.org/josab/abstract.cfm?uri=josab-34-6-1135},
   year = {2017},
   type = {Journal Article}
}

@article{Miura2017,
   author = {Miura, Y; Pruessner, J; Mertineit, C L; Kern, K; Muenter, M; Moltmann, M; Danicke, V and Brinkmann, R},
   title = {Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects},
   journal = {J Vis Exp},
   
   ISSN = {1940-087x},
   DOI = {10.3791/54326},
   year = {2017},
   type = {Journal Article}
  } 

@article{Miura2017,
   author = {Rudolf, M; Mir Mohi Sefat, A; Miura, Y; Tura, A; Raasch, W; Ranjbar, M; Grisanti, S; Aherrahrou, Z; Wagner, A; Messinger, J D; Garber, D W; Anantharamaiah, G M and Curcio, C A},
   title = {ApoA-I Mimetic Peptide 4F Reduces Age-Related Lipid Deposition in Murine Bruch's Membrane and Causes Its Structural Remodeling},
   journal = {Curr Eye Res},
   pages = {1-12},
   ISSN = {0271-3683},
   DOI = {10.1080/02713683.2017.1370118},
   year = {2017},
   type = {Journal Article},

}

@article{Verbytskyi2017,
   author = {Verbytskyi, Ievgen and Münter, Michael and Buj, Christian and Brinkmann, Ralf},
   title = {A Problem of a Displacement Calculation of Tissue Surface in Non-Contact Photoacoustic Tomography},
   journal = {Naukovi Visti NTUU KPI},
   number = {2},
   pages = {58-64},
   ISSN = {2519-8890},
   url = {http://dx.doi.org/10.20535/1810-0546.2017.2.98021},
   year = {2017},
   type = {Journal Article}
}

@article{Seiler2017,
   author = {Seiler, T G; Engler, M; Beck, E; Birngruber, R and Kochevar, I E},
   title = {Interface bonding with corneal crosslinking (CXL) after LASIK ex vivo},
   journal = {Investig Ophthal and Vis Scie},
   
   pages = {6292-6298},
   DOI = {10.1167/iovs.17-22426},
   
   year = {2017},
   type = {Journal Article}
}

@article{Horstmann2016,
   author = {Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.},
   title = {OCT verstehen – Teil 2: Praktische Aspekte und Anwendung},
   journal = {Augenheilkunde up2date},
   volume = {6},
   number = {04},
   pages = {305-320},
   ISSN = {1616-9719},
   DOI = {10.1055/s-0042-117459},
   year = {2016},
   type = {Journal Article}
}

@article{RN4897,
   author = {Sudkamp, Helge and Koch, Peter and Spahr, Hendrik and Hillmann, Dierck and Franke, Gesa and Münst, Michael and Reinholz, Fred and Birngruber, Reginald and Hüttmann, Gereon},
   title = {In-vivo retinal imaging with off-axis full-field time-domain optical coherence tomography},
   journal = {Optics Letters},
   volume = {41},
   number = {21},
   pages = {4987-4990},
   DOI = {10.1364/OL.41.004987},
   url = {http://ol.osa.org/abstract.cfm?URI=ol-41-21-4987},
   year = {2016},
   type = {Journal Article}
}

@article{Spahr2016,
   author = {Spahr, H. and Hillmann, D. and Hain, C. and Pfäffle, C. and Sudkamp, H. and Franke, G. and Koch, P. and Hüttmann, G.},
   title = {Darstellung von Blutfluss und Pulsation in retinalen Gefäßen mit Full-Field-Swept-Source-OCT},
   journal = {Klin Monatsbl Augenheilkd},
   volume = {233},
   number = {12},
   pages = {1324-1330},
   ISSN = {0023-2165},
   DOI = {10.1055/s-0042-120279},
   year = {2016},
   type = {Journal Article}
}

@article{Schulz-Hildebrandt2016,
   author = {Schulz-Hildebrandt, H. and Pieper, M. and Kasper, J. and Traulsen, N. and Mall, M. and König, P. and Hüttmann, G.},
   title = {Towards automated evaluation of mucus transport measured by microscopic OCT (mOCT) during hypertonic saline treatment of Cystic Fibrosis},
   journal = {Pneumologie},
   volume = {70},
   number = {07},
   pages = {1-48},
   ISSN = {0934-8387},
   DOI = {10.1055/s-0036-1584651},
   year = {2016},
   type = {Journal Article}
}

@article{Karpf:16,
author = {Sebastian Karpf and Matthias Eibl and Benjamin Sauer and Fred Reinholz and Gereon H\"{u}ttmann and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {Fiber optics imaging; Nonlinear optics, fibers; Lasers, fiber; Fluorescence microscopy; Nonlinear microscopy; Femtosecond pulses; In vivo imaging; Laser sources; Nanosecond pulses; Optical systems; Ultrafast lasers},
number = {7},
pages = {2432--2440},
publisher = {Optica Publishing Group},
title = {Two-photon microscopy using fiber-based nanosecond excitation},
volume = {7},
month = {Jul},
year = {2016},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-7-7-2432},
doi = {10.1364/BOE.7.002432},
abstract = {Two-photon excitation fluorescence (TPEF) microscopy is a powerful technique for sensitive tissue imaging at depths of up to 1000 micrometers. However, due to the shallow penetration, for in vivo imaging of internal organs in patients beam delivery by an endoscope is crucial. Until today, this is hindered by linear and non-linear pulse broadening of the femtosecond pulses in the optical fibers of the endoscopes. Here we present an endoscope-ready, fiber-based TPEF microscope, using nanosecond pulses at low repetition rates instead of femtosecond pulses. These nanosecond pulses lack most of the problems connected with femtosecond pulses but are equally suited for TPEF imaging. We derive and demonstrate that at given cw-power the TPEF signal only depends on the duty cycle of the laser source. Due to the higher pulse energy at the same peak power we can also demonstrate single shot two-photon fluorescence lifetime measurements.},
}

@article{doi:10.1021/acs.jpcc.6b02012,
author = {Huber, Robert and Dworak, Lars and Moser, Jacques E. and Grätzel, Michael and Wachtveitl, Josef},
title = {Beyond Vibrationally Mediated Electron Transfer: Coherent Phenomena Induced by Ultrafast Charge Separation},
journal = {The Journal of Physical Chemistry C},
volume = {120},
number = {16},
pages = {8534-8539},
year = {2016},
doi = {10.1021/acs.jpcc.6b02012},

URL = { 
        https://doi.org/10.1021/acs.jpcc.6b02012
    
},
eprint = { 
        https://doi.org/10.1021/acs.jpcc.6b02012
    
}
,
    abstract = { Wave packet propagation succeeding electron transfer (ET) from alizarin dye molecules into the nanocrystalline TiO2 semiconductor has been studied by ultrafast transient absorption spectroscopy. Because of the ultrafast time scale of the ET reaction of about 6 fs, the system shows substantial differences to molecular ET systems. We show that the ET process is not mediated by molecular vibrations, and therefore classical ET theories lose their applicability. Here the ET reaction itself prepares a vibrational wave packet and not the electromagnetic excitation by the laser pulse. Furthermore, the generation of phonons during polaron formation in the TiO2 lattice is observed in real time for this system. The presented investigations enable an unambiguous assignment of the involved photoinduced mechanisms and can contribute to a corresponding extension of molecular ET theories to ultrafast ET systems like alizarin/TiO2. }
}

@article{Hüttmann2016,
   author = {Huttmann, Gereon and Moltmann, Moritz and Spahr, Hendrik and Tode, Jan and de Roeck, Anna and Theisen-Kunde, Dirk and Birngruber, Reginald and Koinzer, Stefan and Brinkmann, Ralf},
   title = {Retinal lesion formation during photocoagulation investigated by high-speed 1060 nm Doppler-OCT: first clinical results},
   journal = {Investigative Ophthalmology & Visual Science},
   volume = {57},
   number = {12},
   pages = {5852-5852},
   abstract = {Abstract Purpose : The molecular processes during heating with a photocoagulation laser, particularly in sub-visible or mere thermal stimulation treatment, have only partly been understood, and different theories exist that try to explain its clinical efficacy. Optical coherence tomography (OCT) was successfully used to grade lesions with high accuracy 1 hour after the treatments and beyond. During the irradiation, changes in tissue scattering and, by use of the Doppler signal, tissue motion caused by thermal expansion and coagulation-induced tissue contraction were shown to correlate ex-vivo and in rabbits with the strength of photocoagulation lesions. Aim of this study was to validate feasibility and reproducibility of these results in humans. Methods : In an ongoing study more than 100 lesions of three patients have been imaged with a slitlamp-based OCT (1060 nm, 90,000 A-scans/s) with varying irradiance during laser exposure. Durations of the exposure were 50 ms and 200 ms; spot size was 300 µm. Eye movements and heart beat were corrected by cross-correlation of the images. Increased tissue scattering and movement of the neuronal retina due to thermal expansion were determined from the image sequences with 3 ms temporal resolution. Results : In the first treatments with this prototype device, we received acceptable image quality in 1/3 of the lesions. Changes in the neuronal retina were successful visualized during and after the laser irradiation, demonstrating the feasibility of a real-time assessment of initial effects of photocoagulation in humans. Lesion visibility in standard, reflection-based OCT was much weaker during treatment compared to 1 hour afterwards. Increased tissue scattering was observed in stronger lesions already during the laser irradiation. At reduced irradiance, scattering increase was only observed after the end of irradiation. However, tissue motion towards the vitreous was still observed in these cases. Conclusions : In conclusion, high-speed OCT recording during photocoagulation measures initial tissue changes during photocoagulation in humans. It may enhance our understanding of the tissue dynamics right after laser irradiation. It may provide useful information for a real-time dosage control as well. This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.},
   ISSN = {1552-5783},
   url = {http://dx.doi.org/},
   year = {2016},
   type = {Journal Article}
}

@article{Kim2016,
   author = {Kim, H. D. and Jang, S. Y. and Lee, S. H. and Kim, Y. S. and Ohn, Y. H. and Brinkmann, R. and Park, T. K.},
   title = {Retinal Pigment Epithelium Responses to Selective Retina Therapy in Mouse Eyes},
   journal = {Invest Ophthalmol Vis Sci},
   volume = {57},
   number = {7},
   pages = {3486-95},
   abstract = {PURPOSE: To investigate the characteristics of retinal pigment epithelium (RPE) and retinal damage induced by selective retina therapy (SRT) in mice, and to elucidate longitudinal changes in RPE cells. METHODS: C57BL/6J mice received SRT and continuous-wave laser photocoagulation (cwPC). The cell death pattern was evaluated using TUNEL assay, and proliferative potential of the RPE cells was evaluated using 5-ethynyl-2'-dexoyuridine (EdU) assay. To investigate the cell-cell integrity of RPE cells, beta-catenin staining was performed. The number and hexagonality of RPE cells in the SRT-treated area were estimated using a Voronoi diagram with time periods of 3 hours to 14 days. Antibodies to microphthalmia-associated transcription factor (MiTF) and orthodenticle homeobox 2 (Otx2) were used to confirm the specific characteristics of RPE cells in the SRT-treated area. RESULTS: The number of TUNEL-positive cells located in the neural retina was significantly lower in lesions treated with SRT compared to those treated with cwPC. EdU-positive RPE cells were first detected 3 to 12 hours after SRT, and increased until 3 to 7 days after SRT. beta-catenin staining showed that hexagonality was compromised and subsequently, RPE cells expanded in size within the targeted location. The number of RPE cells in SRT lesions decreased gradually until 12 hours after SRT and recovered by 14 days. Upregulated expression of MiTF and Otx2 was observed for 2 weeks in the SRT lesions. CONCLUSIONS: Selective retina therapy seems to induce selective RPE damage without collateral thermal injury in the neural retina. Furthermore, SRT-treated lesions recovered by proliferation of RPE cells that were present in the treated lesions and by expansion of adjacent RPE cells.},
   ISSN = {0146-0404},
   DOI = {10.1167/iovs.16-19508},
   year = {2016},
   type = {Journal Article}
}

@article{Linz2016,
   author = {Linz, Norbert and Freidank, Sebastian and Liang, Xiao-Xuan and Vogel, Alfred},
   title = {Wavelength dependence of femtosecond laser-induced breakdown in water and implications for laser surgery},
   journal = {American Physical Society,Phys. Rev. B},
   volume = { 94},
   number = {2},
   pages = {1-19},
   url = {http://link.aps.org/doi/10.1103/PhysRevB.94.024113},
   year = {2016},
   type = {Journal Article}
}

@article{Yasui2016,
   author = {Yasui, Ayako and Yamamoto, Manabu and Hirayama, Kumiko and Shiraki, Kunihiko and Theisen-Kunde, Dirk and Brinkmann, Ralf and Miura, Yoko and Kohno, Takeya},
   title = {Retinal sensitivity after selective retina therapy (SRT) on patients with central serous chorioretinopathy},
   journal = {Graefe's Archive for Clinical and Experimental Ophthalmology},
   pages = {1-12},
   abstract = {To assess retinal sensitivity after selective retina therapy (SRT) in patients with central serous chorioretinopathy (CSCR).},
   ISSN = {1435-702X},
   url = {http://dx.doi.org/10.1007/s00417-016-3441-8},
   year = {2016},
   type = {Journal Article}
}

@article{Park2016,
   author = {Park, Y. G. and Kim, J. R. and Kang, S. and Seifert, E. and Theisen-Kunde, D. and Brinkmann, R. and Roh, Y. J.},
   title = {Safety and efficacy of selective retina therapy (SRT) for the treatment of diabetic macular edema in Korean patients},
   journal = {Graefes Arch Clin Exp Ophthalmol},
   note = {1435-702x
Park, Young Gun
Kim, Jae Ryun
Kang, Seungbum
Seifert, Eric
Theisen-Kunde, Dirk
Brinkmann, Ralf
Roh, Young-Jung
Journal article
Graefes Arch Clin Exp Ophthalmol. 2016 Jan 23.},
   abstract = {PURPOSE: Selective retina therapy (SRT) stimulates retinal pigment epithelium (RPE) cell migration and proliferation into irradiated areas. The objective of this study was to evaluate the efficacy and safety of SRT in Korean patients with clinically significant diabetic macular edema (DME). METHODS: Prospective non-randomized interventional case series study. Twenty-three eyes of 21 patients with clinically significant DME were treated with SRT and followed for 6 months. Patients underwent an evaluation of best corrected visual acuity (BCVA) in Early Treatment Diabetic Retinopathy Study (ETDRS) letters. Microperimetry was employed to measure macular sensitivity within the central 10 degrees field, and the central macular thickness (CMT) and maximum macular thickness (MMT) were measured. RESULTS: An improvement in BCVA of one to two ETDRS lines was observed in 41.2 % of patients and an improvement of greater than two lines in 29.4 %. Although there was no significant change in CMT (P > 0.05), MMT decreased from 465.8 +/- 87.4 mum to 434.3 +/- 83.9 mum (P = 0.006), and mean macular sensitivity increased from 20.8 +/- 3.4dB to 22.5 +/- 3.5dB (P = 0.02). CONCLUSIONS: The gains in BCVA and improvement in macular sensitivity demonstrated that SRT may be used as an effective and safe treatment modality in Korean patients with clinically significant DME.},
   keywords = {Diabetic macular edema
Dosimetry
Microperimetry
Retinal pigment epithelium
Selective retina therapy},
   ISSN = {0721-832x},
   DOI = {10.1007/s00417-015-3262-1},
   year = {2016},
   type = {Journal Article}
}

@article{Ranjbar2016,
   author = {Ranjbar, Mahdy and Brinkmann, Max Philipp and Tura, Aysegül and Rudolf, Martin and Miura, Yoko and Grisanti, Salvatore},
   title = {Ranibizumab interacts with the VEGF-A/VEGFR-2 signaling pathway in human RPE cells at different levels},
   journal = {Cytokine},
   volume = {83},
   pages = {210-216},
   abstract = {Vascular endothelial growth factor (VEGF) secreted by the retinal pigment epithelium (RPE) plays an important role in ocular homeostasis, but also in diseases, most notably age-related macular degeneration (AMD). To date, anti-VEGF drugs like ranibizumab have been shown to be most effective in treating these pathologic conditions. However, clinical trials suggest that the RPE could degenerate and perish through anti-VEGF treatment. Herein, we evaluated possible pathways and outcomes of the interaction between ranibizumab and human RPE cells (ARPE-19). Results indicate that ranibizumab affects the VEGF-A metabolism in RPE cells from an extra- as well as intracellular site. The drug is taken up into the cells, with the VEGF receptor 2 (VEGFR-2) being involved, and decreases VEGF-A protein levels within the cells as well as extracellularly. Oxidative stress plays a key role in various inflammatory disorders of the eye. Our results suggest that oxidative stress inhibits RPE cell proliferation. This anti-proliferative effect on RPE cells is significantly enhanced through ranibizumab, which does not inhibit RPE cell proliferation substantially in absence of relevant oxidative stress. Therefore, we emphasize that anti-VEGF treatment should be selected carefully in AMD patients with preexistent extensive RPE atrophy.},
   keywords = {Ranibizumab
RPE
VEGF-A
VEGFR-2
Oxidative stress},
   ISSN = {1043-4666},
   url = {http://www.sciencedirect.com/science/article/pii/S1043466616300722},
   year = {2016},
   type = {Journal Article}
}

@article{Kang1016,
   author = {Kang, S. and Park, Y. G. and Kim, J. R. and Seifert, E. and Theisen-Kunde, D and Brinkman, R and Roh, Y. J.},
   title = {Selective Retina Therapy in Patients With Chronic Central Serous Chorioretinopathy: A Pilot Study},
   journal = {Medicine (Baltimore)},
   volume = {95},
   number = {3},
   pages = {e2524},
   note = {1536-5964
Kang, Seungbum
Park, Young Gun
Kim, Jae Ryun
Seifert, Eric
Dirk, Theisen-Kunde
Ralf, Brinkmann
Roh, Young Jung
Journal Article
United States
Medicine (Baltimore). 2016 Jan;95(3):e2524. doi: 10.1097/MD.0000000000002524.},
   abstract = {We evaluated visual outcomes, changes of maximum macular thickness (MMT) and subretinal fluid (SRF), and safety in patients with chronic central serous chorioretinopathy (CSC) after treatment with selective retina therapy (SRT). Retrospective cohort study of patients with chronic CSC presenting to a university-based hospital from January 2014 through January 2015 was conducted. A total of 12 eyes of 12 patients with chronic CSC lasting for at least 3 months was recruited. The follow-up period ranged from 3 to 12 months. Following evaluation of test spots at temporal arcades, SRT (Q-switched neodymium-doped yttrium lithium fluoride [Nd:YLF] laser; wavelength, 527 nm, pulse duration, 1.7 microsececond) was applied to the surrounding areas of leakage observed on fluorescein angiogram and/or pigment epithelial detachment (PED). Changes in best-correct visual acuity (BCVA), MMT, and SRF and macular sensitivity (MS) by microperimetry (MP) were evaluated. Eyes received treatment in a mean of 3.83 spots at the pulse energy of 65 to 90 muJ. Mean BCVA (logMAR) improved from 0.23 +/- 0.12 at baseline to 0.14 +/- 0.13 at 3 months. MMT decreased from 341.4 +/- 85.5 mum at baseline to 236.0 +/- 57.9 mum at 3 months. SRF completely resolved in 75% (9 eyes) at 3 months. Large PEDs (2 eyes) were flattened at 3 months. Retreatment was performed in 4 eyes. MP showed no evidence of scotoma around SRT-treated lesions. SRT treatment targeting the surrounding area of leakage point showed favorable visual and structural outcomes in chronic CSC patients without the risk of scotoma.},
   ISSN = {0025-7974},
   DOI = {10.1097/md.0000000000002524},
   year = {2016},
   type = {Journal Article}
}

@article{Horstmann2016,
   author = {Horstmann, J. and Siebelmann, S. and Schulz-Hildebrandt, H. and Glasunow, I. and Schadschneider, A. and Hüttmann, G.},
   title = {OCT verstehen – Teil 1: Physikalische Grundlagen},
   journal = {Augenheilkunde up2date},
   volume = {6},
   number = {04},
   pages = {289-300},
   ISSN = {1616-9719},
   DOI = {10.1055/s-0042-113337},
   year = {2016},
   type = {Journal Article}
}

@article{Hillmann2016,
   author = {Hillmann, Dierck and Spahr, Hendrik and Hain, Carola and Sudkamp, Helge and Franke, Gesa and Pfäffle, Clara and Winter, Christian and Hüttmann, Gereon},
   title = {Aberration-free volumetric high-speed imaging of in vivo retina},
   journal = {Scientific Reports},
   volume = {6},
   pages = {1-11},
   url = {http://dx.doi.org/10.1038/srep35209},
   year = {2016},
   type = {Journal Article}
}

@article{Pieper2016,
   author = {Pieper, M. and Schulz-Hildebrandt, H. and Mall, M. and Hüttmann, G. and König, P.},
   title = {Intravital microscopy of mucus transport in mice provides mechanistic insight into hypertonic saline treatment of Cystic Fibrosis},
   journal = {Pneumologie},
   volume = {70},
   number = {07},
   pages = {SOP2},
   ISSN = {0934-8387},
   DOI = {10.1055/s-0036-1584654},
   year = {2016},
   type = {Journal Article}
}

@article{Wang2016,
   author = {Wang, Sijia and Hüttmann, Gereon and Rudnitzki, Florian and Diddens-Tschoeke, Heyke and Zhang, Zhenxi and Rahmanzadeh, Ramtin},
   title = {Indocyanine green as effective antibody conjugate for intracellular molecular targeted photodynamic therapy},
   journal = {Journal of Biomedical Optics},
   volume = {21},
   number = {7},
   pages = {078001-078001},
   note = {10.1117/1.JBO.21.7.078001},
   abstract = {Abstract.  The fluorescent dye indocyanine green (ICG) is clinically approved and has been applied for ophthalmic and intraoperative angiography, measurement of cardiac output and liver function, or as contrast agent in cancer surgery. Though ICG is known for its photochemical effects, it has played a minor role so far in photodynamic therapy or techniques for targeted protein-inactivation. Here, we investigated ICG as an antibody-conjugate for the selective inactivation of the protein Ki-67 in the nucleus of cells. Conjugates of the Ki-67 antibody TuBB-9 with different amounts of ICG were synthesized and delivered into HeLa and OVCAR-5 cells through conjugation to the nuclear localization sequence. Endosomal escape of the macromolecular antibodies into the cytoplasm was optically triggered by photochemical internalization with the photosensitizer BPD. The second light irradiation at 690 nm inactivated Ki-67 and subsequently caused cell death. Here, we show that ICG as an antibody-conjugate can be an effective photosensitizing agent. Best effects were achieved with 1.8 ICG molecules per antibody. Conjugated to antibodies, the ICG absorption peaks vary proportionally with concentration. The absorption of ICG above 650 nm within the optical window of tissue opens the possibility of selective Ki-67 inactivation deep inside of tissues.},
   ISSN = {1083-3668},
   year = {2016},
   type = {Journal Article}
}

@article{Hillmann2016,
   author = {Hillmann, Dierck and Spahr, Hendrik and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon},
   title = {In vivo optical imaging of physiological responses to photostimulation in human photoreceptors},
   journal = {PNAS Early Edition},
   pages = {1-6},
   abstract = {Noninvasive functional imaging of molecular and cellular processes of vision may have immense impact on research and clinical diagnostics. Although suitable intrinsic optical signals (IOSs) have been observed ex vivo and in immobilized animals in vivo, detecting IOSs of photoreceptor activity in living humans was cumbersome and time consuming. Here, we observed clear spatially and temporally resolved changes in the optical path length of the photoreceptor outer segment as a response to an optical stimulus in the living human eye. To witness these changes, we evaluated phase data obtained with a parallelized and computationally aberration-corrected optical coherence tomography system. The noninvasive detection of optical path length changes shows neuronal photoreceptor activity of single cones in living human retina, and therefore, it may provide diagnostic options in ophthalmology and neurology and could provide insights into visual phototransduction in humans.},
   url = {http://www.pnas.org/content/early/2016/10/10/1606428113.abstract},
   year = {2016},
   type = {Journal Article}
}

@article{WANG2016622,
title = {Heartbeat OCT and Motion-Free 3D In Vivo Coronary Artery Microscopy},
journal = {JACC: Cardiovascular Imaging},
volume = {9},
number = {5},
pages = {622-623},
year = {2016},
issn = {1936-878X},
doi = {https://doi.org/10.1016/j.jcmg.2015.08.010},
url = {https://www.sciencedirect.com/science/article/pii/S1936878X15006713},
author = {Tianshi Wang and Tom Pfeiffer and Evelyn Regar and Wolfgang Wieser and Heleen {van Beusekom} and Charles T. Lancee and Geert Springeling and Ilona Krabbendam-Peters and Antonius F.W. {van der Steen} and Robert Huber and Gijs {van Soest}}
}

@article{Wang2016,
   author = {Wang, S. and Huttmann, G. and Scholzen, T. and Zhang, Z. and Vogel, A. and Hasan, T. and Rahmanzadeh, R.},
   title = {A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67},
   journal = {Sci Rep},
   volume = {6},
   pages = {27032},
   note = {2045-2322
Wang, Sijia
Huttmann, Gereon
Scholzen, Thomas
Zhang, Zhenxi
Vogel, Alfred
Hasan, Tayyaba
Rahmanzadeh, Ramtin
Journal Article
England
Sci Rep. 2016 Jun 1;6:27032. doi: 10.1038/srep27032.},
   abstract = {Using nanotechnology for optical manipulation of molecular processes in cells with high spatial and temporal precision promises new therapeutic options. Especially tumor therapy may profit as it requires a combination of both selectivity and an effective cell killing mechanism. Here we show a dual targeting approach for selective and efficient light-controlled killing of cells which are positive for epidermal growth factor receptor (EGFR) and Ki-67. Liposomes with the covalently linked EGFR antibody Erbitux enabled selective uptake of FITC-labeled Ki-67 antibody TuBB-9 in EGFR-positive cells pre-loaded with the photoactive dye BPD. After irradiation at 690 nm, BPD disrupted the endosomal membranes and delivered the antibodies to the nucleoli of the cells. The second irradiation at 490 nm activated the FITC-labeled TuBB-9, which caused inactivation of the Ki-67 protein and subsequent cell death via apoptosis. Efficient cell killing was possible at nanomolar concentrations of TuBB-9 due to the effective transport by immune liposomes and the high efficacy of the Ki-67 light-inactivation. Delivery of the liposomal constructs and cell destruction correlated well with the EGFR expression pattern of different cell lines (HeLa, OVCAR-5, MCF-7, and human fibroblasts), demonstrating an excellent selectivity.},
   ISSN = {2045-2322},
   DOI = {10.1038/srep27032},
   year = {2016},
   type = {Journal Article}
}

@article{Ranjbar2016,
   author = {Ranjbar, M. and Brinkmann, M. P. and Zapf, D. and Miura, Y. and Rudolf, M. and Grisanti, S.},
   title = {Fc Receptor Inhibition Reduces Susceptibility to Oxidative Stress in Human RPE Cells Treated with Bevacizumab, but not Aflibercept},
   journal = {Cell Physiol Biochem},
   volume = {38},
   number = {2},
   pages = {737-47},
   note = {1421-9778
Ranjbar, Mahdy
Brinkmann, Max Philipp
Zapf, Dorinja
Miura, Yoko
Rudolf, Martin
Grisanti, Salvatore
Journal Article
Switzerland
Cell Physiol Biochem. 2016;38(2):737-47. doi: 10.1159/000443030. Epub 2016 Feb 15.},
   abstract = {BACKGROUND/AIMS: VEGF-A is induced by oxidative stress, and functions as a survival factor for various cell types, including retinal pigment epithelial (RPE) cells. Anti-vascular endothelial growth factor (VEGF) drugs like aflibercept and bevacizumab have shown to be most effective in treating neovascular age-related macular degeneration (AMD), however uptake of the drugs might lead to interference with cell physiology. Herein, we evaluated the significance of the Fc receptor (FcR) within this context and moreover explored the impact of VEGF inhibition under normal conditions as well as under oxidative stress, in terms of potential adverse effects. METHODS: ARPE-19 (human RPE) cells were treated with aflibercept and bevacizumab in presence or absence of H2O2 as oxidative stress stimulus. After 24h cells were evaluated for drug uptake, VEGF-A expression and secretion, levels of intracellular reactive oxygen species (ROS) as well as cell proliferation. Experiments were repeated with cells being pre-incubated with an FcR inhibitor prior to drug application. RESULTS: Both drugs inhibited extracellular levels of VEGF-A and were taken up into the RPE, resulting in significantly reduced intracellular levels of VEGF-A. When oxidative stress was applied, intracellular ROS levels in cells treated with both drugs rose, and cell proliferation was reduced. Prior incubation with the FcR inhibitor lessened the uptake of bevacizumab, but not aflibercept into RPE cells, and simultaneously enhanced cell survival under oxidative stress conditions. CONCLUSIONS: Our results indicate that uptake and accumulation of aflibercept and bevacizumab within RPE cells affect the intracellular VEGF-A metabolism negatively, leading to a biologically relevant reduced cell survival under oxidative stress. The FcR plays a substantial role in the uptake of bevacizumab, but not aflibercept, which allows an enhanced RPE cell survival through FcR blockage in an environment dominated by oxidative stress, as clinically significant for various inflammatory retinal disorders.},
   ISSN = {1015-8987},
   DOI = {10.1159/000443030},
   year = {2016},
   type = {Journal Article}
}

@article{Maushagen2016,
   title        = {Effects of paclitaxel on permanent head and neck squamous cell carcinoma cell lines and identification of anti-apoptotic caspase 9b},
   author       = {Maushagen, R. and Reers, S. and Pfannerstill, A. C. and Hahlbrock, A. and Stauber, R. and Rahmanzadeh, R. and Rades, D. and Pries, R. and Wollenberg, B.},
   year         = 2016,
   journal      = {J Cancer Res Clin Oncol},
   volume       = 142,
   number       = 6,
   pages        = {1261--71},
   doi          = {10.1007/s00432-016-2150-3},
   issn         = {0171-5216},
   note         = {1432-1335 Maushagen, Regina Reers, Stefan Pfannerstill, Ann-Christin Hahlbrock, Angelina Stauber, Roland Rahmanzadeh, Ramtin Rades, Dirk Pries, Ralph Wollenberg, Barbara Journal Article Germany J Cancer Res Clin Oncol. 2016 Jun;142(6):1261-71. doi: 10.1007/s00432-016-2150-3. Epub 2016 Apr 1.},
   abstract     = {PURPOSE: Paclitaxel is an effective chemotherapeutic agent against various human tumors inducing apoptosis via binding to beta-tubulin of microtubules and arresting cells mainly in the G2/M phase of the cell cycle. However, the underlying specific molecular mechanisms of paclitaxel on head and neck squamous cell carcinoma (HNSCC) have not been identified yet. METHODS: The apoptotic effects and mechanisms of paclitaxel on different permanent HPV-negative HNSCC cell lines (UT-SCC-24A, UT-SCC-24B, UT-SCC-60A and UT-SCC-60B) were determined by flow cytometry assays, polymerase chain reaction analysis, immunofluorescence-based assays and sequencing studies. RESULTS: Paclitaxel induced a G2/M arrest in HNSCC cell lines followed by an increased amount of apoptotic cells. Moreover, the activation of caspase 8, caspase 10 and caspase 3, and the loss of the mitochondrial outer membrane potential could be observed, whereas an activation of caspase 9 could barely be detected. The efficient activation of caspase 9 was not affected by altered methylation patterns. Our results can show that the promoter region of apoptotic protease activating factor 1 (Apaf-1) was not methylated in the HNSCC cell lines. By sequencing analysis two isoforms of caspase 9, the pro-apoptotic caspase 9 and the anti-apoptotic caspase 9b were identified. The anti-apoptotic caspase 9b is missing the catalytic site and acts as an endogenous inhibitor of apoptosis by blocking the binding of caspase 9 to Apaf-1 to form the apoptosome. CONCLUSION: Our data indicate the presence of anti-apoptotic caspase 9b in HNSCC, which may serve as a promising target to increase chemotherapeutic apoptosis induction.},
   keywords     = {Apoptosis Caspase 9b Caspases Head and neck cancer Paclitaxel},
   type         = {Journal Article}
}

@article{RN5040,
   author = {Tromberg, B. J. and Anderson, R. R. and Birngruber, R. and Brinkmann, R. and Berns, M. W. and Parrish, J. A. and Apiou-Sbirlea, G.},
   title = {Biomedical optics centers: forty years of multidisciplinary clinical translation for improving human health},
   journal = {J Biomed Opt},
   volume = {21},
   number = {12},
   pages = {124001},
   ISSN = {1560-2281 (Electronic)
1083-3668 (Linking)},
   DOI = {10.1117/1.JBO.21.12.124001},
  
   year = {2016},
   type = {Journal Article}
}

@article{Schlott2016,
   author = {Schlott, Kerstin and Koinzer, Stefan and Baade, Alexander and Birngruber, Reginald and Roider, Johann and Brinkmann, Ralf},
   title = {Lesion strength control by automatic temperature guided retinal photocoagulation},
   journal = {Journal of Biomedical Optics},
   volume = {21},
   number = {9},
   pages = {098001-098001},
   note = {10.1117/1.JBO.21.9.098001},
   abstract = {Abstract.  Laser photocoagulation is an established treatment for a variety of retinal diseases. However, when using the same irradiation parameter, the size and strength of the lesions are unpredictable due to unknown inter- and intraindividual optical properties of the fundus layers. The aim of this work is to investigate a feedback system to generate desired lesions of preselectable strengths by automatically controlling the irradiation time. Optoacoustics were used for retinal temperature monitoring. A 532-nm continuous wave Nd:YAG laser was used for photocoagulation. A 75-ns/523-nm Q-switched Nd:YLF laser simultaneously excited temperature-dependent pressure transients, which were detected at the cornea by an ultrasonic transducer embedded in a contact lens. The temperature data were analyzed during the irradiation by a LabVIEW routine. The treatment laser was switched off automatically when the required lesion strength was achieved. Five different feedback control algorithms for different lesion sizes were developed and tested on rabbits in vivo. With a laser spot diameter of 133  μm, five different lesion types with ophthalmoscopically visible diameters ranging mostly between 100 and 200  μm, and different appearances were achieved by automatic exposure time control. The automatically controlled lesions were widely independent of the treatment laser power and the retinal pigmentation.},
   ISSN = {1083-3668},
   DOI = {10.1117/1.JBO.21.9.098001},
   year = {2016},
   type = {Journal Article}
}

@article{Wang:15,
author = {Tianshi Wang and Tom Pfeiffer and Evelyn Regar and Wolfgang Wieser and Heleen van Beusekom and Charles T. Lancee and Geert Springeling and Ilona Krabbendam and Antonius F.W. van der Steen and Robert Huber and Gijs van Soest},
journal = {Biomed. Opt. Express},
keywords = {Fiber optics imaging; Three-dimensional image acquisition; Medical optics instrumentation; Scanners; Endoscopic imaging; Medical and biological imaging; Optical coherence tomography; Image quality; Image registration; Imaging techniques; Laser modes; Mode locking; Optical coherence tomography},
number = {12},
pages = {5021--5032},
publisher = {Optica Publishing Group},
title = {Heartbeat OCT: in vivo intravascular megahertz-optical coherence tomography},
volume = {6},
month = {Dec},
year = {2015},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-6-12-5021},
doi = {10.1364/BOE.6.005021},
abstract = {Cardiac motion artifacts, non-uniform rotational distortion and undersampling affect the image quality and the diagnostic impact of intravascular optical coherence tomography (IV-OCT). In this study we demonstrate how these limitations of IV-OCT can be addressed by using an imaging system that we called \&\#x201C;Heartbeat OCT\&\#x201D;, combining a fast Fourier Domain Mode Locked laser, fast pullback, and a micromotor actuated catheter, designed to examine a coronary vessel in less than one cardiac cycle. We acquired in vivo data sets of two coronary arteries in a porcine heart with both Heartbeat OCT, working at 2.88 MHz A-line rate, 4000 frames/s and 100 mm/s pullback speed, and with a commercial system. The in vivo results show that Heartbeat OCT provides faithfully rendered, motion-artifact free, fully sampled vessel wall architecture, unlike the conventional IV-OCT data. We present the Heartbeat OCT system in full technical detail and discuss the steps needed for clinical translation of the technology.},
}

@article{Spahr2015,
   author = {Spahr, Hendrik and Hillmann, Dierck and Hain, Carola and Pfäffle, Clara and Sudkamp, Helge and Franke, Gesa and Hüttmann, Gereon},
   title = {Imaging pulse wave propagation in human retinal vessels using full-field swept-source optical coherence tomography},
   journal = {Optics Letters},
   volume = {40},
   number = {20},
   pages = {4771-4774},
   abstract = {We demonstrate a new noninvasive method to assess biomechanical properties of the retinal vascular system. Phase-sensitive full-field swept-source optical coherence tomography (PhS-FF-SS-OCT) is used to investigate retinal vascular dynamics at unprecedented temporal resolution. The motion of retinal tissue that is induced by expansion of the vessels therein is measured with an accuracy of about 10 nm. The pulse shapes of arterial and venous pulsations, their temporal delays, as well as the frequency-dependent pulse propagation through the capillary bed, are determined. For the first time, imaging speed and motion sensitivity are sufficient for a direct measurement of pulse waves propagating with more than 600 mm/s in retinal vessels of a healthy young subject.},
   keywords = {Optical coherence tomography
Ophthalmology
Time-resolved imaging
Functional monitoring and imaging},
   DOI = {10.1364/OL.40.004771},
   url = {http://ol.osa.org/abstract.cfm?URI=ol-40-20-4771},
   year = {2015},
   type = {Journal Article}
}

@article{10.1167/iovs.15-16670,
    author = {Mohler, Kathrin J. and Draxinger, Wolfgang and Klein, Thomas and Kolb, Jan Philip and Wieser, Wolfgang and Haritoglou, Christos and Kampik, Anselm and Fujimoto, James G. and Neubauer, Aljoscha S. and Huber, Robert and Wolf, Armin},
    title = "{Combined 60° Wide-Field Choroidal Thickness Maps and High-Definition En Face Vasculature Visualization Using Swept-Source Megahertz OCT at 1050 nm}",
    journal = {Investigative Ophthalmology & Visual Science},
    volume = {56},
    number = {11},
    pages = {6284-6293},
    year = {2015},
    month = {10},
    abstract = "{   To demonstrate ultrahigh-speed swept-source optical coherence tomography (SS-OCT) at 1.68 million A-scans/s for choroidal imaging in normal and diseased eyes over a ∼60° field of view. To investigate and correlate wide-field three-dimensional (3D) choroidal thickness (ChT) and vascular patterns using ChT maps and coregistered high-definition en face images extracted from a single densely sampled Megahertz-OCT (MHz-OCT) dataset.    High-definition, ∼60° wide-field 3D datasets consisting of 2088 × 1024 A-scans were acquired using a 1.68 MHz prototype SS-OCT system at 1050 nm based on a Fourier-domain mode-locked laser. Nine subjects (nine eyes) with various chorioretinal diseases or without ocular pathology are presented. Coregistered ChT maps, choroidal summation maps, and depth-resolved en face images referenced to either the retinal pigment epithelium or the choroidal–scleral interface were generated using manual segmentation.    Wide-field ChT maps showed a large inter- and intraindividual variance in peripheral and central ChT. In only four of the nine eyes, the location with the largest ChT was coincident with the fovea. The anatomy of the large lumen vessels of the outer choroid seems to play a major role in determining the global ChT pattern. Focal ChT changes with large thickness gradients were observed in some eyes.    Different ChT and vascular patterns could be visualized over ∼60° in patients for the first time using OCT. Due to focal ChT changes, a high density of thickness measurements may be favorable. High-definition depth-resolved en face images are complementary to cross sections and thickness maps and enhance the interpretation of different ChT patterns.  }",
    issn = {1552-5783},
    doi = {10.1167/iovs.15-16670},
    url = {https://doi.org/10.1167/iovs.15-16670},
    eprint = {https://arvojournals.org/arvo/content\_public/journal/iovs/934564/i1552-5783-56-11-6284.pdf},
}

@article{Diddens-Tschoeke2015,
   author = {Diddens-Tschoeke, Heyke C. and Hüttmann, Gereon and Gruber, Achim D. and Pottier, Roy H. and Hanken, Henning},
   title = {Localized thermal tumor destruction using dye-enhanced photothermal tumor therapy},
   journal = {Lasers in Surgery and Medicine},
   pages = {n/a-n/a},
   keywords = {photothermal therapy
naphthalocyanine derivative
in-vivo
laser
dye-enhanced},
   ISSN = {1096-9101},
   DOI = {10.1002/lsm.22356},
   url = {http://dx.doi.org/10.1002/lsm.22356},
   year = {2015},
   type = {Journal Article}
}

@article{Reznicek2015,
   author = {Reznicek, Lukas and Kolb, Jan P. and Klein, Thomas and Mohler, Kathrin J. and Wieser, Wolfgang and Huber, Robert and Kernt, Marcus and Märtz, Josef and Neubauer, Aljoscha S.},
   title = {Wide-Field Megahertz OCT Imaging of Patients with Diabetic Retinopathy},
   journal = {Journal of Diabetes Research},
   volume = {2015, Article ID 305084},
   pages = {1-5},
   DOI = {10.1155/2015/305084},
   url = {http://dx.doi.org/10.1155/2015/305084},
   year = {2015},
keywords = {AG-Huber_OCT},
   type = {Journal Article}

}

@article{Schulz-Hildebrandt2015,
   author = {Schulz-Hildebrandt, H. and Pieper, M. and König, P. and Hüttmann, G.},
   title = {Improved endoscopic optical coherence microscopy for imaging of humans airways in patients},
   journal = {Pneumologie},
   volume = {69},
   number = {07},
   pages = {A49},
   ISSN = {0934-8387},
   DOI = {10.1055/s-0035-1556641},
   year = {2015},
   type = {Journal Article}
}

@article{Jirauschek:15,
author = {Christian Jirauschek and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {Laser theory; Lasers, tunable; Optical coherence tomography; Vertical cavity surface emitting lasers; Distributed Bragg reflectors; Laser light; Laser sources; Quantum well lasers; Swept lasers; Vertical cavity surface emitting lasers},
number = {7},
pages = {2448--2465},
publisher = {Optica Publishing Group},
title = {Wavelength shifting of intra-cavity photons: Adiabatic wavelength tuning in rapidly wavelength-swept lasers},
volume = {6},
month = {Jul},
year = {2015},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-6-7-2448},
doi = {10.1364/BOE.6.002448},
abstract = {We analyze the physics behind the newest generation of rapidly wavelength tunable sources for optical coherence tomography (OCT), retaining a single longitudinal cavity mode during operation without repeated build up of lasing. In this context, we theoretically investigate the currently existing concepts of rapidly wavelength-swept lasers based on tuning of the cavity length or refractive index, leading to an altered optical path length inside the resonator. Specifically, we consider vertical-cavity surface-emitting lasers (VCSELs) with microelectromechanical system (MEMS) mirrors as well as Fourier domain mode-locked (FDML) and Vernier-tuned distributed Bragg reflector (VT-DBR) lasers. Based on heuristic arguments and exact analytical solutions of Maxwell's equations for a fundamental laser resonator model, we show that adiabatic wavelength tuning is achieved, i.e., hopping between cavity modes associated with a repeated build up of lasing is avoided, and the photon number is conserved. As a consequence, no fundamental limit exists for the wavelength tuning speed, in principle enabling wide-range wavelength sweeps at arbitrary tuning speeds with narrow instantaneous linewidth.},
}

@article{Huber:15,
author = {Robert Huber},
journal = {Opt. Photon. News},
keywords = {Image processing; Optical coherence tomography; Lasers, tunable; Medical optics and biotechnology; Optical coherence tomography; Image processing; Imaging techniques; Line scan cameras; Medical imaging; Optical coherence tomography; Three dimensional imaging},
number = {6},
pages = {32--39},
publisher = {Optica Publishing Group},
title = {4-D Real-Time Optical Coherence Tomography},
volume = {26},
month = {Jun},
year = {2015},
url = {https://www.optica-opn.org/abstract.cfm?URI=opn-26-6-32},
doi = {10.1364/OPN.26.6.000032},
abstract = {Advances in OCT techniques, combined with the processing power of moderncomputer hardware, are adding a new dimension---time---to a familiar 3-D imaging method.The result could be new applications in research and the biomedicalclinic.},
}

@article{Kolb:15,
author = {Jan Philip Kolb and Thomas Klein and Corinna L. Kufner and Wolfgang Wieser and Aljoscha S. Neubauer and Robert Huber},
journal = {Biomed. Opt. Express},
keywords = {Medical optics instrumentation; Lasers, fiber; Medical and biological imaging; Ophthalmic optics and devices ; Optical coherence tomography; Adaptive optics; Full field optical coherence tomography; Image quality; Imaging techniques; Laser scanning; Three dimensional imaging},
number = {5},
pages = {1534--1552},
publisher = {Optica Publishing Group},
title = {Ultra-widefield retinal MHz-OCT imaging with up to 100 degrees viewing angle},
volume = {6},
month = {May},
year = {2015},
url = {https://opg.optica.org/boe/abstract.cfm?URI=boe-6-5-1534},
doi = {10.1364/BOE.6.001534},
abstract = {We evaluate strategies to maximize the field of view (FOV) of in vivo retinal OCT imaging of human eyes. Three imaging modes are tested: Single volume imaging with 85{\textdegree} FOV as well as with 100{\textdegree} and stitching of five 60{\textdegree} images to a 100{\textdegree} mosaic (measured from the nodal point). We employ a MHz-OCT system based on a 1060nm Fourier domain mode locked (FDML) laser with a depth scan rate of 1.68MHz. The high speed is essential for dense isotropic sampling of the large areas. Challenges caused by the wide FOV are discussed and solutions to most issues are presented. Detailed information on the design and characterization of our sample arm optics is given. We investigate the origin of an angle dependent signal fall-off which we observe towards larger imaging angles. It is present in our 85{\textdegree} and 100{\textdegree} single volume images, but not in the mosaic. Our results suggest that 100{\textdegree} FOV OCT is possible with current swept source OCT technology.},
}

@article{Jirauschek:15,
author = {Christian Jirauschek and Robert Huber},
journal = {Opt. Lett.},
keywords = {Laser theory; Lasers, tunable; Optical coherence tomography; Birefringence; Polarization; Pulses; Cross phase modulation; Mode locking; Optical components; Picosecond pulses; Polarization mode dispersion; Pulse generation},
number = {10},
pages = {2385--2388},
publisher = {Optica Publishing Group},
title = {Modeling and analysis of polarization effects in Fourier domain mode-locked lasers},
volume = {40},
month = {May},
year = {2015},
url = {https://opg.optica.org/ol/abstract.cfm?URI=ol-40-10-2385},
doi = {10.1364/OL.40.002385},
abstract = {We develop a theoretical model for Fourier domain mode-locked (FDML) lasers in a non-polarization-maintaining configuration, which is the most widely used type of FDML source. This theoretical approach is applied to analyze a widely wavelength-swept FDML setup, as used for picosecond pulse generation by temporal compression of the sweeps. We demonstrate that good agreement between simulation and experiment can only be obtained by including polarization effects due to fiber bending birefringence, polarization mode dispersion, and cross-phase modulation into the theoretical model. Notably, the polarization dynamics are shown to have a beneficial effect on the instantaneous linewidth, resulting in improved coherence and thus compressibility of the wavelength-swept FDML output.},
}

@Article{HU_2015_Karpf_a,
  Title                    = {A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy},
  Author                   = {Karpf, Sebastian and Eibl, Matthias and Wieser, Wolfgang and Klein, Thomas and Huber, Robert},
  Journal                  = {Nature Communications},
  Year                     = {2015},
  Volume = {6},
  pages = {6784 1--6},
keywords = {AG-Huber_NL},
  Doi                      = {10.1038/ncomms7784}
}

@article{Framme2015,
   author = {Framme, C. and Walter, A. and Berger, L. and Prahs, P. and Alt, C. and Theisen-Kunde, D. and Kowal, J. and Brinkmann, R.},
   title = {Selective Retina Therapy in Acute and Chronic-Recurrent Central Serous Chorioretinopathy},
   journal = {Ophthalmologica},
   volume = {234},
   number = {4},
   pages = {177-88},
   note = {1423-0267
Framme, Carsten
Walter, Andreas
Berger, Lieselotte
Prahs, Philipp
Alt, Clemens
Theisen-Kunde, Dirk
Kowal, Jens
Brinkmann, Ralf
Journal Article
Research Support, Non-U.S. Gov't
Switzerland
Ophthalmologica. 2015;234(4):177-88. doi: 10.1159/000439188. Epub 2015 Sep 15.},
   abstract = {PURPOSE: Selective retina therapy (SRT), the confined laser heating and destruction of retinal pigment epithelial cells, has been shown to treat acute types of central serous chorioretinopathy (CSC) successfully without damaging the photoreceptors and thus avoiding laser-induced scotoma. However, a benefit of laser treatment for chronic forms of CSC is questionable. In this study, the efficacy of SRT by means of the previously used 1.7-micros and shorter 300-ns pulse duration was evaluated for both types of CSC, also considering re-treatment for nonresponders. MATERIAL AND METHODS: In a two-center trial, 26 patients were treated with SRT for acute (n = 10) and chronic-recurrent CSC (n = 16). All patients presented with subretinal fluid (SRF) in OCT and leakage in fluorescein angiography (FA). SRT was performed using a prototype SRT laser system (frequency-doubled Q-switched Nd:YLF-laser, wavelength 527 nm) with adjustable pulse duration. The following irradiation settings were used: a train of 30 laser pulses with a repetition rate of 100 Hz and pulse durations of 300 ns and 1.7 micros, pulse energy 120-200 microJ, retinal spot size 200 microm. Because SRT lesions are invisible, FA was always performed 1 h after treatment to demonstrate laser outcome (5-8 single spots in the area of leakage). In cases where energy was too low, as indicated by missing FA leakage, energy was adjusted and the patient re-treated immediately. Observation intervals were after 4 weeks and 3 months. In case of nonimprovement of the disease after 3 months, re-treatment was considered. RESULTS: Of 10 patients with active CSC that presents focal leakage in FA, 5 had completely resolved fluid after 4 weeks and all 10 after 3 months. Mean visual acuity increased from 76.6 ETDRS letters to 85.0 ETDRS letters 3 months after SRT. Chronic-recurrent CSC was characterized by less severe SRF at baseline in OCT and weaker leakage in FA than in acute types. Visual acuity changed from baseline 71.6 to 72.8 ETDRS letters after 3 months. At this time, SRF was absent in 3 out of 16 patients (19%), FA leakage had come to a complete stop in 6 out of 16 patients (38%). In 6 of the remaining chronic CSC patients, repeated SRT with higher pulse energy was considered because of persistent leakage activity. After the re-treatment, SRF resolved completely in 5 patients (83.3%) after only 25 days. CONCLUSION: SRT showed promising results in treating acute CSC, but was less effective in chronic cases. Interestingly, re-treatment resulted in enhanced fluid resolution and dry conditions after a considerably shorter time in most patients. Therefore, SRT including re-treatment if necessary might be a valuable CSC treatment alternative even in chronic-recurrent cases.},
   ISSN = {0030-3755},
   DOI = {10.1159/000439188},
   year = {2015},
   type = {Journal Article}
}

@article{Linz2015,
   author = {Linz, Norbert and Freidank, Sebastian and Liang, Xiao-Xuan and Vogelmann, Hannes and Trickl, Thomas and Vogel, Alfred},
   title = {Wavelength dependence of nanosecond infrared laser-induced breakdown in water: Evidence for multiphoton initiation via an intermediate state},
   journal = {Physical Review B},
   volume = {91},
   number = {13},
   pages = {134114},
   note = {PRB},
   DOI = {doi/10.1103/PhysRevB.91.134114},
   url = {http://link.aps.org/doi/10.1103/PhysRevB.91.134114},
   year = {2015},
   type = {Journal Article}
}

@article{Niu2015,
   author = {Niu, C. J. and Fisher, C. and Scheffler, K. and Wan, R. and Maleki, H. and Liu, H. and Sun, Y. and A. Simmons, C. and Birngruber, R. and Lilge, L.},
   title = {Polyacrylamide gel substrates that simulate the mechanical stiffness of normal and malignant neuronal tissues increase protoporphyin IX synthesis in glioma cells},
   journal = {Journal of Biomedical Optics},
   volume = {20(9)},
   Year = { 2015},
   DOI = {10.1117/1.JBO.20.9.098002},
  
   type = {Journal Article}
}

@article{Steiner2015,
   author = {Steiner, Patrick and Ebneter, Andreas and Berger, Lieselotte Erika and Zinkernagel, Martin and Považay, Boris and Meier, Christoph and Kowal, Jens H. and Framme, Carsten and Brinkmann, Ralf and Wolf, Sebastian and Sznitman, Raphael},
   title = {Time-Resolved Ultra–High Resolution Optical Coherence Tomography for Real-Time Monitoring of Selective Retina TherapyTime-Resolved Ultra–High Resolution OCT During SRT},
   journal = {Investigative Ophthalmology & Visual Science},
   volume = {56},
   number = {11},
   pages = {6654-6662},
   note = {10.1167/iovs.15-17151},
   abstract = {Abstract Purpose: Selective retina therapy (SRT) is a novel treatment for retinal pathologies, solely targeting the RPE. During SRT, the detection of an immediate tissue reaction is challenging, as tissue effects remain limited to intracellular RPE photodisruption. Time-resolved ultra-high axial resolution optical coherence tomography (OCT) is thus evaluated for the monitoring of dynamic optical changes at and around the RPE during SRT. Methods: An experimental OCT system with an ultra-high axial resolution of 1.78 μm was combined with an SRT system and time-resolved OCT M-scans of the target area were recorded from four patients undergoing SRT. Optical coherence tomography scans were analyzed and OCT morphology was correlated with findings in fluorescein angiography, fundus photography, and cross-sectional OCT. Results: In cases in which the irradiation caused RPE damage proven by fluorescein angiography, the lesions were well discernible in time-resolved OCT images but remained invisible in fundus photography and cross-sectional OCT acquired after treatment. If RPE damage was introduced, all applied SRT pulses led to detectable signal changes in the time-resolved OCT images. The extent of optical signal variation seen in the OCT data appeared to scale with the applied SRT pulse energy. Conclusions: The first clinical results proved that successful SRT irradiation induces detectable changes in the OCT M-scan signal while it remains invisible in conventional ophthalmoscopic imaging. Thus, real-time high-resolution OCT is a promising modality to monitor and analyze tissue effects introduced by selective retina therapy and may be used to guide SRT in an automatic feedback mode (www.swissmedic.ch number, 2011-MD-0006).},
   ISSN = {1552-5783},
   DOI = {10.1167/iovs.15-17151},
   year = {2015},
   type = {Journal Article}
}

@article{Koinzer2015,
   author = {Koinzer, S. and Baade, A. and Schlott, K. and Hesse, C. and Caliebe, A. and Roider, J. and Brinkmann, R.},
   title = {Temperature-Controlled Retinal Photocoagulation Reliably Generates Uniform Subvisible, Mild, or Moderate Lesions},
   journal = {Transl Vis Sci Technol},
   volume = {4},
   number = {5},
   pages = {9},
   note = {Koinzer, Stefan
Baade, Alexander
Schlott, Kerstin
Hesse, Carola
Caliebe, Amke
Roider, Johann
Brinkmann, Ralf
Journal article
Transl Vis Sci Technol. 2015 Oct 6;4(5):9. eCollection 2015 Oct.},
   abstract = {PURPOSE: Conventional retinal photocoagulation produces irregular lesions and does not allow reliable control of ophthalmoscopically invisible lesions. We applied automatically controlled retinal photocoagulation, which allows to apply uniform lesions without titration, and aimed at five different predictable lesion intensities in a study on rabbit eyes. METHODS: A conventional 532-nm photocoagulation laser was used in combination with a pulsed probe laser. They facilitated real-time fundus temperature measurements and automatic exposure time control for different predefined time/temperature dependent characteristics (TTC). We applied 225 control lesions (exposure time 200 ms) and 794 TTC lesions (5 intensities, exposure times 7-800 ms) in six rabbit eyes with variable laser power (20-66.4 mW). Starting after 2 hours, we examined fundus color and optical coherence tomographic (OCT) images over 3 months and classified lesion morphologies according to a seven-stage OCT classifier. RESULTS: Visibility rates in funduscopy (OCT) after 2 hours were 17% (68%) for TTC intensity group 1, 38% (90%) for TTC group 2 and greater than 94% (>98%) for all consecutive groups. TTC groups 1 through 4 correlated to increasing morphological lesion intensities and increasing median funduscopic and OCT diameters. Group 5 lesions were as large as, but more intense than group 4 lesions. CONCLUSIONS: Automatic, temperature controlled photocoagulation allows to apply predictable subvisible, mild, or moderate lesions without manual power titration. TRANSLATIONAL RELEVANCE: The technique will facilitate standardized, automatically controlled low and early treatment of diabetic retinopathy study (ETDRS) intensity photocoagulation independently of the treating physician, the treated eye and lesion location.},
   keywords = {Oct
animal model
laser photocoagulation
optoacoustics
real-time temperature measurement
spectral domain
sub-visible},
   ISSN = {2164-2591 (Print)
2164-2591},
   DOI = {10.1167/tvst.4.5.9},
   year = {2015},
   type = {Journal Article}
}

@article{Lange2015,
   author = {Lange, B. and Cordes, J. and Brinkmann, R.},
   title = {Stone/tissue differentiation for holmium laser lithotripsy using autofluorescence},
   journal = {Lasers Surg Med},
   volume = {47},
   number = {9},
   pages = {737-44},
   note = {1096-9101
Lange, Birgit
Cordes, Jens
Brinkmann, Ralf
Journal Article
United States
Lasers Surg Med. 2015 Nov;47(9):737-44. doi: 10.1002/lsm.22418. Epub 2015 Sep 22.},
   abstract = {BACKGROUND AND OBJECTIVES: Holmium laser lithotripsy is a safe and effective method to disintegrate urinary stones of all compositions in an endoscopic procedure. However, handling and safety could be improved by a real-time feedback system permanently monitoring the position of the treatment fiber. The laser is fired only when the fiber is identified as being placed in front of stone. This work evaluates the potential of fluorescence detection with an excitation wavelength of 532 nm for this purpose. MATERIALS AND METHODS: A fiber-based fluorescence measurement was set-up to acquire autofluorescence signals from several human renal calculi, artificial stones, and porcine tissue samples (renal calix and ureter). Three different approaches were investigated. First, experiments were performed with a pulsed laser source with a wavelength of 532 nm, pulse energy 36.5 +/- 1 muJ, pulse duration 1.2 +/- 0.5 nanoseconds, and a repetition rate of 1 kHz with 15 urinary concretions. In the second step, a series of measurements on 42 human urinary calculi samples was carried out using low power continuous wave excitation of 0.4 +/- 0.1 mW. Fluorescence was also measured simultaneously to stone fragmentation by holmium laser pulses (pulse energy 240 +/- 50 mJ, repetition rate 10 Hz). Finally, a modulated excitation/detection scheme (lock-in technique) was implemented to render fluorescence detection insensitive to white background light. RESULTS: Unlike porcine renal calix, ureter, and artificial stone human urinary calculi show a strong fluorescence signal when excited with 532 nm. With pulsed excitation on urinary stone (20,000 +/- 11,000) counts were registered at 587 nm with the CCD-array of a grating spectrometer in an integration time of 50 milliseconds. Tissue gave lower count rates of </=(5,500 +/- 1,100) even with longer integration times (500 milliseconds/1 second). With a cw excitation power of 0.4 mW (13,000 +/- 11,000) counts were registered in an integration time of 200 milliseconds at 587 nm (porcine renal calix: (770 +/- 340)). Modulated excitation (66 Hz) with an average power of 0.3 mW and detection with a photodiode resulted in a lock-in amplifier signal of 1.5-4.3V on stone (background and skin: <0.5V). CONCLUSION: With the lock-in technique, autofluorescence from stones can be detected with only the average excitation power of a green aiming beam overlaid to the Ho:YAG-laser beam (power </= 1 mW). Since tissue shows very little autofluorescence when excited with 532 nm, this fluorescence signal enables monitoring of the correct position of the treatment fiber during ureteroscopic procedures. Lasers Surg. Med. 47:737-744, 2015. (c) 2015 Wiley Periodicals, Inc.},
   keywords = {Holmium laser
feedback control
fluorescence
laser lithotripsy},
   ISSN = {0196-8092},
   DOI = {10.1002/lsm.22418},
   year = {2015},
   type = {Journal Article}
}

@article{Buj2015,
   author = {Buj, C and Horstmann, J and Münter, M and Brinkman, R},
   title = {Speckle-based off-axis holographic detection for non-contact photoacoustic tomography},
   journal = {Current Directions in Biomedical Engineering},
   volume = {1},
   pages = {356-360},
   year = {2015},
   type = {Journal Article}
}

@article{Wang2015,
   author = {Wang, S. and Huttmann, G. and Zhang, Z. and Vogel, A. and Birngruber, R. and Tangutoori, S. and Hasan, T. and Rahmanzadeh, R.},
   title = {Light-Controlled Delivery of Monoclonal Antibodies for Targeted Photoinactivation of Ki-67},
   journal = {Mol Pharm},
   note = {1543-8392
Wang, Sijia
Huttmann, Gereon
Zhang, Zhenxi
Vogel, Alfred
Birngruber, Reginald
Tangutoori, Shifalika
Hasan, Tayyaba
Rahmanzadeh, Ramtin
Journal article
Mol Pharm. 2015 Aug 13.},
   abstract = {The selective inhibition of intracellular and nuclear molecules such as Ki-67 holds great promise for the treatment of cancer and other diseases. However, the choice of the target protein and the intracellular delivery of the functional agent remain crucial challenges. Main hurdles are (a) an effective delivery into cells, (b) endosomal escape of the delivered agents, and (c) an effective, externally triggered destruction of cells. Here we show a light-controlled two-step approach for selective cellular delivery and cell elimination of proliferating cells. Three different cell-penetrating nano constructs, including liposomes, conjugates with the nuclear localization sequence (NLS), and conjugates with the cell penetrating peptide Pep-1, delivered the light activatable antibody conjugate TuBB-9-FITC, which targets the proliferation associated protein Ki-67. HeLa cells were treated with the photosensitizer benzoporphyrin monoacid derivative (BPD) and the antibody constructs. In the first optically controlled step, activation of BPD at 690 nm triggered a controlled endosomal escape of the TuBB-9-FITC constructs. In more than 75% of Ki-67 positive, irradiated cells TuBB-9-FITC antibodies relocated within 24 h from cytoplasmic organelles to the cell nucleus and bound to Ki-67. After a second light irradiation at 490 nm, which activated FITC, cell viability decreased to approximately 13%. Our study shows an effective targeting strategy, which uses light-controlled endosomal escape and the light inactivation of Ki-67 for cell elimination. The fact that liposomal or peptide-assisted delivery give similar results leads to the additional conclusion that an effective mechanism for endosomal escape leaves greater variability for the choice of the delivery agent.},
   keywords = {endosomal entrapment
liposome
nanotechnology
nuclear localization sequence (NLS)
photodynamic therapy},
   ISSN = {1543-8384},
   DOI = {10.1021/acs.molpharmaceut.5b00260},
   year = {2015},
   type = {Journal Article}
}

@article{Hüttmann2015,
   author = {Huttmann, Gereon and Koinzer, Stefan Otto Johannes and Müller, Heike and Ellerkamp, Iris and Baade, Alex and Moltmann, Moritz and Theisen-Kunde, Dirk and Lange, Birgit and Brinkmann, Ralf and Birngruber, Reginald},
   title = {Predicting ophthalmoscopic visibility of retinal photocoagulation lesions byhigh-speedOCT: an animal studyinrabbits},
   journal = {Investigative Ophthalmology & Visual Science},
   volume = {56},
   number = {7},
   pages = {5980-5980},
   ISSN = {1552-5783},
   year = {2015},
   type = {Journal Article}
}

@article{Kim2015,
   author = {Kim, H. D. and Han, J. W. and Ohn, Y. H. and Brinkmann, R. and Park, T. K.},
   title = {Functional evaluation using multifocal electroretinogram after selective retina therapy with a microsecond-pulsed laser},
   journal = {Invest Ophthalmol Vis Sci},
   volume = {56},
   number = {1},
   pages = {122-31},
   note = {1552-5783
Kim, Hoon Dong
Han, Jung Woo
Ohn, Young-Hoon
Brinkmann, Ralf
Park, Tae Kwann
Journal Article
United States
Invest Ophthalmol Vis Sci. 2014 Dec 11;56(1):122-31. doi: 10.1167/iovs.14-15132.},
   abstract = {PURPOSE: To evaluate the changes of retinal function with multifocal electroretinogram (mfERG), and estimate the association between functional and structural changes after selective retina therapy (SRT) with microsecond-pulsed laser in comparison to continuous wave laser photocoagulation (cwPC). METHODS: Selective retina therapy and cwPC were applied with 10 x 10 shots and 1/2 lesion-width on the retina in the right and left eyes of 20 healthy Chinchilla Bastard rabbits, respectively. Optical coherence tomography (OCT), fundus fluorescein angiography (FFA), and mfERG were performed before, and on days 1, 7, and 30 after both laser treatments. The mean ratios of amplitudes and implicit times of N1 and P1 from eight hexagons covering laser-treated retinal lesions/total retina were measured. Histology was obtained after killing three rabbits at each time period to observe the anatomic changes after both laser treatments. RESULTS: The mean ratios of amplitudes of N1 and P1 in SRT lesions did not change significantly for 30 days after laser treatment. Only subtle reductions of the mean ratios of N1 and P1 amplitudes on day 1, thereafter the amplitudes showed the trend to recover toward baseline values. Histology and OCT revealed temporary and reversible morphologic changes after SRT, which restored to normal within 1 month. However, the mean ratios of N1 amplitudes on days 7 and 30 (P = 0.010, P < 0.001, respectively), and P1 amplitudes on days 7 and 30 (P < 0.001, P < 0.001, respectively) declined significantly in cwPC lesions compared with baseline. Disorganization and atrophic changes were identified on histology and OCT after cwPC. CONCLUSIONS: The results suggest that SRT preserved retinal function as well as anatomical structure after treatment.},
   keywords = {continuous wave laser photocoagulation (cwPC)
multifocal electroretinogram (mfERG)
selective retina therapy (SRT)},
   ISSN = {0146-0404},
   DOI = {10.1167/iovs.14-15132},
   year = {2015},
   type = {Journal Article}
}

@article{Park2015,
   author = {Park, Young Gun and Kang, Seungbum and Brinkmann, Ralf and Roh, Young-Jung},
   title = {A Comparative Study of Retinal Function in Rabbits after Panretinal Selective Retina Therapy versus Conventional Panretinal Photocoagulation},
   journal = {Journal of Ophthalmology},
   volume = {2015},
   pages = {8},
   DOI = {10.1155/2015/247259},
   url = {http://dx.doi.org/10.1155/2015/247259},
   year = {2015},
   type = {Journal Article}
}

@article{Apiou-Sbirlea2015,
   author = {Apiou-Sbirlea, Gabriela and Tearney, Guillermo J. and Birngruber, Reginald and Hasan, Tayyaba and Anderson, Richard Rox},
   title = {Anatomy and physiology of translation: the academic research imperative},
   journal = {Clinical Investigation},
   volume = {5},
   number = {10},
   pages = {797-804},
   ISSN = {2041-6792},
   DOI = {10.4155/cli.15.46},
   year = {2015},
   type = {Journal Article}
}

title = {Non-Invasive Multi-Dimensional Two-Photon Microscopy enables optical fingerprinting (TPOF) of immune cells},
journal = {Journal of Biophotonics},
volume = {8},
number = {6},
pages = {466-479},
keywords = {ocular surface, intravital two-photon microscopy, antigen presenting cells, in vivo, non invasive},
doi = {https://doi.org/10.1002/jbio.201400036},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201400036},
eprint = {https://onlinelibrary.wiley.com/doi/pdf/10.1002/jbio.201400036},
abstract = {Mucosal surfaces are constantly exposed to pathogens and show high immunological activity. In a broad variety of ocular surface disorders inflammation is common, but underlying mechanisms are often not fully understood. However, the main clinical problem is that inflammatory processes are difficult to characterize and quantify due to the impossibility of repeated tissue probing of the delicate ocular surface. Therefore non-invasive optical methods are thought to have the potential for intravital investigation of ocular surface inflammation. This study demonstrates the general potential of two-photon microscopy to non-invasively detect and discriminate key players of inflammation in the ocular surface by using intrinsic fluorescence-based features without the necessity of tissue probing or the use of dyes. The use of wavelength dependent measurements of fluorescence lifetime, in addition to autofluorescence intensity enables a functional differentiation of isolated immune cells in vitro at excitation wavelengths between 710 to 830 nm. Mixed cell cultures and first in vivo results indicate the use of excitation wavelength of 710 to 750 nm for further experiments and future use in patients. Two photon based autofluorescence features of immune cells enables non-invasive differentiation.},
year = {2015}
}

@article{Horstmann2015,
   title        = {Full-field speckle interferometry for non-contact photoacoustic tomography},
   author       = {Horstmann, J. and Spahr, H. and Buj, C. and Munter, M. and Brinkmann, R.},
   journal      = {Phys Med Biol},
   volume       = 60,
   number       = 10,
   pages        = {4045--58},
   note         = {1361-6560 abstract = {A full-field speckle interferometry method for non-contact and prospectively high speed Photoacoustic Tomography is introduced and evaluated as proof of concept. Thermoelastic pressure induced changes of the objects topography are acquired in a repetitive mode without any physical contact to the object. In order to obtain high acquisition speed, the object surface is illuminated by laser pulses and imaged onto a high speed camera chip. In a repetitive triple pulse mode, surface displacements can be acquired with nanometre sensitivity and an adjustable sampling rate of e.g. 20 MHz with a total acquisition time far below one second using kHz repetition rate lasers. Due to recurring interferometric referencing, the method is insensitive to thermal drift of the object due to previous pulses or other motion. The size of the investigated area and the spatial and temporal resolution of the detection are scalable. In this study, the approach is validated by measuring a silicone phantom and a porcine skin phantom with embedded silicone absorbers. The reconstruction of the absorbers is presented in 2D and 3D. The sensitivity of the measurement with respect to the photoacoustic detection is discussed. Potentially, Photoacoustic Imaging can be brought a step closer towards non-anaesthetized in vivo imaging and new medical applications not allowing acoustic contact, such as neurosurgical monitoring or burnt skin investigation.}, ISSN = {0031-9155}, DOI = {10.1088/0031-9155/60/10/4045}, year = {2015}, type = {Journal Article}}
}

@article{Han2015,
   author = {Han, Bing and Köhler, Karsten and Jungnickel, Kerstin and Mettin, Robert and Lauterborn, Werner and Vogel, Alfred},
   title = {Dynamics of laser-induced bubble pairs},
   journal = {Journal of Fluid Mechanics},
   volume = {771},
   pages = {706-742},
   keywords = {bubble dynamics,cavitation,jets},
   ISSN = {1469-7645},
   DOI = {doi:10.1017/jfm.2015.183},
   url = {http://dx.doi.org/10.1017/jfm.2015.183},
   year = {2015},
   type = {Journal Article}
}